In Vitro Effects of Agriculture Pollutants on Microcrustacean and Fish Acid Phosphatases

Dantzger, Darlene Denise; Dantzger, Miriam; Jonsson, Cláudio Martín; Aoyama, Hiroshi

doi:10.1007/s11270-017-3570-7

Cited by 5 publications

(2 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 However, no inhibition was detected by this metal in the enzyme obtained from the microcrustacean Daphnia similis. 2 On the other hand, an increase in the Pseudokirchneriella subcapitata acid phosphatase activity occurred in the presence of Cu 2+ , due to one or more of the following factors: protection of the enzyme against thermal inactivation, increase in the enzyme-substrate affinity, and reduction of the activation energy, as detected from kinetic assays. 3 The high sensitivity of the enzyme crude extract to copper may be useful as a tool to detect this metal in natural waters and other environmental samples.…”

Section: Sewage Sludge/in Vitro Enzyme Activitymentioning

confidence: 99%

Effects of Pollutants and Role of the Soil in the Water Toxicity

Dantzger¹,

Aoyama²

2017

IJH

Self Cite

View full text Add to dashboard Cite

Section: Sewage Sludge/in Vitro Enzyme Activitymentioning

confidence: 99%

Effects of Pollutants and Role of the Soil in the Water Toxicity

Dantzger¹,

Aoyama²

2017

IJH

Self Cite

View full text Add to dashboard Cite

“…Another activity of ACP refers to the increased or reduced expression of this enzyme as a response to exposure to different types of pollutants. Therefore, the ACP could be potentially used as a pollution biomarker, particularly related to environmental contamination by heavy metals, microplastics and organophosphate pesticides (Patil et al, 2014;Dantzger et al, 2017;Zhao et al, 2021;Sang et al, 2023). In fact, highly sensitive fluorescent nanoprobes have been developed that function as an enzymatic amperimetric ACP-based sensor that can detect organophosphate insecticides even at low concentrations (below 2μg L -1 ) (Wang et al, 2022;Wang et al, 2017;Yamanaka, 2008).…”

Section: Introductionmentioning

confidence: 99%

In silico characterization of acid phosphatase in the functional genome of the amazon prawn

Sousa,

Lima,

Maciel

et al. 2024

Braz. J. Anim. Environ. Res.

View full text Add to dashboard Cite

The acid phosphatase (ACP) enzyme is responsible for the hydrolysis of phosphate monoesters under acidic conditions, being widely found in microorganisms, animals and plants. In crustaceans, the ACP plays a key role in the defense mechanism of distinct species. Furthermore, the expression of ACP is sensitive to pollution, being particularly related to the stress response by the exposure to heavy metals and organophosphate pesticides. However, most studies in crustaceans refer to the biochemical properties of ACP but little is known about its structural characterization. In the present work, we carried out in silico searches of ACP in the functional genome of Macrobrachium amazonicum, recognized as the most abundant freshwater prawn in Amazon. The ACP gene of M. amazonicum comprised a total of 3326 nucleotides (nt), including a complete open reading frame (ORF) of 1389 nt corresponding to the translation of 462 aminoacids. Signal peptide and intermembrane regions as well as conserved Pfam Pur_ac_phosph_N, Metallophos and Pfam Metallophos_C domains were present in the protein. In addition, we located 8 active sites, 25 homotrimeric and 15 homotetrameric interfaces, and 7 metal-binding sites. In general, we detected highly conserved sites shared among crustaceans. These data might be useful to provide effective biomarkers to assess the environmental quality and further biotechnological applications of ACP.

show abstract