In Vitro Effect of Escherichia Coli on Human Sperm Acrosome Reaction

Elmulla, KhaledF; Köhn, F.‐M.; Dandal, M; Beheiry, Adel Hassan El; Schiefer, Hans-Gerd; Weidner, W.; Schill, W.‐B.

doi:10.3109/01485019608988505

Cited by 35 publications

(17 citation statements)

References 17 publications

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“…Bacteria including Ureaplasma urealyticum and Mycoplasma hominis have been demonstrated to have a negative effect on ionophore-induced acrosomal reaction of human spermatozoa in vitro [26, 27]. El-Mulla et al also studied the effect of E. coli on acrosomal reaction of spermatozoa [28]. It was shown that the inducibility of the acrosome reaction was significantly lower in semen samples pretreated with E. coli than in the control samples.…”

Section: Discussionmentioning

confidence: 99%

Human Sperm Interaction withStaphylococcus aureus: A Molecular Approach

Gupta

Prabha

2012

Journal of Pathogens

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Sperm immobilization factor (SIF) causing 100% immobilization of spermatozoa isolated from Staphylococcus aureus when characterized using LC-MS (Liquid chromatography-mass spectrometry) showed that this 20 kDa protein had peptide sequence similarity with hsp-70 protein. It was found to completely (100%) inhibit Mg++ ATPase activity of spermatozoa at concentration of 100 μg mL−1. Sperm samples treated with SIF also showed reduction in calcium ionophore-induced acrosome reaction as compared to control samples (treated with calcium ionophore alone). Binding studies of FITC labelled SIF with spermatozoa using fluorescent microscopy showed binding of SIF to the surface of spermatozoa indicating the presence of SIF binding receptor. The receptor was extracted by 3M NaCl and purified by gel permeation chromatography. Characterization of the receptor by MALDI-TOF (Matrix-assisted laser desorption ionization-time of flight) indicated that the receptor shared sequence similarity with MHC class II antigen. A calorimetric study showed that the receptor moiety on spermatozoa was specific for the purified ligand as binding of the receptor to ligand was enthalpically (−11.9 kJ mole−1) as well as entropically (21.53 J mole−1 K−1) favored resulting in the Gibb's free energy of −18.57 kJ mole−1.

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Section: Discussionmentioning

confidence: 99%

Human Sperm Interaction withStaphylococcus aureus: A Molecular Approach

Gupta

Prabha

2012

Journal of Pathogens

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“…The change in acrosomal cap is mainly due to sperm aging or injury (Saacke and Marshall 1968). Presence of micro‐organisms, especially the bacteria in the ejaculates can affect fertilization directly (Morrell 2006) by inducing acrosome reaction (El‐Mulla et al. 1996).…”

Section: Discussionmentioning

confidence: 99%

Effect of Antibiotics in Extender on Bacterial and Spermatozoal Quality of Cooled Buffalo (Bubalus bubalis) Bull Semen

Akhter

Ansari

Andrabi

et al. 2007

Reprod Domestic Animals

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The present study was designed to study the effect of traditional antibiotic combination (streptomycin and penicillin; SP) and relatively modern combination of antibiotics (gentamycin, tylosin, lincomycin and spectinomycin; GTLS) in extender on bacterial control and spermatozoal quality of liquid buffalo bull semen stored at 5 degrees C. Semen collected from Nili-Ravi buffalo bulls (n = 10) was diluted with skim milk extender containing either SP (streptomycin 1000 microg/ml and penicillin 1000 IU/ml), GTLS (gentamycin 500 microg/ml, tylosin 100 microg/ml, lincomycin 300 microg/ml and spectinomycin 600 microg/ml) or negative control with no antibiotics (NA). Liquid semen was stored at 5 degrees C for 5 days. Aerobic bacteria isolated from buffalo semen were Pseudomonas aeruginosa and Staphylococcus aureus. The only facultative anaerobic bacterium isolated was Klebsiella pneumoniae. In vitro antibiotic sensitivity test revealed that Ps. aeruginosa and Staph. aureus were susceptible to gentamycin. Staphylococcus aureus and K. pneumoniae were susceptible to tylosin and linco-spectinomycin. Total aerobic bacterial count was significantly lower in semen samples treated with GTLS than those of SP on third and fifth day of storage at 5 degrees C. There was no difference (p > 0.05) in sperm motility, longevity and plasma membrane integrity (PMI) in extender containing SP or GTLS combination until the third day of storage at 5 degrees C. On fifth day of storage sperm motility, longevity and PMI was significantly better in extender containing SP compared with GTLS and NA. Intact acrosomes, and sperm head, mid piece and tail abnormalities remained similar (p > 0.05) because of antibiotics up to 5 days of storage. In conclusion, GTLS is more capable than SP for bacterial control of buffalo bull semen. Moreover, GTLS and SP are equally efficient in preserving spermatozoal quality of extended buffalo bull semen for 3 days at 5 degrees C.

