In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation

Anjamrooz, Seyed Hadi; Movahedin, Mansoureh; Tiraihi, Taki; Mowla, Seyed Javad

doi:10.1071/rd05126

Cited by 26 publications

(15 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is in agreement with that reported by previous studies that cultured mouse SSCs Jeong et al 2003) and SSCs in the colonies (Anjamrooz et al 2006) can result in a spermatogenesis following transplantation.…”

Section: Discussionsupporting

confidence: 95%

“…These systems include SSCs co-culture with a Sertoli cells (Anjamrooz et al 2006;Aponte et al 2006) or SIM mouse embryo-derived thioguanine-and ouabain-resistant (STO) monolayer (Kubota et al 2004a) as feeder cells, addition of serum into the culture medium , using serum-free culture medium, SSCs cultured in a potassium-rich medium called KSOM (Dirami et al 1999) and medium supplementation with various growth factors (Kanatsu-Shinohara et al 2003a, b;Kubota et al 2004b). Recently, SSCs were also isolated and cultured from adult mice (Guan et al 2006) but optimal conditions for in vitro colonization of adult SSCs is still unclear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficiency of adult mouse spermatogonial stem cell colony formation under several culture conditions

Koruji

Movahedin

Mowla

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

The aim of this study was to compare the in vitro effects of glial cell line-derived neurotrophic factor, stem cell factor, granulocyte macrophage-colony stimulating factor, and co-culture with Sertoli cells on the efficiency of adult mouse spermatogonial stem cells colony formation. For these purpose, both Sertoli and spermatogonial cells were isolated from adult mouse testes. The identity of the cells was confirmed through analysis of alkaline phosphatase activity, immunocytochemistry against OCT-4, c-kit, and vimentin, and also by transplantation of these cells in the recipient testes. The isolated spermatogonial cells were treated either with various concentrations of the above mentioned factors or co-cultured with Sertoli cells for 3 wk. The spermatogonial cells of the resulting colonies were transplanted via rete testis into the mouse testes, which were irradiated with 14 Gy. The results indicated that glial cell line-derived neurotrophic factor is the most appropriate factor for in vitro colonization of adult mice spermatogonial cells compared with other cytokines and growth factors. A short-term co-culture with Sertoli cells showed a significant increase in the number and diameter of the colonies compared with the treated growth factors and the control group. We have also demonstrated that mouse spermatogonial stem cells in the colonies after co-culturing with Sertoli cells could induce spermatogenesis in the recipient testes after transplantation.

show abstract

Section: Discussionsupporting

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Efficiency of adult mouse spermatogonial stem cell colony formation under several culture conditions

Koruji

Movahedin

Mowla

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

show abstract

“…For instance, under the influence of EGF and FGF2, big odd-shaped colonies appeared. Stereological analysis of the colonies indicated that although EGF has been described as a spermatogonial growth factor (WahabWahlgren et al 2003, Anjamrooz et al 2006, the cultures with this growth factor showed the largest volume of somatic cells. In EGF-treated cultures, spherical colonies arose interconnected by bridges.…”

Section: Discussionmentioning

confidence: 99%

Propagation of bovine spermatogonial stem cells in vitro

Aponte¹,

Soda²,

Teerds³

et al. 2008

Reproduction

138

113

View full text Add to dashboard Cite

The access to sufficient numbers of spermatogonial stem cells (SSCs) is a prerequisite for the study of their regulation and further biomanipulation. A specialized medium and several growth factors were tested to study the in vitro behavior of bovine type A spermatogonia, a cell population that includes the SSCs and can be specifically stained for the lectin Dolichos biflorus agglutinin. During short-term culture (2 weeks), colonies appeared, the morphology of which varied with the specific growth factor(s) added. Whenever the stem cell medium was used, round structures reminiscent of sectioned seminiferous tubules appeared in the core of the colonies. Remarkably, these round structures always contained type A spermatogonia. When leukemia inhibitory factor (LIF), epidermal growth factor (EGF), or fibroblast growth factor 2 (FGF2) were added, specific effects on the numbers and arrangement of somatic cells were observed. However, the number of type A spermatogonia was significantly higher in cultures to which glial cell line-derived neurotrophic factor (GDNF) was added and highest when GDNF, LIF, EGF, and FGF2 were all present. The latter suggests that a proper stimulation of the somatic cells is necessary for optimal stimulation of the germ cells in culture. Somatic cells present in the colonies included Sertoli cells, peritubular myoid cells, and a few Leydig cells. A transplantation experiment, using nude mice, showed the presence of SSCs among the cultured cells and in addition strongly suggested a more than 10 000-fold increase in the number of SSCs after 30 days of culture. These results demonstrate that bovine SSC self-renew in our specialized bovine culture system and that this system can be used for the propagation of these cells.

show abstract

“…During spermatogenesis, Sertoli cells and reproductive hormones, such as testosterone, follicle‐stimulating hormone (FSH), and luteinizing hormone (LH), play important roles. Meanwhile, it has been reported that several cytokines, including epidermal growth factor (EGF), stem cell factor (SCF), glial derived neurotrophic factor (GDNF), and insulin‐like growth factor (IGF), were also crucial for spermatogenesis .…”

Section: Introductionmentioning

confidence: 99%

Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition

Deng

Chen

Wang

et al. 2016

Journal of Pineal Research

View full text Add to dashboard Cite

Promotion of spermatogonial stem cell (SSC) differentiation into functional sperms under in vitro conditions is a great challenge for reproductive physiologists. In this study, we observed that melatonin (10−7 m) supplementation significantly enhanced the cultured SSCs differentiation into haploid germ cells. This was confirmed by the expression of sperm special protein, acrosin. The rate of SSCs differentiation into sperm with melatonin supplementation was 11.85 ± 0.93% which was twofold higher than that in the control. The level of testosterone, the transcriptions of luteinizing hormone receptor (LHR), and the steroidogenic acute regulatory protein (StAR) were upregulated with melatonin treatment. At the early stage of SSCs culture, melatonin suppressed the level of cAMP, while at the later stage, it promoted cAMP production. The similar pattern was observed in testosterone content. Expressions for marker genes of meiosis anaphase, Dnmt3a, and Bcl‐2 were upregulated by melatonin. In contrast, Bax expression was downregulated. Importantly, the in vitro‐generated sperms were functional and they were capable to fertilize oocytes. These fertilized oocytes have successfully developed to the blastula stage.

show abstract

In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation

Cited by 26 publications

References 39 publications

Efficiency of adult mouse spermatogonial stem cell colony formation under several culture conditions

Efficiency of adult mouse spermatogonial stem cell colony formation under several culture conditions

Propagation of bovine spermatogonial stem cells in vitro

Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition

Contact Info

Product

Resources

About