In vitro effects of different steroid hormones on superoxide anion production of human neutrophil granulocytes

Bekesi, Gabor; Kakucs, Réka; Várbı́ró, Szabolcs; Rácz, Kàroly; Sprintz, Detlef; Fehér, János; Székács, Béla

doi:10.1016/s0039-128x(00)00183-5

Cited by 100 publications

(36 citation statements)

References 30 publications

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“…The lucigenin chemiluminescence measurement showed that ACh-induced formation of superoxide anion was greater in segments from orchidectomized than control rats. This result agrees with a study carried out in mesenteric artery and with previous studies showing the antioxidant properties of androgens (Békési et al 2000, Yorek et al 2002.…”

Section: Discussionsupporting

confidence: 93%

“…However, tempol induced slight or strong increase in the vasodilator response to SNP respectively in arteries from control or orchidectomized rats. These results indicate a higher NO metabolism in arteries from orchidectomized rats, as we have found in mesenteric artery and reinforced the described antioxidant properties of androgens (Békési et al 2000, Yorek et al 2002. Additionally, the lack of effect of exogenous SOD would indicate that the predominant isoform involved in this effect would be the cytosolic Cu/Zn-SOD.…”

Section: Discussionsupporting

confidence: 86%

“…In this context, it has been reported that the expression and/or activity of SODs are increased in pathological conditions like atherosclerosis (Kobayashi et al 2002), in which the production of superoxide anions is increased (Wolin 2002). On the other hand, several studies have demonstrated androgen antioxidant properties (Békési et al 2000, Yorek et al 2002 and we observed an increased superoxide anion production in mesenteric artery from orchidectomized rats that had an increased neuronal NO metabolism . In view of these data, we hypothesized that endogenous male sex hormones could influence the production of superoxide anion, as well as the expression and/or activity of SOD.…”

Section: Introductionsupporting

confidence: 49%

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Orchidectomy increases expression and activity of Cu/Zn-superoxide dismutase, while decreasing endothelial nitric oxide bioavailability

Blanco-Rivero¹,

Sagredo²,

Balfagón³

et al. 2006

Journal of Endocrinology

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This study examines the effect of male sex hormones on the release, metabolism and function of endothelial nitric oxide (eNO) in rat aorta. Aortic segments from orchidectomized and control male Sprague-Dawley rats were used to measure eNO synthase (eNOS) expression, nitric oxide (NO) release, acetylcholine (ACh)-and sodium nitroprusside (SNP)-induced relaxation and Cu/Zn-superoxide dismutase (SOD) expression and activity. eNOS expression as well as basal and ACh-induced NO release were similar in arteries from both groups of rats. Basal superoxide anion production was similar in arteries from both groups, while ACh-induced superoxide anion formation was greater in arteries from orchidectomized than control rats. Orchidectomy increased the vasodilator effect induced by ACh, but did not alter that induced by SNP. SOD, a superoxide anion scavenger, did not modify the SNP-induced relaxation in aortas from control or orchidectomized rats. The membrane-permeable mimetic of SOD, tempol, increased the SNP-induced relaxation more in aortas from orchidectomized than control rats. The effect of endogenous SOD inhibitor, diethyl-dithiocarbamate, reduced the relaxation induced by SNP in segments from both groups of rats. The expression and activity of Cu/Zn-SOD were greater in aortas from orchidectomized than control rats. These data show that endogenous male sex hormone deprivation altered neither eNOS expression nor eNO release, while it increased the expression and activity of Cu/Zn-SOD. However, the predominant vascular effect of orchidectomy is to increase NO metabolism.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Introductionsupporting

confidence: 49%

See 1 more Smart Citation

Orchidectomy increases expression and activity of Cu/Zn-superoxide dismutase, while decreasing endothelial nitric oxide bioavailability