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“…However, Hasan et al (2001) reported higher sperm acrosomal integrity in semen cryopreserved in extenders having antibiotics compared to that without antibiotics. Bacteria if present in the semen can adhere to the spermatozoa and induce premature acrosome reaction (El-Mulla et al 1996), therefore, this difference may be attributed to reproductive health/hygienic conditions of bulls or difference of technique. The present study evaluated live sperm with intact acrosome, while, the previous studies (Hasan et al 2001;Akhter et al 2008) reported normal apical ridge.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, storage media is supportive for microbial growth. Fertilizing capacity of spermatozoa can be directly affected by bacteria (Morrell 2006) that can impair the sperm motility (Panangala et al 1981), have the ability to adhere with spermatozoa (Diemer et al 1996), and can induce the acrosome reaction (El-Mulla et al 1996). During improper handling and processing of semen, as the bacteria proliferate, they release various metabolites that can change the seminal characteristics, such as pH, metabolic products, or free radicals (Das et al 1994;Dhami & Sahni 1994;Althouse et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Comparison of traditional antibiotic streptomycin with neomycin, polymyxin B, or colistin in extender for buffalo (Bubalus bubalis) bull spermatozoa

Qadeer¹,

Batool²,

Mehboob³

et al. 2013

Journal of Applied Animal Research

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This study was designed to compare the effect of traditional antibiotics streptomycinÁpenicillin (SP) with neomycinÁpenicillin (NP), polymyxinÁpenicillin (PP), and colistinÁpenicillin (CP) in extender against the postthaw quality and bacterial count of buffalo (Bubalus bubalis) bull spermatozoa. Qualifying semen ejaculates (n 018) from three Nili-Ravi buffalo bulls were splits into five aliquots and diluted (50 )10 (6 spermatozoa/ml; 378C) in extender containing SP, NP, PP, CP, or without antibiotics (control). The diluted semen was cooled to 48C in 2 hours and equilibrated for 4 hours. Cooled semen was filled in 0.5 ml French straws at 48C, kept over liquid nitrogen vapors (5 cm) for 10 minutes and then plunged in the liquid nitrogen for storage. For the assessment of post-thaw semen quality (motility, plasma membrane integrity (PMI), sperm viability) and total aerobic bacterial count (TABC), thawing was performed at 378C for 30 seconds. Sperm motility, PMI, sperm viability, and TABC remained similar (P!0.05) in all experimental extenders containing SP, NP, PP, CP, and extender without antibiotics. It is suggested that neomycin, polymyxin, or colistin in combination with penicillin did not deteriorate semen quality and may be suggested to replace streptomycin in traditional antibiotic combination for cryopreservation of Nilli-Ravi buffalo bull spermatozoa.

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In Vitro Effect of Escherichia Coli on Human Sperm Acrosome Reaction

Cited by 35 publications

References 17 publications

Human Sperm Interaction withStaphylococcus aureus: A Molecular Approach

Human Sperm Interaction withStaphylococcus aureus: A Molecular Approach

Effect of Antibiotics in Extender on Bacterial and Spermatozoal Quality of Cooled Buffalo (Bubalus bubalis) Bull Semen

Comparison of traditional antibiotic streptomycin with neomycin, polymyxin B, or colistin in extender for buffalo (Bubalus bubalis) bull spermatozoa

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