In vitro effects of Cassava (Manihot esculenta) leaf extracts on four development stages of Haemonchus contortus

Marie-Magdeleine, Carine; Udino, Lyn; Philibert, Lucien; Bocage, Bruno; Archimède, Harry

doi:10.1016/j.vetpar.2010.06.017

Cited by 43 publications

(23 citation statements)

References 38 publications

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“…The in vitro egg hatch assay was based on the method of Marie-Magdeleine et al (2010). One mL of egg suspension was distributed in each well of 48 well plates containing ~400 eggs/well, and mixed with the same volume of plant extracts (i.e., 125, 250, 500 µg/mL).…”

Section: Eggs Hatch Assaymentioning

confidence: 99%

In VitroActivity ofPithecellobium DulceandLysiloma Acapulcensison Exogenous Development Stages of Sheep Gastrointestinal Strongyles

Olmedo-Juárez

Rubio

Arece-García

et al. 2014

Italian Journal of Animal Science

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An experiment was conducted to evaluate the effects of two lyophilised aqueous extracts of Lysiloma acapulcensis (LAE) and Phitecellobium dulce (PDE) tree leaves on in vitro assessment of hatching of eggs, larval development and migration of gastrointestinal nematodes of sheep using a general linear model. Treatments contained extracts from both species at concentrations of 0, 125, 250 and 500 µg/mL. Both albendazole and levamisole were used at a level of 1% as positive control. The extract of LAE, compared to PDE, showed better inhibition (P<0.05) of egg hatching. Different doses of both the LAE and PDE extracts showed a larvicidal effect (P<0.05) on all larvae exposed to different doses of the extracts. In the larval migration assay, a similar effect with levamisole at doses of 250 and 500 µg/mL occurred with the LAE extract. The extract of P. dulce had a lower larvicidal effect (P<0.05) than levamisole and L. acapulcensis extracts. Using aqueous extracts of both species of L. acapulcensis and P. dulce could be a promising alternative to synthetic anthelmintics as treatments of gastrointestinal nematodes of sheep in organic and conventional production systems under subtropical conditions.

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Section: Eggs Hatch Assaymentioning

confidence: 99%

In VitroActivity ofPithecellobium DulceandLysiloma Acapulcensison Exogenous Development Stages of Sheep Gastrointestinal Strongyles

Olmedo-Juárez

Rubio

Arece-García

et al. 2014

Italian Journal of Animal Science

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“…Gastrointestinal nematodes, especially Haemonchus contortus, cause economic losses in sheep and goat production worldwide (PERRY & RANDOLPH, 1999;MARIE-MAGDELEINE et al, 2010;QI et al, 2015). This nematode causes weight loss, decreases milk production, and reduces fertility, and may lead to death in cases of high infection (BESIER et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic effect of thymol and thymol acetate on sheep gastrointestinal nematodes and their toxicity in mice

André

Cavalcante

Ribeiro

et al. 2017

Rev. Bras. Parasitol. Vet.

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Thymol is a monoterpene and acetylation form of this compound can reduce the toxicity and enhance its biological effects. The objective of this study was to evaluate the effect of thymol and thymol acetate (TA) on egg, larva and adult Haemonchus contortus and the cuticular changes, acute toxicity in mice and the efficacy on sheep gastrointestinal nematodes. In vitro tests results were analyzed by analysis of variance (ANOVA) and followed by comparison with Tukey test or Bonferroni. The efficacy of in vivo test was calculated by the BootStreet program. In the egg hatch test (EHT), thymol (0.5 mg/mL) and TA (4 mg/mL) inhibited larval hatching by 98% and 67.1%, respectively. Thymol and TA (8 mg/mL) inhibited 100% of larval development. Thymol and TA (800 µg/mL) reduced the motility of adult worms, by 100% and 83.4%, respectively. Thymol caused cuticular changes in adult worm teguments. In the acute toxicity test, the LD 50 of thymol and TA were 1,350.9 mg/kg and 4,144.4 mg/kg, respectively. Thymol and TA reduced sheep egg count per gram of faeces (epg) by 59.8% and 76.2%, respectively. In in vitro tests thymol presented better anthelmintic activity than TA. However TA was less toxic and in in vivo test efficacy was similar.Keywords: Acetylation, thymol, Haemonchus contortus, nematodes, sheep. ResumoTimol é um monoterpeno e a acetilação deste composto pode reduzir a toxicidade e potencializar os seus efeitos biológicos. O objetivo deste trabalho foi avaliar o efeito do timol e acetato de timolila (AT) sobre ovos, larvas e adultos de Haemonchus contortus e suas alterações cuticulares, toxicidade aguda em camundongos e a eficácia sobre nematoides gastrintestinais de ovinos. Os resultados dos testes in vitro foram analisados por análise de variância (ANOVA) e comparados pelo testes de Tukey ou Bonferroni. A eficácia do teste de redução da contagem de ovos nas fezes (RCOF) foi calculada pelo programa BootStreet. No teste de inibição da eclosão de ovos (TEO), timol (0,5 mg/mL) e AT (4 mg/mL) inibiram a eclosão das larvas em 98% e 67,1%, respectivamente. Timol e AT (8 mg/mL) inibiram 100% do desenvolvimento larval. Timol e AT (800 µg/mL) reduziram a motilidade dos nematoides adultos, em 100% e 83,4%, respectivamente. O timol provocou alterações cuticulares nos nematoides adultos. No teste de toxicidade aguda, a DL50 do timol e AT foi de 1.350,9 mg/kg e 4.144,4 mg/kg, respectivamente. Timol e AT reduziram a contagem de ovos por gramas de fezes (OPG) dos ovinos em 59,8% e 76,2%, respectivamente. Nos testes in vitro timol apresentou atividade melhor anti-helmíntica do que AT. Entretanto, AT foi menos tóxico do que o timol e no teste in vivo apresentaram eficácia semelhante.

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“…Helminthosis caused by gastrointestinal nematodes, particularly Haemonchus contortus, is a prevalent and economically significant disease among domesticated animals (Perry and Randolph, 1999;Marie-Magdeleine et al, 2010). Gastrointestinal parasitic disease in small ruminants is characterized by anaemia, haemorrhagic gastroenteritis, hypoproteinaemia, sudden death, and chronic emaciation (Bethony et al, 2006;Botura et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

In vitro anthelmintic activity of Zanthoxylum simulans essential oil against Haemonchus contortus

Wang

Dai

et al. 2015

Veterinary Parasitology

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In vitro effects of Cassava (Manihot esculenta) leaf extracts on four development stages of Haemonchus contortus

Cited by 43 publications

References 38 publications

In VitroActivity ofPithecellobium DulceandLysiloma Acapulcensison Exogenous Development Stages of Sheep Gastrointestinal Strongyles

In VitroActivity ofPithecellobium DulceandLysiloma Acapulcensison Exogenous Development Stages of Sheep Gastrointestinal Strongyles

Anthelmintic effect of thymol and thymol acetate on sheep gastrointestinal nematodes and their toxicity in mice

In vitro anthelmintic activity of Zanthoxylum simulans essential oil against Haemonchus contortus

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