In-vitro dissolution and structural and electrokinetic characteristics of titanium-oxynitride coatings formed via reactive magnetron sputtering

Пичугин, В. Ф.; Pustovalova, A. A.; Konishchev, M. E.; Хлусов, И. А.; Иванова, Н. М.; Sun, Zheng; Gutor, Sergey

doi:10.1134/s1027451016020166

Cited by 23 publications

(18 citation statements)

References 32 publications

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“…The electrokinetic (ζ) potential was determined at Far East Federal University (Vladivostok, Russia) using a Z-potentiometer for solid surface analysis (SurPASS 3, Anton Paar GmbH, Graz, Austria) in 0.05 M KCl aqueous solution as described in [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

et al. 2020

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Calcium phosphate (CaP) materials are among the best bone graft substitutes, but their use in the repair of damaged bone in tumor patients is still unclear. The human Jurkat T lymphoblast leukemia-derived cell line (Jurkat T cells) was exposed in vitro to a titanium (Ti) substrate (10 × 10 × 1 mm3) with a bilateral rough (average roughness index (Ra) = 2–5 μm) CaP coating applied via the microarc oxidation (MAO) technique, and the morphofunctional response of the cells was studied. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscope (EDX) analyses showed voltage-dependent (150–300 V) growth of structural (Ra index, mass, and thickness) and morphological surface and volume elements, a low Ca/PaT ratio (0.3–0.6), and the appearance of crystalline phases of CaHPO4 (monetite) and β-Ca2P2O7 (calcium pyrophosphate). Cell and molecular reactions in 2-day and 14-day cultures differed strongly and correlated with the Ra values. There was significant upregulation of hTERT expression (1.7-fold), IL-17 secretion, the presentation of the activation antigens CD25 (by 2.7%) and CD95 (by 5.15%) on CD4+ cells, and 1.5–2-fold increased cell apoptosis and necrosis after two days of culture. Hyperactivation-dependent death of CD4+ cells triggered by the surface roughness of the CaP coating was proposed. Conversely, a 3.2-fold downregulation in hTERT expression increased the percentages of CD4+ cells and their CD95+ subset (by 15.5% and 22.9%, respectively) and inhibited the secretion of 17 of 27 test cytokines/chemokines without a reduction in Jurkat T cell survival after 14 days of coculture. Thereafter, cell hypoergy and the selection of an hTERT-independent viable CD4+ subset of tumor cells were proposed. The possible role of negative zeta potentials and Ca2+ as effectors of CaP roughness was discussed. The continuous (2–14 days) 1.5–6-fold reductions in the secretion of vascular endothelial growth factor (VEGF) by tumor cells correlated with the Ra values of microarc CaP-coated Ti substrates seems to limit surgical stress-induced metastasis of lymphoid malignancies.

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Section: Methodsmentioning

confidence: 99%

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

et al. 2020

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“…The TiO x N y -surfaces fabricated by a microwave-assisted process show a higher photocatalytic activity of nitrogen-containing films providing better suitability for medical applications [73]. The investigative results of titanium-oxynitride (TiO x N y ) coatings deposited on L316 stainless steel by reactive magnetron sputtering are presented in [74]. The described coatings are highly biocompatible, possess a negatively charged surface and negative zeta potential, prevents in vitro adhesion of salts on the surface, and alters the surface wettability.…”

Section: Titanium Oxide and Titanium Oxynitride Coatingsmentioning

confidence: 99%

“…(ii) Titanium oxide inhibits the electron transfer from fibrinogen to the surface, reducing platelet aggregation and fibrinogen coagulation; and (iii) nitric oxide (NO) released from the coating performs the necessary biological functions, promotes endothelialization, activating the growth of endothelial cells. It is important to study the structural features and properties of nitrogen-containing films of titanium dioxide formed by ion-plasma methods and to establish the relationship between the features of the microstructure of the films and the conditions of their deposition [74][75][76][77][78]. The problem of applying a uniform, stable coating that retains high physicochemical and adhesive properties after opening the stent remains open [60].…”

