In Vitro Effect of photodynamic therapy on Aggregatibacter actinomycetemcomitans and Streptococcus sanguinis

Mattiello, Fabiano Dalla Lana; Coelho, Alan Augusto Kalife; Martins, Odair Pimentel; Mattiello, Rodrigo Dalla Lana; Júnior, José Peixoto Ferrão

doi:10.1590/s0103-64402011000500009

Cited by 34 publications

(29 citation statements)

References 18 publications

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“…We also aimed to compare the efficacy of this method with the use of each component alone, and we found that simultaneous application of curcumin and LED laser inhibited the growth of A. actinomycetemcomitans more efficiently than using curcumin or LED laser individually. Another survey by Mattiello et al 22 assessed the effectiveness of 0.01% toluidine blue accompanied by diode laser of Aluminum Gallium Indium Phosphorus (AlGaInP), on A. actinomycetemcomitans. They also found a positive effect of PDT on this bacterium, which was compatible with our results.…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

et al. 2016

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IntroductionPeriodontal diseases are referred to as inflammatory processes in periodontal tissues in response to the aggregation of bacteria on the teeth. The aggregation rarely leads to evident infection, but the inflammatory response caused in the gingival tissue is responsible for the gradual loss of dental collagen's attachment to the alveolar bone. Left overlooked, this phenomenon might end in increased dental mobility and eventually edentulism.1 Production of destructive metabolites by gram negative and positive bacteria of the microbial plaque in the oral cavity causes gingivitis, which can play a key role in the progression of inflammation to periodontal diseases. 2Aggregatibacter actinomycetemcomitans is an immobile microaerophilic, facultative anaerobic, gram negative coccoid rod, 3 strongly associated with pathogenesis of periodontal diseases, particularly aggressive generalized periodontitis.4 Some of this bacterium's membranous proteins play an important role in attachment to and penetration in tissue cells.5 Other virulence factors secreted by this bacterium include an agent that inhibits the reproduction of fibroblasts and a leukotoxin that kills the leukocytes. 6 Despite the many complications caused by periodontitis, no treatment protocol is known to be able to fully control this disease. Currently, a combination of mechanical treatments and systemic antibiotics are used as treatment 7 ; however, periodontal pathogens are not completely removed by the mechanical removal of the biofilm; and there are problems associated with the use of antibiotics, Abstract Introduction: Considering the importance of prevention in periodontal diseases and the important role of Aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate (CHX), curcumin and light-emitting diode (LED) laser, on this bacterium. Methods: Antimicrobial activity of curcumin (5 mg/ml), CHX (2%), LED (120 J/cm 2 ) and LED + curcumin (120 J/cm 2 + 2.5 mg/ml) against A. actinomycetemcomitans were tested in vitro, using micro-broth dilution test. One-way analysis of variance (ANOVA) and Tukey's HSD tests served for statistical analysis. Results: Regarding the minimum inhibitory concentration (MIC), CHX had a significantly lower MIC than curcumin (P < 0.05). Sorted out by bacterial growth from lowest to highest, were CHX, LED + curcumin, curcumin, and LED groups. All the differences were found to be statistically significant (P < 0.05) except for the LED group. Conclusion: We conclude that curcumin is an effective substance in preventing the growth of A. actinomycetemcomitans, whose impact is reinforced when used simultaneously with photodynamic therapy (PDT).

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Section: Discussionmentioning

confidence: 99%

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

et al. 2016

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“…Apresenta um baixo custo para sua produção e baixo risco para olhos desprotegidos. Ainda, está sendo empregado na TFA devido ao seu alto custo-benefício [20,21].…”

Section: Análise Da Efetividade Da Tfaunclassified

Efeitos da Terapia Fotodinâmica Antimicrobiana sobre Microrganismos Cariogênicos: Estudo Preliminar

Paschoal¹,

Silva²,

Silva³

et al. 2014

Pesqui. bras. odontopediatria clín. integr.

