In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt

Tan, Xin; Riau, Andri K.; Shi, Z. L.; Tan, Anna; Neoh, K. G.; Khor, K.A.; Beuerman, Roger W.; Tan, Donald

doi:10.1136/bjophthalmol-2012-301633

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…A potential anti-inflammatory biomaterial would be useful as a skirt material in patients with chronic inflammatory states (e.g., Stevens-Johnson syndrome). 49 In our study conducted with a rabbit corneal stromal implantation model, slit-lamp biomicroscopy did not show any corneal haze, or any sign of neovascularization or inflammation. The AS-OCT imaging showed that an intact Gfilm did not lead to any changes in corneal stroma in the short term, and confocal imaging showed that the epithelium was intact with normal cell-cell interactions.…”

Section: Discussionmentioning

confidence: 98%

Application of Graphene as Candidate Biomaterial for Synthetic Keratoprosthesis Skirt

Tan

Thompson

Konstantopoulos

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Synthetic keratoprostheses are required for visual rehabilitation in patients with endstage corneal blindness. This study aimed to assess the biocompatibility of graphene material and its potential as a novel synthetic keratoprosthesis skirt material for corneal tissue engineering. METHODS.Human corneal stromal fibroblasts were cultured on material surfaces including pristine graphene film, graphene foam, pristine titanium (Ti) discs, and tissue culture plastic surface (TCPS). Cell attachment was assayed by immunostaining of paxillin and vinculin. Cell viability and proliferation were assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Click iT 5-ethynyl-2 0 -deoxyuridine (EdU) assays. The growth of fibroblasts on three-dimensional graphene foam was examined by scanning electron microscopy, and cytokine release was analyzed by enzyme-linked immunosorbent assay. Graphene films were implanted into rabbit corneal stromal pockets and examined by slit-lamp biomicroscopy, anterior segment optical coherence tomography, in vivo confocal microscopy, and histology.RESULTS. Pristine graphene demonstrated good biocompatibility with human stromal fibroblasts in terms of cell adhesion, viability, and proliferation. Cells on graphene films showed higher number than on TCPS control. Cells grown on graphene had 10% more proliferation than on Ti. The expression levels of IL-6 and IL-8 were reduced when cells were seeded on graphene foam as compared to Ti and graphene film. Implantation of graphene film into rabbit stroma (n ¼ 6) did not show any signs of infection, neovascularization, or inflammation.CONCLUSIONS. Graphene displayed excellent short-term biocompatibility with corneal cells and tissue. This demonstrates that graphene can be developed as a tissue engineering material for use in cornea.

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Section: Discussionmentioning

confidence: 98%

Application of Graphene as Candidate Biomaterial for Synthetic Keratoprosthesis Skirt

Tan

Thompson

Konstantopoulos

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…By virtue of its close resemblance to natural bone mineral and tooth enamel, hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , HA) has become a key bioceramic which has attracted significant interest in both materials research and clinical applications. Various in vitro and in vivo investigations such as cell culture and animal testing have been utilized to assess the biocompatibility and bioactivity of HA-based medical devices [1,2]. Such tests could be facilitated by using transparent HA-based structures as they enable direct visual observation of biomaterial -cell/tissue interfaces [3].…”

Section: Introductionmentioning

confidence: 99%

Transparent Hydroxyapatite Obtained through Spark Plasma Sintering: Optical and Mechanical Properties

Khor

2014

KEM

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Transparent bioceramics have great potential for applications in the biomedical field as they facilitate direct observation of the interactions at biomaterial – cell / tissue interfaces. Thus far, sintering of transparent hydroxyapatite (HA) usually involves application of extraordinarily high pressure and / or long duration. This study attempts to fabricate transparent HA by a direct and fast Spark Plasma Sintering (SPS) process using three different types of raw powder: micro-spheres (MS), nanorods (NR) and nanospheres (NS). The optical and mechanical properties of the sintered pellets were examined and compared. The highest total forward transmittance (TFT) showed by sintered MS pellet (~2 mm thick) was 85% in the visible spectrum, whereas sintered NR and NS pellets were either translucent or opaque. Although lowest degree of transparency was observed for NS pellets, they demonstrated highest Young’s modulus (E), hardness (H) and fracture toughness (KIC). The eminent KIC of NS pellets benefited mainly from its self-toughened microstructure.

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“…Titanium also provides excellent tissue tolerance in biological implants and has many special properties, including high resistance to corrosion, lightness and strength. 9,10 These characteristics allow the titanium back plate to be thinner (titanium back plate has an edge thickness of 0.25 mm compared to 0.8 mm central and 0.6 mm peripheral thickness in the PMMA back plate). Titanium is nonmagnetic, therefore patients can be subjected to magnetic resonance imaging.…”

Section: Design Development: Evolution Of the Boston Kpro Typementioning

confidence: 99%

<p>Boston Type 1 Keratoprosthesis: Updated Perspectives</p>

Nonpassopon¹,

Niparugs²,

Cortina³

2020

OPTH

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The use of Boston type 1 keratoprosthesis (BKPro) has significantly increased worldwide. It is no longer considered a procedure of last resort but a reasonable option for patients with otherwise poor prognosis for a traditional penetrating keratoplasty. BKPro was approved by the Food and Drug Administration in 1992 for bilateral severe corneal blindness due to multiple corneal transplant failure. Over the years, indications have extended beyond recurrent immunologic rejection to include other conditions such as chemical injury and other causes of bilateral limbal stem cell deficiency, extensive corneal neovascularization, neurotrophic corneas and hypotony, among others. Numerous advances in the design of the BKPro, improvement of preoperative, intraoperative and postoperative management have resulted in favorable outcomes and a reduction in postoperative complications. Accordingly, many studies have shown that implantation of this device is highly effective in restoring vision with very good short-term outcomes. However, due to the lifetime risk of sight-threatening complications after BKPro implantation, a longer follow-up period should provide outcomes that are more realistic. In this review, the authors examined only the results of publications with an average of at least 2 years of follow-up. The overall intermediate to long-term visual outcomes and retention rate in BKPro seem to be favorable. However, autoimmune diseases and cicatrizing conditions continue to show a higher incidence of postoperative complications that require further management.

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In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt

Cited by 11 publications

References 26 publications

Application of Graphene as Candidate Biomaterial for Synthetic Keratoprosthesis Skirt

Application of Graphene as Candidate Biomaterial for Synthetic Keratoprosthesis Skirt

Transparent Hydroxyapatite Obtained through Spark Plasma Sintering: Optical and Mechanical Properties

<p>Boston Type 1 Keratoprosthesis: Updated Perspectives</p>

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