In Vitro Effects of Cinc/Gro, a Member of the Interleukin-8 Family, on Interleukin-6 Secretion by Rat Posterior Pituitary Cells

Sawada, Takuya; Koike, Koji; Sakamoto, Yoshiki; Nohara, A.; Kanda, Yuki; Zhang, Zhen Xian; Ohmichi, Masahide; Yamaguchi, Masatoshi; Hirota, Kenji; Miyake, Akira

doi:10.1006/bbrc.1994.1513

Cited by 8 publications

(3 citation statements)

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“…It has been found to be induced to a higher extent in spinal cord than brain by IL-1␤, which is consistent with a more rapid accumulation of neutrophils after injury (4) or ischemia (40). Expression has been noted previously in vascular cells 4 h following spinal contusion (66), and it may induce IL-6 and other signaling molecules such as prolactin (55,56). We predict that NS398-induced expression of Gro may lead to enhanced neutrophil accumulation and localized damage following SCI but also may be involved in regulating immune response to injury.…”

Section: Discussionsupporting

confidence: 66%

Screening anti-inflammatory compounds in injured spinal cord with microarrays: a comparison of bioinformatics analysis approaches

Pan

Jörnsten

Hart

2004

Physiological Genomics

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Inflammatory responses contribute to secondary tissue damage following spinal cord injury (SCI). A potent anti-inflammatory glucocorticoid, methylprednisolone (MP), is the only currently accepted therapy for acute SCI but its efficacy has been questioned. To search for additional anti-inflammatory compounds, we combined microarray analysis with an explanted spinal cord slice culture injury model. We compared gene expression profiles after treatment with MP, acetaminophen, indomethacin, NS398, and combined cytokine inhibitors (IL-1ra and soluble TNFR). Multiple gene filtering methods and statistical clustering analyses were applied to the multi-dimensional data set and results were compared. Our analysis showed a consistent and unique gene expression profile associated with NS398, the selective cyclooxygenase-2 (COX-2) inhibitor, in which the overall effect of these upregulated genes could be interpreted as neuroprotective. In vivo testing demonstrated that NS398 reduced lesion volumes, unlike MP or acetaminophen, consistent with a predicted physiological effect in spinal cord. Combining explanted spinal cultures, microarrays, and flexible clustering algorithms allows us to accelerate selection of compounds for in vivo testing.

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Section: Discussionsupporting

confidence: 66%

Screening anti-inflammatory compounds in injured spinal cord with microarrays: a comparison of bioinformatics analysis approaches

Pan

Jörnsten

Hart

2004

Physiological Genomics

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“…The primary structure of CINC also has a 47.2% homology with human IL-8. Although CINC has been implicated in linking the immune and neuro-endocrine systems (47), its principal function is considered to be its neutrophil-specific chemotactic activity (48).…”

Section: Discussionmentioning

confidence: 99%

Gender differences in neutrophil function and cytokine-induced neutrophil chemoattractant generation in endotoxic rats

Spitzer

Zhang

1996

Inflammation

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Several lines of evidence indicate sexual dimorphism in the immune response. We explored gender differences in phagocytosis by neutrophils (PMNs), CD11b/c expression, generation of cytokine-induced neutrophil chemoattractant (CINC) and the influence of developmental stages on some of these parameters. Phagocytosis by PMNs of reproductive female rats was not suppressed by anesthesia and surgery as it was in age-matched males. The phagocytic response to an endotoxir (ET) challenge was also higher in PMNs of reproductive females than in males or in prereproductive or postreproductive females. CINC generation in reproductive females was lower than in age-matched males. Phagocytosis in saline-treated postreproductive females was reduced compared to reproductive and prereproductive females, but was not different from adult males. CD11b/c expression was greater in PMNs of saline treated postreproductive females, than in reproductive or prereproductive animals, but an ET challenge upregulated CD11b/c expression to the same level in all three groups. No gender difference was observed in this parameter. These data indicate that in terms of phagocytosis PMNs of reproductive female rats are more resistant to the effects of anesthesia and surgery and respond to an ET challenge more vigorously than cells of age-matched males. CINC generation in adult rats is also gender dependent. The developmental stages in females modulate phagocytosis and beta 2-integrin expression in PMNs.

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“…Challenges with ovalbumin (OVA) were found to induce an allergic reaction and increased CINC-1 levels in the paw skin of rats [22]. Normal posterior pituitary cells of unstimulated rats were shown to produce a small amount of CINC-1 (0.025-0.15 ng/ml; 3 × 10 −12 −2 × 10 −11 M) in a time-dependent manner [23]. The acute administration of cadmium to rats resulted in increased levels of CINC-1 in the rat liver [24].…”

Section: Agents or Conditions That Induce Cinc-1 Productionmentioning

confidence: 99%

Rat Cytokine-Induced Neutrophil Chemoattractant-1 (CINC-1) in Inflammation

Handa¹,

Naito²,

Yoshikawa³

2006

J. Clin. Biochem. Nutr.

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Summary Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1) belongs to the CXCchemokine family and is a counterpart of human growth-related oncogene (GRO) in the interleukin-8 (IL-8) family. In rats, CINC-1 plays a key role in neutrophil-mediated inflammatory diseases by attracting neutrophils to the site of inflammation. Although IL-8 is a well-known chemokine in humans, it remains difficult to investigate human inflammatory diseases in detail. Therefore CINC-1 is expected to be an excellent tool for investigating neutrophil-mediated inflammatory diseases, and it has served extensively as a target chemokine in studies performed to date. However, little is known regarding CINC-1 signaling pathways. In this review, we focused on the signal transduction of CINC-1 production/ expression.

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In Vitro Effects of Cinc/Gro, a Member of the Interleukin-8 Family, on Interleukin-6 Secretion by Rat Posterior Pituitary Cells

Cited by 8 publications

References 0 publications

Screening anti-inflammatory compounds in injured spinal cord with microarrays: a comparison of bioinformatics analysis approaches

Screening anti-inflammatory compounds in injured spinal cord with microarrays: a comparison of bioinformatics analysis approaches

Gender differences in neutrophil function and cytokine-induced neutrophil chemoattractant generation in endotoxic rats

Rat Cytokine-Induced Neutrophil Chemoattractant-1 (CINC-1) in Inflammation

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