In vitro Effect of Benzimidazole Drugs on the Enzymes of Lipid Metabolism in Trichuris globulosa

Sarwal, S; Sanyal, Sankar Nath; Khera, S.

doi:10.2307/3283070

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…. Inhibition in the activity of G-6 Pase in Trichuris globulosa was shown with ethanolic neem leaf extract [11] . .…”

Section: Discussionmentioning

confidence: 96%

Evaluation of anthelmintic activity of ethanolic and aqueous leaf extracts of Azadirachta indica on phosphatases in Gastrothylax indicus

Aggarwal¹,

Bagai²

2014

IOSRJPBS

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Because of increasing anthelmintic resistance and the impact of conventional anthelmintics on the environment, it is important to look for alternative strategies against helminth parasite in sheep. The present study was performed to evaluate in vitro anthelmintic activity of plant Azadirachta indica on rumen amphistome of sheep.Anthelmintic activity of ethanolic and aqueous extracts of A. indica leaves in comparison with albendazole (positive control) and PBS (negative control) was evaluated through in vitro studies using worm motility inhibition and tegumental enzyme assays. Gastrothylax indicus was exposed in vitro to ethanolic and aqueous extracts(6.25 to 50 mg) of neem leaf to evaluate its anthelmintic efficacy. Mortality was observed at 2.14± 0.28h and 1.71± 0.21h for ethanolic and aqueous extracts respectively for the parasite at the highest test concentration of the plant extract. Anthelmintic albendazole was observed to cause mortality instantly (0.08±0.01h) at 80 μg/ml. Tegumental enzymes of the parasite ALP, ACP, ATPase and G-6-pase were found to be suppressed both by ethanolic and aqueous extracts. Enzyme kinetic studies showed inhibition to be noncompetitive in case of ALP with both the extracts whereas for ACP it was found to be non-competitive with ethanolic and competitive with aqueous extract. Albendazole showed competitive inhibition in case of ALP and non-competitive with ACP.

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“…. Inhibition in the activity of G-6 Pase in Trichuris globulosa was shown with ethanolic neem leaf extract [11] . .…”

Section: Discussionmentioning

confidence: 96%

Evaluation of anthelmintic activity of ethanolic and aqueous leaf extracts of Azadirachta indica on phosphatases in Gastrothylax indicus

Aggarwal¹,

Bagai²

2014

IOSRJPBS

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“…Recent studies have shown that mebendazole acts as a competitive inhibitor of cMDH enzyme in certain parasites (Sarwal et al 1989). Sanchez et al (1987) demonstrated that cMDH from other helminthes are also inhibited by several benzimidazole derivatives, including mebendazole.…”

Section: Discussionmentioning

confidence: 98%

Enzymatic and physico-chemical characteristics of recombinant cMDH and mMDH of Clonorchis sinensis

Zheng¹,

Huang²,

et al. 2006

Parasitol Res

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The cytosol and mitochondrial malate dehydrogenases (MDHs, EC 1.1.1.37) of Clonorchis sinensis were expressed in Escherichia coli as a fusion protein with a 6xHis and GST tag, respectively. The cytosol MDH of Clonorchis sinensis (Cs-cMDH) has higher resistibility to acid than mitochondrial MDH (Cs-mMDH). The Cs-cMDH also has higher heat resistibility and thermal stability than Cs-mMDH. Although there is only 22.8% identity between the amino acid sequences of Cs-cMDH and Cs-mMDH, they share several conserved residues. There are some differences between the circular dichroism spectra of Cs-cMDH and Cs-mMDH, but they have approximate percentages of helix. 4,4'-Bisdimethylamino diphenylcarbinol can decrease the Cs-mMDH activity but not the Cs-cMDH activity. Paraziquantel, metronidazole and albendazole did not inhibit the enzymes' activity, but adenosine 5'-monophosphate showed competitive inhibition to enzyme, with the Ki for Cs-cMDH and Cs-mMDH being 2.81 and 0.49 mM, respectively.

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“…Non competitive inhibition was reported for glycogen phosphorylase and ATPase whereas it was found to be mixed type for G-6-pase [33].…”

Section: Discussionmentioning

confidence: 99%

“…Other plant extracts such that of Alpinia nigra shoot-extract showed similar effect on ALP, ACP and ATPase activities of Fasciolopsis buski [32] . Inhibition in the activity of G-6 Pase in Trichuris globulosa was shown with ethanolic neem leaf extract [33] .…”

Section: Discussionmentioning

confidence: 99%

Impact of anthelmintic efficacy of Calotropis procera on tegumental enzymes of the trematode, Gastrothylax indicus

Aggarwal¹,

Bagai²

2014

iosrphr

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Abstract:The trematode parasite, Gastrothylax indicus was exposed to ethanolic and aqueous flower extract of Calotropis procera to evaluate the anthelmintic efficacy of the plant. The parasites were incubated in 6. 25, 12.5, 25, 50 was found to be suppressed by 43.890, 30.287,18.970 and 22.842% by ethanolic extract and 62.710, 19.780, 57.554 and 10.035% by aqueous extract whereas albendazole inhibited 41.617, 25.650, 64.797 and 26.611% respectively. Enzyme kinetic studies showed inhibition to be non-competitive in case of ACP with both the extracts and albendazole whereas for ALP it was found to be non-competitive with ethanolic and mixed type with aqueous extract. Albendazole showed competitive inhibition in case of ALP.

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In vitro Effect of Benzimidazole Drugs on the Enzymes of Lipid Metabolism in Trichuris globulosa

Cited by 9 publications

References 12 publications

Evaluation of anthelmintic activity of ethanolic and aqueous leaf extracts of Azadirachta indica on phosphatases in Gastrothylax indicus

Evaluation of anthelmintic activity of ethanolic and aqueous leaf extracts of Azadirachta indica on phosphatases in Gastrothylax indicus

Enzymatic and physico-chemical characteristics of recombinant cMDH and mMDH of Clonorchis sinensis

Impact of anthelmintic efficacy of Calotropis procera on tegumental enzymes of the trematode, Gastrothylax indicus

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