Blanco-Rivero¹,

Sagredo²,

Balfagón³

et al. 2006

Journal of Endocrinology

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show abstract

“…In addition, we observed that superoxide anion negatively modulates the vasodilator response to ACh only in arteries from orchidectomized rats, since SOD, a superoxide anion scavenger, or tempol, a membrane-permeable mimetic of endogenous SOD, increased the relaxation to ACh only in arteries from these rats. These results indicate a greater NO metabolism in arteries from orchidectomized rats and are in line with reports describing the antioxidant properties of androgens (Békési et al 2000, Yorek et al 2002, Martín et al 2005. Additionally, these results also indicate that the localization of the SOD involved in this effect would be predominantly extracellular.…”

Section: Discussionsupporting

confidence: 92%

Protein kinase C activation increases endothelial nitric oxide release in mesenteric arteries from orchidectomized rats

Blanco-Rivero¹,

Sagredo²,

Balfagón³

et al. 2007

Journal of Endocrinology

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The aim of the present study was to assess the effect of endogenous male sex hormones on endothelial nitric oxide synthase (eNOS) expression, release and function of the endothelial nitric oxide (NO), as well as to assess the regulatory action of protein kinase C (PKC) on acetylcholine (ACh)-induced endothelial NO release. For this purpose, superior mesenteric arteries from control and orchidectomized male Sprague-Dawley rats were used. eNOS expression and basaland ACh-induced NO release were similar in arteries from both groups of rats. Orchidectomy decreased the vasodilator effect induced by ACh but did not alter that induced by sodium nitroprusside (SNP). The superoxide anion scavenger, superoxide dismutase (SOD), or the membrane-permeable mimetic of SOD, tempol, only enhanced ACh-induced relaxation in arteries from orchidectomized rats. ACh-induced TXA 2 formation was higher in arteries from orchidectomized than from control rats. Neither the PKC activator, phorbol 12,13-dibutyrate (PDBu), nor the non-selective PKC inhibitor, calphostin C, modified basal-or ACh-induced NO release in arteries from control rats. In arteries from orchidectomized rats, basal-and ACh-induced endothelial NO release were increased by PDBu but decreased by calphostin C. Both Gö 6976, a PKC inhibitor that is partially selective for conventional PKC isoforms, as well as PKCz pseudosubstrate inhibitor (PKCz-PI) decreased both basal-and ACh-induced NO release in arteries from orchidectomized rats. Neither PDBu nor calphostin C modified the vasodilator response induced by ACh in arteries from control rats. In segments from orchidectomized rats, PDBu enhanced the ACh-induced response, but this response was not modified by calphostin C, Gö6976 or PKCz-PI. The vasodilator response induced by SNP was not altered by the PKC activators or inhibitors in any artery from either group. These results show that endogenous male sex hormone deprivation does not affect the eNOS expression or the endothelial NO release induced by ACh, but does decrease the vasodilator action of ACh, by increasing NO metabolism and TXA 2 formation. In addition, PKC seems to modulate eNOS activity only in mesenteric arteries from orchidectomized rats, in which conventional and PKCz isoforms are involved in the positive regulation of eNOS.

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“…Oxidative stress could be a mechanism involved in progression of cardiovascular disease, in part for the oxidation of LDL-cholesterol [16], and in part for the activation of neutrophils, that contribute to ROS production through NADPH oxidase activation and release of myeloperoxidase, with a vicious circle inducing more toxic oxidant species. This mechanism can be counteracted by testosterone [17].…”

Section: Discussionmentioning

confidence: 99%

Effects of Intramuscular or Bioadhesive Buccal Testosterone Treatment on Antioxidant Systems in Secondary Hypogonadism

Raimondo¹

2013

J Steroids Horm Sci

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In vitro effects of different steroid hormones on superoxide anion production of human neutrophil granulocytes

Cited by 100 publications

References 30 publications

Orchidectomy increases expression and activity of Cu/Zn-superoxide dismutase, while decreasing endothelial nitric oxide bioavailability

Orchidectomy increases expression and activity of Cu/Zn-superoxide dismutase, while decreasing endothelial nitric oxide bioavailability

Protein kinase C activation increases endothelial nitric oxide release in mesenteric arteries from orchidectomized rats

Effects of Intramuscular or Bioadhesive Buccal Testosterone Treatment on Antioxidant Systems in Secondary Hypogonadism

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