In vitro Effectiveness of Commercial Bacteriophage Cocktails on Diverse Extended-Spectrum Beta-Lactamase Producing Escherichia coli Strains

Gündoğdu, Aycan; Bolkvadze, D.; Kılıç, Hüseyin

doi:10.3389/fmicb.2016.01761

Cited by 20 publications

(16 citation statements)

References 40 publications

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“…The combination of phages with adjuvant components is a currently understudied topic, with great focus being placed on the combination of phages and conventional antibiotics (Chaudhry et al 2017;Oechslin et al 2017;Scanlan et al 2017) and purified phage cocktails (Gundogdu et al 2016;Regeimbal et al 2016;Yen et al 2017). However, in order to further develop phages as antimicrobial agents, it is necessary to investigate the impact of more complex formulations which include additional active and excipient components (Abedon 2017).…”

Section: Combined Activity Of Bacteriophages and Bulk Plant Extractsmentioning

confidence: 99%

In vitroactivity of a combination of bacteriophages and antimicrobial plant extracts

Pimchan

Cooper

Eumkeb

et al. 2018

Lett Appl Microbiol

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Significance and Impact of Study: This preliminary study provides insights into the potential combination of bacteriophages and antimicrobial plant bulk extracts to target bacterial pathogens. It is to our knowledge the first time in which virulent bacteriophages have been combined with antimicrobial plant extracts. AbstractThe continuing threat of antimicrobial resistance presents a considerable challenge to researchers to develop novel strategies ensuring that bacterial infections remain treatable. Many plant extracts have been shown to have antibacterial properties and could potentially be combined with other antibacterial agents to create more effective formulations. In this study, the antibacterial activity of three plant extracts and virulent bacteriophages have been assessed as individual components and in combination. When assessed with a modified suspension test, these plant extracts also exhibit antiviral activity at bacterial inhibitory concentrations. Hence, to investigate any potential additive effects between the extracts and virulent phages, the extracts were tested at subantiviral concentrations. Phages alone and in combination with plant extracts significantly reduced (P < 0Á05) the bacterial concentration compared to untreated and extract treated controls up to 6 h (2-3log 10 ), but this reduction did not extend to 24 h. In most cases, the phage and extract combinations did not significantly reduce bacterial content compared to phages alone. Additionally, there was little impact on the ability of the phages to reproduce within their bacterial hosts. To our knowledge, this study represents the first of its kind, in which antimicrobial plant extracts have been combined with virulent phages and has highlighted the necessity for plant extracts to be functionally characterized prior to the design of combinatorial therapies.

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Section: Combined Activity Of Bacteriophages and Bulk Plant Extractsmentioning

confidence: 99%

In vitroactivity of a combination of bacteriophages and antimicrobial plant extracts

Pimchan

Cooper

Eumkeb

et al. 2018

Lett Appl Microbiol

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show abstract

“…In terms of commercially produced phage stocks, main constituents, and host range of them have been disclosed before [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Staphylococcal bacteriophage containing Sb-1 ( Myoviridae ), active against S. aureus [ 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

“…Pyo bacteriophage containing Sb-1 ( Myoviridae ) and ISP ( Myoviridae ) active against Staphylococcus spp., Streptococcus spp., Proteus spp., P. aeruginosa , and E. coli [ 32 , 33 ]. Ses active against Staphylococcus spp., Streptococcus spp., enteropathogenic E. coli [ 34 ]. Fersisi containing B1 ( Myoviridae ) and JA1 ( Myoviridae ) active against Staphylococcus spp., Streptococcus spp.…”

Section: Methodsmentioning

confidence: 99%

“…[ 35 , 36 ]. Enko active against Salmonella spp., Shigella spp., Staphylococcus spp., enteropathogenic E. coli [ 34 ]. Intesti containing D1-D18, F1-F4 and Proteus phage ( Myoviridae , Siphoviridae , Podoviridae ) [ 37 ], active against Shigella spp., Salmonella spp., E.coli , Proteus spp., Staphylococcus spp., P.aeruginosa , Enterococcus spp.…”

