In vitro Dissolution of Calcium Oxalate Stones with Ethylenediaminetetraacetic Acid and Snake Venom Thrombin-Like Enzyme

Zhou, Xiangjun; Zhang, Jie; Ci, Zhang; Xu, Changgeng

doi:10.1159/000353096

Cited by 7 publications

(9 citation statements)

References 18 publications

(18 reference statements)

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“…Some findings suggested that chemical treatments may be useful for improving the efficacy of stone treatment via dissolving larger or harder stones. Zhou et al, 27 reported that buffered EDTA solvents might be feasible chemical treatment modalities for improving the efficacy of calcium oxalate stone dissolution.…”

Section: Results: Phytochemical Screening and Quantitative Estimation Of Phytoconstituentsmentioning

confidence: 99%

“…These phytoconstituents are of utmost significance for inhibiting urinary stone formation. Saponins possess antilithic properties and are known to disintegrate mucoproteins that are crucial components of the stone matrix 27 . Rutin, quercetin, hyperoside, and diosmin are known as flavonoids with high antioxidant and antilithiatic activities 28 .…”

Section: Results: Phytochemical Screening and Quantitative Estimation Of Phytoconstituentsmentioning

confidence: 99%

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Untitled

2020

IJPSR

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Seeds of Macrotyloma uniflorum Linn. (Fabaceae) are widely used for treatment in kidney stones in India. In This study evaluates the invitro effect of methanolic extract of Macrotyloma uniflorum (MEMU) on calcium oxalate crystallization. A methanolic extract of M. uniflorum seeds at different concentrations (250-1500 μg/ml) was subjected to in-vitro crystallization activity. The obtained results were compared with a Cystone (standard herbal product) available in the market. The nucleation and crystal growth assay was performed with different concentrations of sodium oxalate (2-10 mmol/ml for nucleation, 2-3.5 mmol/ml for crystal growth) and evaluated the effect of MEMU and cystone at different doses (250-1500 μg/ml) on the different concentration level of oxalate. Crystal aggregation and crystal dissolution assay were performed and compared with cystone effect. The MEMU was significantly more effective than cystone at inhibiting the nucleation rate and crystal growth at a different concentration level of oxalate. Inhibition of calcium oxalate crystal aggregation and dissolution was observed in a dose-dependent manner. The results showed that MEMU has excellent in-vitro calcium oxalate crystallization inhibition activity in the different concentration level of oxalate and also have crystal dissolution activity; therefore, it might be useful in the prevention of renal stones.INTRODUCTION: Kidney stones are the solid crystalline masses that occur anywhere in the urinary system, and it is often correlated with habits such as high purine intakes, obesity, hypertension, metabolic syndrome, and diabetes 1 . A large number of people, nearly 4-15% of the human population, suffer from the renal stone problem all over the global 2 .

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Section: Results: Phytochemical Screening and Quantitative Estimation Of Phytoconstituentsmentioning

confidence: 99%

Section: Results: Phytochemical Screening and Quantitative Estimation Of Phytoconstituentsmentioning

confidence: 99%

Untitled

2020

IJPSR

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“…Some findings suggested that chemical treatments may be a useful for improving the efficacy of stone treatment via dissolving larger or harder stones. Zhou et al 30 reported that buffered EDTA solvents may be feasible chemical treatment modalities for improving the efficacy of CaOx stone dissolution. In present study, nBFMU was found active in dissolving the CaOX stones in concentration depended manner, which indicate that nBFMU contain some of the components that constitutes to increase the CaOX stone dissolution.…”

Section: Discussionmentioning

confidence: 99%

Anti-urolithiatic activities of Macrotyloma uniflorum Mediated through Multiple Pathway

Patel¹,

Acharya²

2020

IJPER

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Background: Macrotyloma uniflorum Linn. (Fabaceae) seeds are widely used for their diuretic and urolithiatic effects in India. The present study investigated the effect of n-butanol fraction of M. uniflorum (nBFMU) on kidney stone using in vitro and in vivo method. Materials and Methods: Nucleation, crystal growth, crystal aggregation and crystal dissolution assays were performed for nBFMU. Two doses of nBFMU (400 and 800 mg/kg) were studied for their diuretic activity and sodium oxalate (NaOx) (70 mg/kg, i.p) induced urolithasis in male Wistar albino rats. Determination of body and kidney weight, measurement of various biochemical parameters in biological samples and examination of histology of kidney at the end of experiment were also done. Results: nBFMU exhibited a concentration dependent inhibitory activity on nucleation and aggregation along with decreased number of crystals of calcium oxalate (CaOX) produced in metastable solutions of CaOX in the in vitro experiments. Co-administration of nBFMU with NaOX has significantly (p<0.001) increased the urine volume and the level of calculus inhibitors like magnesium, citrate and decreased the level of calculus promoters like oxalate, calcium, urea and uric acid. nBFMU supplement also prevented the pathological changes in kidney and increased the glomerulus activity of the kidney. Conclusion: These results indicate that nBFMU showed significant activity in urolithiasis which might be due to its diuretic, CaOX crystal formation inhibitory effects and its ability to increase the levels of inhibitors and decrease the level of promoters of urolithiasis.

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“…Thousands of infected bladder stones were removed; stones weighed 612 g and the number of stones was about 1,500. Stone analysis showed that 70% was magnesium ammonium phosphate and 20% ammonium urate, rather than calcium oxalate, which is generally the most predominant (70-80%) type of urinary tract stone [1]. At 2 months of follow-up, the serum creatinine was 81 μmol/l and the hydronephrosis and hydroureter were significantly improved, while ultrasonic images showed that new stones could be observed in the bladder.…”

Section: Figmentioning

confidence: 99%