In vitro effects of calcium phosphate biomaterials on fibroblastic cell behavior

Abstract

The effect of synthetic granular hydroxyapatite (HAP) on cultured fibroblastic cells (L929, human bone and gingiva cells) was studied. Phagocytosis of HAP particles and resulting morphological cell changes were demonstrated by microscopic examinations. Cell counts and [3H]thymidine uptake indicated significant increases in cell proliferation and DNA synthesis. These results could account for some of the alterations of the fibroblast behavior induced by changes in intracellular levels of calcium ions released from the material.

Effect of hydroxyapatite sintering temperature on intracellular ionic concentrations of monocytes: A TEM-cryo-x-ray microanalysis study

“…This is in agreement with observations of Gregoire et al 2 Indeed, they showed that in MC3T3-E1 cells, the phagocytotic process of calcium phosphate powders stimulated protein synthesis. This is in agreement with Swan et al, 19 who have shown a correlation between surface area and secretion of cell factors.…”

Osteoblast precursor cell activity on HA surfaces of different treatments

“…39 It was observed that intracellular dissolution of calciumcontaining crystals greatly influence cell behavior. [40][41][42] The presence of HA particles and its intracellular solubilization were hypothesized to adversely affect calcium and phosphate homoeostatic mechanisms and to modify the mechanical regulators of DNA synthesis without any expression of cytotoxic effect. 43 Further studies are required to confirm this hypothesis.…”

In vivo calcium deposition on polyvinyl alcohol matrix used in hollow fiber cell macroencapsulation devices

“…These include the presence of a protein layer favoring protein-calcium complexes [43], the type of biomaterial including its physical characteristics [24,30] and cell death [25,30,35]. In addition to the deleterious effects of calcium particles on the structural integrity of biomaterials, hydroxyapatite was reported to alter the behavior of co-cultured cells [17,39] and to result in some cases in cell toxicity [15]. In order to avoid the appearance of calcifications, preventive strategies were attempted by modifying the material surface in conjunction with long-term controlled release of anticalcifying drug [7,31,35].…”