Section: Methodsmentioning

confidence: 99%

“…Intesti containing D1-D18, F1-F4 and Proteus phage ( Myoviridae , Siphoviridae , Podoviridae ) [ 37 ], active against Shigella spp., Salmonella spp., E.coli , Proteus spp., Staphylococcus spp., P.aeruginosa , Enterococcus spp. [ 32 , 34 ]. Pyobacteriophag containing fRuSau02 ( Myoviridae ) and SCH1 (Podoviridae) active against Staphylococcus spp., Streptococcus spp., Enterococcus spp., Proteus spp., Klebsiella spp., P. aeruginosa , and E.coli [ 33 , 36 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

S. aureus Colonization, Biofilm Production, and Phage Susceptibility in Peritoneal Dialysis Patients

et al. 2020

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Peritonitis caused by Staphylococcusaureus is of major importance in peritoneal dialysis (PD) patients due to its great virulence profile and biofilm formation ability. Bacteriophages are a potential tool to treat peritonitis resulting from biofilm-associated infections. We screened S. aureus colonization in 71 PD patients from the nasal cavity, groin, and PD exit-site regions and analyzed clinical outcomes in these patients. We performed biofilm-formation testing of different strains and compared the isolates of one patient to detect phenotypic differences in S. aureus. Phage cocktails were used to detect S. aureus in vitro susceptibility. An adaptation procedure was performed in cases of bacterial resistance. Around 30% of PD patients (n = 21) were found to be S. aureus carriers; from these, a total of 34 S. aureus strains were isolated, of which 61.8% (n = 21) produced a strong biofilm. Phenotypic differences in strain biofilm production were detected in eight patients out of ten. All strains were sensitive to commonly used antibiotics. Broadly positive phage lytic activity (100%) was observed in six cocktails out of seven, and bacterial resistance towards phages was overcome using adaptation. Overall phages showed a promising in vitro effect in biofilm-forming S. aureus strains.

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Bacteriophages as a potential substitute for antibiotics: A comprehensive review

Kushwaha,

Sahu,

Yadav

et al. 2024

Cell Biochemistry & Function

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Over the years, the administration of antibiotics for the purpose of addressing bacterial infections has become increasingly challenging due to the increased prevalence of antimicrobial resistance exhibited by various strains of bacteria. Multidrug‐resistant (MDR) bacterial species are rising due to the unavailability of novel antibiotics, leading to higher mortality rates. With these conditions, there is a need for alternatives in which phage therapy has made promising results. Phage‐derived endolysins, phage cocktails, and bioengineered phages are effective and have antimicrobial properties against MDR and extensively drug‐resistant strains. Despite these, it has been observed that phages can give antimicrobial activity to more than one bacterial species. Thus, phage cocktail against resistant strains provides broad spectrum treatment and magnitude of effectivity, which is many folds higher than antibiotics. Many commercially available endolysins such as Staphefekt SA.100, Exebacase (CF‐301), and N‐Rephasin®SAL200 are used in biofilm penetration and treating plant diseases. The role of CMP1 phage endolysin in transgenic tomato plants in preventing Clavibacter michiganensis infection and the effectiveness of phage in protecting Atlantic salmon from vibriosis have been reported. Furthermore, phage‐derived endolysin therapy, such as TSPphg phage exogenous treatment, can aid in disrupting cell walls, leading to bacterial cell lysis. As animals in aquaculture and slaughterhouses are highly susceptible to bacterial infections, effective phage therapy instead of antibiotics can help treat poultry animals, preserve them, and facilitate disease‐free trade. Using bioengineered phages and phage cocktails enhances the effectiveness by providing a broad spectrum of phages and target specificity. Research is currently being conducted on clinical trials to confirm the efficacy of engineered phages and phage cocktails in humans. Although obtaining commercial approval may be time‐consuming, it will be beneficial in the postantibiotic era. This review provides an overview of the significance of phage therapy as a potential alternative to antibiotics in combating resistant bacterial strains and its application to various fields and emphasizes the importance of safeguarding and ensuring treatment efficacy.

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In vitro Effectiveness of Commercial Bacteriophage Cocktails on Diverse Extended-Spectrum Beta-Lactamase Producing Escherichia coli Strains

Cited by 20 publications

References 40 publications

In vitroactivity of a combination of bacteriophages and antimicrobial plant extracts

In vitroactivity of a combination of bacteriophages and antimicrobial plant extracts

S. aureus Colonization, Biofilm Production, and Phage Susceptibility in Peritoneal Dialysis Patients

Bacteriophages as a potential substitute for antibiotics: A comprehensive review

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