In vitro Effect of LC90 of albendazole and Allium sativum water extract on the fine structure of Capillaria sp. (Capillaridae Nematoda).

Abstract

Fish parasites are of economic and health importance where they cause serious problems in commercial fish farms and it can be transferred to human and animals. Capillaria species are nematodes of serious fish diseases which may be transferred to human.The present study was conducted to detect the in vitro effect of sublethal concentration (LC 90) of albendazole(ABZ) and Allium sativum (garlic) cloves water extract on ultra structure of Capillaria sp. collected from fresh water catfishes, Bagrus docmac and B. bayad using SEM and TEM. The morality rates of the adult Capillaria sp. in vitro exposed to different concentrations of albendazole and Allium sativum water extract was recorded after 24 hours exposure time. A. sativum water extract had higher effect than albendazole where lower concentration of A. sativum extract (18 x10 3 ppm) gave maximum mortality rate (100%) , 24 hours post exposure. SEM studies of the cuticle of adult Capillaria sp. in vitro treated with LC 90 (17.161 x10 3 ppm) of A. sativum water extract for 24 hours revealed that it induced severe changes in the cuticle integrity more than those treated with concentration LC 90 (5.543 x 10 6 ppm) of albendazole. TEM studies revealed deformation of the cuticle, hypodermis, muscles and digestive system in worms treated with LC 90 (17.161 x10 3 ppm) of Allium sativum water extract.

Parasites of Public Health Importance in Nile and Cultured Fish in El-Minya Governorate

“…in the present work was not detected in other species of examined fish (Bolty, Catfish and Carp fish). This result did not agree with previous studies of Abdel-Rahman et al (2019), who detected it in Bolty and with Khalil et al (2016) who detected it in Catfish.…”

“…and its larvae were found only in Bagrus Bajad. Capillaria spp was detected previously in the same fish species (Bagrus Bajad) by Mohamed et al (2003), Khalil et al (2014), Khalil et al (2016) also Okpasuo et al (2016).…”

The Effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum sativum L., Satureja hortensis L. and Calendula officinalis L. on the Embryogenesis of Ascaris suum Eggs during an In Vitro Experimental Study

“…Moreover, the aqueous and alcoholic extracts of Allium sativum L. proved a good anthelmintic effect on Capilaria spp. in fish and sheep [27,28]. In the present study, the ethanolic garlic extract was the most effective extract at all concentrations used.…”