In vitro effects of estrogens on rat prostatic 5α-reduction of testosterone

Lee, D.K.H.; Bird, C. E.; Clark, A. F.

doi:10.1016/0039-128x(73)90115-3

Cited by 18 publications

(6 citation statements)

References 17 publications

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“…This is in agreement with a previous report on the influence of Oe2 treatment on 5a-reductase activity (Fencle & Villee 1973;Yamanaka et al 1975). Oe2 is a known strong inhibitor of 5ct-reduction of T in vitro (Lee et al 1973;Kadohama et al 1977), butin the castrated animal this type of inhibition may not be of importance. In contrast to the findings of Leav et al (1971) that Oe2 administration to dogs leads to a shift from reductive to oxidative meta¬ bolism of T, we observed some inhibition of I7ß-hydroxy oxidation of T in Oe2-treated rats.…”

Section: )mentioning

confidence: 95%

A model for studies on the response of the ventral prostate to oestrogens

Corrales

Høisæter

Kadohama

et al. 1981

Acta Endocrinologica

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The effects of oestrogen administration on the weight of ventral and dorsolateral prostates were studied in castrated rats of Wistar-Furth and Copenhagen strains. Direct effect of oestradiol (Oe2) on prostatic tissue was also investigated in organ culture. Oe2\x=req-\ treated animals received daily injections of 50 \g=m\g for 7 days. Control animals were treated with the vehicle only (peanut oil). In 4 month old Copenhagen rats the mean weight of the ventral prostates (42.4 \ m=+-\ 9.4 mg/100 g body weight) was significantly higher than that in the control animals (19.9 \m=+-\4.6 mg/100 g body weight, P < 0.0001). No such differences were observed in older Copenhagen rats (9 months old) or Wistar-Furth rats (3 and 6 months old). Thus, this effect of Oe2 on ventral prostate seems both strain specific and age specific. Similar strain and age differences in the Oe2 effect were found in the dorsolateral prostate, but to a smaller extent. Direct interaction of Oe2 with target tissue was demonstrated in culture as evidenced by the ability of the steroid to prevent regression in explants derived from prostates of Copenhagen rats. The in vivo effects of Oe2 on the prostate weights could not be explained by differences in specific androgen or oestrogen receptor contents or in testosterone (T) metabolism. However, the prostates of younger Copenhagen rats differed from those of all other groups in three respects: 1) they contained high levels of oestramustine binding protein (OeBP), 2) they had the highest amount of uptake of radioactivity into the nuclear residue, and 3) their histological picture was characterized by diffuse stromal architecture having the appearance of oedematous tissue.

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Section: )mentioning

confidence: 95%

A model for studies on the response of the ventral prostate to oestrogens

Corrales

Høisæter

Kadohama

et al. 1981

Acta Endocrinologica

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“…Estradiol (E2) is reported to be the most potent naturally occurring estrogen [5]. It enters cells freely, binds to cytoplasmic receptors, and the complexes then move to the nucleus to bind to chromatin acceptor sites, producing the effects on the target cells [6].…”

Section: Introductionmentioning

confidence: 99%

Simvastatin Maintains Osteoblastic Viability While Promoting Differentiation by Partially Regulating the Expressions of Estrogen Receptors α

Park

Zhang

Lin

et al. 2012

Journal of Surgical Research

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“…on this enzyme is well known and has been documented several times (Lee et al 1973a,b;Shimazaki et al 1971Shimazaki et al , 1972. Lee et al (1973 ) found that only those oestrogens with a complete steroid structure and a free phenolic hydroxyl group at position 3 inhibited the 5a-reductase activity in ventral prostate homogenate. 1971;Altwein et al 1974).…”

Section: Discussionmentioning

confidence: 90%

The EFFECT OF OESTRADIOL-3N-BIS-(2-CHLOROETHYL)CARBAMATE-17β-PHOSPHATE (ESTRACYT®) ON THE 5α-Reductase IN THE RAT VENTRAL PROSTATE

Høisæter¹

1975

Acta Endocrinologica

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The ventral prostate of the rat both after in vitro incubation and in vivo experiments was found to contain appreciable 5\g=a\-reductaseactivity, whilst a very low activity was registered in the diaphragm and liver. Neither Estracyt\s=r\ nor LEO275 (Estracyt\s=r\ without the phosphate group in position 17 of the oestradiol moiety) had an inhibitory effect on the enzyme activity after in vitro incubation but equivalent amounts of oestradiol-17\g=b\ and oestradiol-17\g=b\-phosphatesignificantly reduced 5\ g=a\ \ x=r eq-\ reductase activity. When Estracyt\s=r\ was injected in vivo no influence on activity was registered in "short term" experiments while a significant inhibition was found after "long term" treatment in vivo. Possible explanations for this "long term" effect of Estracyt\s=r\ on 5\g=a\-reductase activity are discussed.

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In vitro effects of estrogens on rat prostatic 5α-reduction of testosterone

Cited by 18 publications

References 17 publications

A model for studies on the response of the ventral prostate to oestrogens

A model for studies on the response of the ventral prostate to oestrogens

Simvastatin Maintains Osteoblastic Viability While Promoting Differentiation by Partially Regulating the Expressions of Estrogen Receptors α

The EFFECT OF OESTRADIOL-3N-BIS-(2-CHLOROETHYL)CARBAMATE-17β-PHOSPHATE (ESTRACYT®) ON THE 5α-Reductase IN THE RAT VENTRAL PROSTATE

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