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ResumoObjetivo: Investigar a sensibilidade de uma cepa laboratorial de Streptococcus mutans e dois isolados clínicos do grupo estreptococos à terapia fotodinâmica antimicrobiana (TFA). Método: Azul de toluidina (TBO) e diodos emissores de luz (LEDs) no comprimento de onda vermelho (600 -700 nm) foram utilizados objetivando a morte de células planctônicas de uma cepa de referência de S. mutans (ATCC 25175) e dois isolados clínicos de estreptococos. Os microrganismos foram cultivados em infusão cérebro coração (BHI) em condições de microaerofilia a 37oC por 24 horas. Subsequentemente, suspensões foram transferidas para placas de 96 poços e tratadas com a combinação de quatro concentrações de TBO e quatro doses de luz. Grupos controle não foram tratados ou tratados com TBO e LED isoladamente. A efetividade da TFA foi avaliada por meio de plaqueamento e microscopia de varredura confocal a laser (MVCL). Resultados: O efeito antimicrobiano da TFA foi aumentado pela presença do TBO e pelo aumento das doses de luz. Resultado antibacteriano similar foi observado para os dois isolados clínicos (2,5 µg.mL-1 TBO a 24 J.cm-2 LED), em que a combinação de 0,25 µg.mL-1 TBO e 36 J.cm-2 LED foi efetiva para inativação da cepa de referência de S. mutans. Conclusão: Embora ligeiras variações foram observadas entre as cepas, TFA foi efetiva na fotoinativação de células planctônicas dos microrganismos estudados.Descritores: Cárie dentária. Fotoquimioterapia. Stretococcus mutans. Diversos agentes químicos são usados concomitantemente à escovação objetivando a prevenção da cárie dentária [7]. Terapia fotodinâmica antimicrobiana (TFA) é uma abordagem relativamente nova para o controle de patógenos envolvidos com a doença [8,9]. O método consiste na fotoativação de fotossensibilizadores ou corantes por um comprimento de onda especifico. Radicais livres como oxigênio singleto, superóxidos e hidroxilas são produzidos durante a irradiação e esses produtos tóxicos são usados no metabolismo bacteriano com consequente morte celular [10,11].A efetividade da TFA na inativação de S. mutans tem sido demonstrada por estudos prévios [8,9]. Cepas clínicas são frequentemente mais resistentes a antimicrobianos que cepas padrão ou laboratoriais devido a trocas genéticas entre microrganismos presentes na cavidade bucal [12][13].Bactérias podem desenvolver resistência por meio da aquisição de material genético novo de outros organismos resistentes, ocorrendo entre cepas de mesma ou distinta espécies ou gêneros por troca genética (ex. conjugação, transdução e transformação) [14]. Baseado no fato de que cárie dentaria é capaz de destruir estruturas mineralizadas em qualquer estágio da vida, crianças são mais afetadas por essa doença devido a imaturidade de sua destreza manual e, como consequência, não atingindo um nível ótimo de remoção de placa dentária ou biofilme [15]. Embora um estudo recente tenha provado a resistência de isolados clínicos à TFA [13], nenhum estudo prévio focou-se no efeito da TFA sobre isolados clínicos de microrganismos cariogênicos ...

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“…3 Light and drug are nontoxic by themselves; hence, only cells bound by PS and receiving light are affected by ''photodynamic action.'' 3 PACT treatments of bacteria including Streptococcus mutans, 4,5 Streptococcus sanguinis, 6 Porphyromonas gingivalis 7 and Actinobacillus actinomycetemcomitans 6 have shown positive outcomes. Toluidine blue has been shown to have bactericidal effects against gram-positive and gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Streptococcus MutansPhotoinactivation by Combination of Short Exposure of a Broad-Spectrum Visible Light and Low Concentrations of Photosensitizers

Paschoal

Pinto

Lin

et al. 2014

Photomedicine and Laser Surgery

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Objective: Investigate the photodynamic antimicrobial effect by the combination of a novel noncoherent broad spectrum visible light and low concentrations of curcumin and toluidine blue over suspensions of Streptococcus mutans. Background data: Long illumination times to activate photosensitizers (PS) and the use of high concentrations of these drugs in photodynamic antimicrobial chemotherapy (PACT) are limitations of its application as an antimicrobial technology in dental practice. Materials and methods: Planktonic suspensions of S. mutans were standardized and submitted to PACT treatment at low concentrations of curcumin (C) (0.075; 0.75 and 7.5 lM) and toluidine blue (T) (0.25; 2.5 and 25 lM) exposed to 42 J/cm 2 (12.2 sec; set power: 3.930 mW) of a white light (WL) (output wavelength range: 400-700 nm; beam diameter: 12 mm) (C + WL + and T + WL + , PACT groups; incubation time, C: 60 sec; T: 5 min); isolated effect of both C (C + WL -) and T concentrations (T + WL -); effect of light source (C -WL + and T -WL + ) and suspensions neither submitted to PS nor to light-emitting diode (LED) illumination (control groups, C -WL -and T -WL -). Aliquots of each group were diluted and cultured on blood agar plates and the number of colony-forming units (CFU)/mL was recorded, transformed into log 10 and analyzed by ANOVA and Tukey's test at a cutoff value at 0.05. Results: The groups submitted to PACT presented a bacterial reduction value of > 5-log 10 to both tested PS in comparison with control groups ( p < 0.05). PS or light source used alone demonstrated no antimicrobial effect on the number of viable bacterial counts. Conclusions: The combination of a novel noncoherent light at short illumination exposure time with low concentrations of studied PS achieved a lethal photoinactivation of S. mutans, and can be considered an effective antimicrobial in vitro approach for reducing the number of microorganisms involved with the dental caries process.

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In Vitro Effect of photodynamic therapy on Aggregatibacter actinomycetemcomitans and Streptococcus sanguinis

Cited by 34 publications

References 18 publications

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

Efeitos da Terapia Fotodinâmica Antimicrobiana sobre Microrganismos Cariogênicos: Estudo Preliminar

Streptococcus MutansPhotoinactivation by Combination of Short Exposure of a Broad-Spectrum Visible Light and Low Concentrations of Photosensitizers

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