In vitro effect of seed bio-priming techniques on seed germination and seedling vigour of few vegetable crops

Abstract

Bio-priming can also be viewed as a new technique of seed treatment using biological agents to stimulate germination of seed and growth of the plant and further protecting the seed from soil-and seed-borne pathogens. The present investigation was carried out in vitro conditions on seed germination and seedling vigour of few vegetable crops viz. tomato (Solanum lycopersicum L.), brinjal (Solanum melongena L.), onion (Allium cepa L.) and chilli (Capsicum annuum L.), during 2015 and 2016. The treatments comprised viz. T1: Non primed seeds (Control), T2: Seed treatment with Carbendazim 2.5g/kg seed, T3: Hydro-priming for 6 hrs, T4: Hydro-priming for 12 hrs, T5: Hydro-priming for 18 hrs, T6: Biopriming with Trichoderma viride for 6 hrs, T7: Biopriming with T.viride for 12 hrs, T8: Biopriming with T.viride for 18 hrs, T9: Biopriming with Trichoderma harzianum for 6 hrs, T10: Biopriming with T. harzianum for 12 hrs, T11: Biopriming with T. harzianum for 18 hrs, T12: Biopriming with Pseudomonas fluorescens for 6 hrs, T13: Biopriming with P. fluorescens for 12 hrs and T14: Biopriming with P. fluorescens for 18 hrs. The results revealed that maxiumum germination percentage (92.92, 90.77,83.00 and 86.33), seedling length (32.38 cm, 29.35 cm, 31.75 and 31.60 cm), seedling fresh weight (2.07 g, 4.01 g, 3.05 g and 2.04 g), seedling dry weight (0.42 g, 0.86 g, 0.62 g and 0.42 g) and seedling vigour index (3008.11, 2664.00, 2635.00 and 2728.00) were recorded in T10 (bio priming with T. harzianum for 12 hrs) in tomato (S. lycopersicum L.), brinjal (S. melongena L.), onion (A. cepa L.) and chilli (C. annuum L.), respectively. Thus, it indicated that priming of seeds of these crops with T. harzianum/P. fluorescens/ T. viride for 12 hrs was very effective with respect to their vegetative growth along with the quality yield.

Characterization and selection of endophytic actinobacteria for growth and disease management of Tea (Camellia sinensis L.)

“…Many bacteria such as Pseudomonas sp., Bacillus sp., and Azotobacter sp. with beneficial traits have been well documented as bioinoculant or seed bio-priming agents and observed to improve seed germination and protect the plant from biotic stress ( Jaiman et al., 2020 ; Mitra et al., 2021 ). In a report, biopriming soyabean with Streptomyces sp.…”

Biopriming of cayenne seed (Capsicum frutescens L.) using Indole Acetic Acid (IAA) - producing fluorescent pseudomonads to increase germination and growth of seeds

“…As stated by [28], 11 isolates of Pseudomonas isolated from the rhizosphere produced IAA with concentrations between 10.2 to 31.2 ppm, and could increase germination and wet weight of chickpea seedlings. Jaiman et al, [29] stated that the increase in plant vegetative growth parameters was associated with the biopriming role of Pseudomonas fluorescens and Trichoderma harzianum in fixing nitrogen, producing growth hormones such as gibberellins, cytokinin, and auxins that can stimulate nutrient absorption and play a role in the photosynthesis process.…”