In Vitro Effect Of Sodium Azide And Ethyl Methanesulphonate On Secondary Metabolite Production In Andrographis paniculata (Burm.F.) Nees

Abstract

Comparative effects of 0.03% of EMS(Ethyl methanesulphonate) and 0.03% SA (Sodium azide) for 5 hours on secondary metabolite production in Andrographis paniculata were conducted in this study. Calli derived from leaf explants inoculated on Murashige and Skoog's (MS) medium supplemented with 2 mg/l NAA, 1 mg/l Kinetin and 50 mg/l Phenylalanine. Treatment of 0.03% SA for 5 hours resulted in 7 fold increase in andrographolide production when compared to the control and the same concentration of EMS for 5 hours treatment. The study also showed that 0.03% SA for 5 hours treated samples showed enhanced production in the fresh weight, dry weight of calli, callus induction frequency and andrographolide production compared to the control.