In vitro effect of classical swine fever virus 
on a porcine aortic endothelial cell line

Campos, Emilia; Revilla, Concepción; Chamorro, Sonia; Álvarez, Belén; Ezquerra, Ángel; Domı́nguez, Javier; Alonso, Fernando

doi:10.1051/vetres:2004041

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…Other in vitro investigations showed that CSFV also disrupted secretion of IFN-α and proinflammatory cytokines in porcine aortic and kidney endothelial cell lines [7,11]. It is to emphasize that CSFV-infected mo-DC were able to mature only when TNF-α and IFN-α were added into culture medium [13].…”

Section: Discussionmentioning

confidence: 94%

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

et al. 2007

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-Classical swine fever virus (CSFV) compromises the host immune system, causing indirect leucopoenia and disruption of in vitro T cell stimulation capacity. In order to explore the potential role of dendritic cells (DC) in such phenomena, the activation of conventional DC (cDC) and plasmacytoid DC (pDC) in blood and secondary lymphoid organs of infected pigs was investigated in the early time course post-inoculation (pi), together with viral components dissemination and cytokine production in serum. Whereas CD11R1 + CD172a + cDC frequencies were markedly reduced in blood and spleen, analysis of CD4 + CD172a + pDC numbers revealed a rapid turn-over of this DC subset in tissues pi. Both subsets matured and were activated after infection, as demonstrated by down-regulation of CD1a, up-regulation of the co-stimulation molecule CD80/86 and expression of cytokines. cDC essentially expressed tumor necrosis factor alpha (TNF-α) and interleukin (IL)-10, whereas pDC produced alpha interferon (IFN-α) and IL-12. IFN-α and TNF-α productions revealed an enhancement of innate anti-viral immune responses. Detection of antigen activated B lymphocytes in tonsil T-cell areas at 72 h pi, subsequently to the transient translocation of the viral E2 protein within germinal centres at 48 h pi, indicates the initiation of humoral response. This response was also evidenced by an important IL-10 production in serum one week pi. IL-12 expression in organs, as well as transient detection of IL-18 and IFN-γ in serum, reflected the initiation of cellular immune responses. However, the uncommonly high levels of TNF-α and IFN-α produced by DC and measured in serum early post-infection, together with IL-10 expression in spleen, could play a role in the disruption of immune system cells, either inducing apoptosis or impairing DC functionalities themselves.antigen presenting cells / secondary lymphoid organs / interferon / E2 antigen / pestivirus

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Section: Discussionmentioning

confidence: 94%

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

et al. 2007

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“…CSFV infection induced a functional decrease in the pro-coagulant activity of the ECs, which were highly susceptible to CSFV infection. 33 the inhibitory effects of APS on porcine viruses infection have verified 34 and APS has been observed to regulate the levels of immunomodulating compounds in many different cell types. 35,36 The manifestation of adhesion molecules, including P-selectin and E-selectin, was inhibited by APS and persisted while the ECs were stimulated with CSFV, which could promote the expression of both P-and E-selectin ( Figure 2AB).…”

Section: Discussionmentioning

confidence: 89%

Peer Review #1 of "Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFV in vitro (v0.2)"

2017

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Background: Astragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown. Methods: The virus was titrated using an indirect immune biotin enzyme standard method to confirm that porcine ECs were susceptible to CSFV infection and to determine the TCID50 of CSFV (C-strain). Porcine ECs were cultured with CSFV in the presence of APS. Relative quantitative PCR was used to assess the mRNA expression of factors that influence EC adhesion and immunity. Results: The expression of adhesion factors mRNA increased following stimulation with CSFV; this effect was inhibited by pre-exposing the cells to APS. In addition, the expression of growth factors and some immune factors increased after infection with CSFV; this increase in tissue factor (TF), transforming growth factor (TGF-β), and interleukin-8 (IL-8) could be inhibited by the addition of APS. The immune response mediated by Toll-like receptor 4 (TLR4) in ECs may be unregulated by CSFV as it was also inhibited by pre-treatment with APS. Discussion: The addition of APS to the culture can obviously regulate the expression of molecules related to the adhesion, growth, and immune response of ECs, as well as the production of cytokines. Therefore, it may have the potential to be an effective component in vaccines against CSFV.

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“…CSFV infection induced a functional decrease in the pro-coagulant activity of the ECs, which were highly susceptible to CSFV infection (Campos et al, 2004). The inhibitory effects of APS on porcine viruses infection have verified (Xue et al, 2017) and APS has been observed to regulate the levels of immunomodulating compounds in many different cell types (Wu et al, 2017; Huang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFVin vitro

Zhuge

Dong

et al. 2017

PeerJ

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BackgroundAstragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown.MethodsThe virus was titrated using an indirect immune biotin enzyme standard method to confirm that porcine ECs were susceptible to CSFV infection and to determine the TCID50 of CSFV (C-strain). Porcine ECs were cultured with CSFV in the presence of APS. Relative quantitative PCR was used to assess the mRNA expression of factors that influence EC adhesion and immunity.ResultsThe expression of adhesion factors mRNA increased following stimulation with CSFV; this effect was inhibited by pre-exposing the cells to APS. In addition, the expression of growth factors and some immune factors increased after infection with CSFV; this increase in tissue factor (TF), transforming growth factor (TGF-β), and interleukin-8 (IL-8) could be inhibited by the addition of APS. The immune response mediated by Toll-like receptor 4 (TLR4) in ECs may be unregulated by CSFV as it was also inhibited by pre-treatment with APS.DiscussionThe addition of APS to the culture can obviously regulate the expression of molecules related to the adhesion, growth, and immune response of ECs, as well as the production of cytokines. Therefore, it may have the potential to be an effective component in vaccines against CSFV.

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In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line

Cited by 10 publications

References 25 publications

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

Peer Review #1 of "Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFV in vitro (v0.2)"

Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFVin vitro

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