In Vitro Effects of C-Reactive Protein on Phagocytosis

Hokama, Y.; Coleman, Monroe; Riley, Ralph

doi:10.1128/jb.83.5.1017-1024.1962

Cited by 49 publications

(12 citation statements)

References 15 publications

(11 reference statements)

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“…This interaction of CRP to these compounds, especially C-polysaccharide, lecithin, and tissue polycations, has been shown to activate the human classic complement pathway and thus the release of physiologically active fragments, such as anaphylatoxin and chemotactic factors, of the classic C pathway (1,2,29,30,61,84,128-135). Similarly, the ability of CRP to bind various bacterial and tissue membranes probably contributes to the enhanced phagocytic activity of peripheral granulocytes in vitro, as shown by several investigators (39,53,136,137), although others (138) have shown no phagocytic enhancement using different test procedures. Binding of CRP to DNA with subsequent solubilization via complement activation has been suggested as one of the major functions of CRP (1 39).…”

Section: Characterization Of Crp: Physical Chemical and Biologicalmentioning

confidence: 84%

C‐Reactive protein: Current status and future perspectives

Hokama

Nakamura

1987

Clinical Laboratory Analysis

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This review encompasses all aspects of re-Reactive protein analysis relative to simplicity cent C-Reactive protein studies. Emphasis has and sensitivity. The responses of C-Reactive been in the areas of (1) the physical, chemical, protein in various diseases and its future prosand potential functions of C-Reactive protein; pects are also discussed in this review. and (2) the pass and recent procedures of C-

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Section: Characterization Of Crp: Physical Chemical and Biologicalmentioning

confidence: 84%

C‐Reactive protein: Current status and future perspectives

Hokama

Nakamura

1987

Clinical Laboratory Analysis

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“…Several earlier studies of CRP as a bacterial opsonin have produced conflicting results. Hokama et al (15) reported increased phagocytosis by PMN in whole blood of S. pneumoniae serotypes 2 and 27 and of Serratia marcescens pretreated with CRP. Williams and Quie (30) were unable to show any effect of CRP in the presence or absence of serum on killing of S. pneumoniae or other bacteria by PMN.…”

Section: Discussionmentioning

confidence: 99%

“…Injection of mice with CRP has been found to have a protective effect against infection with S. pneumoniae serotype 3 or 4 (20). Earlier reports of CRP opsonization of bacteria in vitro (15,17,30) have been conflicting.…”

mentioning

confidence: 99%

Effect of C-reactive protein on the complement-mediated stimulated of human neutrophils by Streptococcus pneumoniae serotypes 3 and 6

Mold¹,

Edwards²,

Gewürz³

1982

Infect Immun

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C-reactive protein (CRP) has long been known to appear in the sera of individuals with inflammatory processes, but its role in host defense against bacterial infection is unclear. We have recently demonstrated that CRP in the presence of the classical complement pathway markedly enhances the opsonization of Streptococcus pneumoniae serotype 27 by polymorphonuclear leukocytes (Edwards et al., J. Immunol. 128:2493-2496). In this report we have extended these studies to characterize the role of CRP in the opsonization of other S. pneumoniae serotypes. Two clinically important serotypes, 3 and 6, were tested along with the nonpathogenic rough strain R36a. All strains were found to bind radiolabeled CRP in the presence of calcium and to activate the classical complement pathway in normal human serum. However, the opsonophagocytic response of polymorphonuclear leukocytes to the strains, measured by chemiluminescence, was quite different. In contrast to the marked enhancement by CRP of the chemiluminescent response to serotype 27 in normal human serum, CRP had no effect on the opsonization of serotype 6 or R36a and inhibited opsonization of serotype 3 in normal serum. In serum from a hypogammaglobulinemic patient, CRP enhanced the lowered chemiluminescent response to serotype 3 and 6 organisms but did not restore the response to normal. The greater opsonic effect of CRP on serotype 27 may be related to the ability of CRP to bind to the capsule as well as to the cell wall of this serotype or to differences in the amount of CRP bound to the different strains.

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“…The precise role of increased synthesis, transport, and secretion of acute-phase reactants during infection remains to be determined, but may be purposeful (45). For example, it has been shown that C-reactive protein can stimulate the phagocytosis by leukocytes of living pathogenic and nonpathogenic bacteria (24,29,30). Potentially harmful proteinases released at sites of tissue damage can be complexed with serum alor a2-macroglobulin and rapidly eliminated from the circulation by fixed tissue phagocytes such as rabbit alveolar or peritoneal macrophages (12,16).…”

Section: Discussionmentioning

confidence: 99%

Synthesis, transport, and secretion of plasma proteins by the livers of control and Streptococcus pneumoniae-infected rats

Little¹

1978

Infect Immun

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The synthesis, intracellular transport, and secretion of plasma proteins by the liver was studied in both control and Streptococcus pneumoniae-infected rats. Rats were injected with [3H]leucine; at various time intervals, the components of the intracellular secretory system were isolated. The isolation and partial characterization of rough microsomes, smooth microsomes, and Golgi from both control and infected animals are described. After infection, the specific activity of the label in the homogenate and in all isolated cell fractions was significantly increased. In both control and infected animals, the kinetics of labeling suggested that the secretory pathway of the newly synthesized protein was from the rough endoplasmic reticulum to the smooth endoplasmic reticulum to the Golgi and finally to the circulation. Even though infected animals synthesized, transported, and secreted significantly more plasma protein, infection did not significantly alter the secretion time (time between injection of isotope and appearance of labeled protein in the circulation). It is concluded that, after S. pneumoniae infection, the liver still maintains its capability to perform the basic functions of protein synthesis, intracellular transport, and seretion and that newly synthesized plasma protein follows the same intracellular pathway in both control and S. pneumoniae-infected rats.

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In Vitro Effects of C-Reactive Protein on Phagocytosis

Cited by 49 publications

References 15 publications

C‐Reactive protein: Current status and future perspectives

C‐Reactive protein: Current status and future perspectives

Effect of C-reactive protein on the complement-mediated stimulated of human neutrophils by Streptococcus pneumoniae serotypes 3 and 6

Synthesis, transport, and secretion of plasma proteins by the livers of control and Streptococcus pneumoniae-infected rats

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