Section: Titanium Oxide and Titanium Oxynitride Coatingsmentioning

confidence: 99%

Recent Advances in Manufacturing Innovative Stents

et al. 2020

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Cardiovascular diseases are the most distributed cause of death worldwide. Stenting of arteries as a percutaneous transluminal angioplasty procedure became a promising minimally invasive therapy based on re-opening narrowed arteries by stent insertion. In order to improve and optimize this method, many research groups are focusing on designing new or improving existent stents. Since the beginning of the stent development in 1986, starting with bare-metal stents (BMS), these devices have been continuously enhanced by applying new materials, developing stent coatings based on inorganic and organic compounds including drugs, nanoparticles or biological components such as genes and cells, as well as adapting stent designs with different fabrication technologies. Drug eluting stents (DES) have been developed to overcome the main shortcomings of BMS or coated stents. Coatings are mainly applied to control biocompatibility, degradation rate, protein adsorption, and allow adequate endothelialization in order to ensure better clinical outcome of BMS, reducing restenosis and thrombosis. As coating materials (i) organic polymers: polyurethanes, poly(ε-caprolactone), styrene-b-isobutylene-b-styrene, polyhydroxybutyrates, poly(lactide-co-glycolide), and phosphoryl choline; (ii) biological components: vascular endothelial growth factor (VEGF) and anti-CD34 antibody and (iii) inorganic coatings: noble metals, wide class of oxides, nitrides, silicide and carbide, hydroxyapatite, diamond-like carbon, and others are used. DES were developed to reduce the tissue hyperplasia and in-stent restenosis utilizing antiproliferative substances like paclitaxel, limus (siro-, zotaro-, evero-, bio-, amphi-, tacro-limus), ABT-578, tyrphostin AGL-2043, genes, etc. The innovative solutions aim at overcoming the main limitations of the stent technology, such as in-stent restenosis and stent thrombosis, while maintaining the prime requirements on biocompatibility, biodegradability, and mechanical behavior. This paper provides an overview of the existing stent types, their functionality, materials, and manufacturing conditions demonstrating the still huge potential for the development of promising stent solutions.

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“…The ion deposition on artificial surfaces with salt precipitation is well known. [24] On a-C:H:SiO x -deposited substrates, this process is slower, aside from for the ethanol-and miramistintreated substrates. On the other hand, Ca 2+ release into the solution from a-C:H:SiO x -deposited substrates after sterilization by Chemicals 1-3.…”

Section: Analysis Of Extracts In Liquid Nutrient Medium Dmem/f12mentioning

confidence: 99%

Stability of a‐C:H:SiO_x coating on polypropylene to chemical sterilization

Grenadyorov

Solovyev

Малащенко

et al. 2020

J of Applied Polymer Sci

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Polypropylene (PP) is traditionally used in the production of medical devices, such as catheters, dialyzers, syringes due to its good chemical and thermal resistance, biocompatibility, and low cost. Sterilization can however damage these devices, when the polymer additive releases into physiological fluids, thereby harming the patient health. This article proposes to deposit biocompatible a‐C:H:SiOx coatings onto PP surfaces and studies the coating stability after chemical sterilization. Five different chemical sterilizers, namely, hydrogen peroxide, ethanol, miramistin, formaldehyde, and ethylene oxide are used to compare their effect on the coating stability on the PP substrate. Untreated and sterilized samples are incubated in a liquid synthetic nutrient to obtain extracts that are used for sterility testing and determining the degree of interaction of samples with the nutrient medium. The changes in the coating functional groups and surface morphology are examined by using the Fourier transform infrared spectroscopy and the scanning electron microscopy, respectively. It is shown that the a‐C:H:SiOx coating has a protective effect on the PP substrates subjected to chemical sterilization. The experiment results clearly showed that the ethanol sterilization causes the most serious changes in the structure of PP, and also leads to the barrier coating failure. On the other hand, ethylene oxide sterilization has a minimal effect on the structure of both uncoated and coated PP substrates.

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In-vitro dissolution and structural and electrokinetic characteristics of titanium-oxynitride coatings formed via reactive magnetron sputtering

Cited by 23 publications

References 32 publications

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

Recent Advances in Manufacturing Innovative Stents

Stability of a‐C:H:SiO_x coating on polypropylene to chemical sterilization

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In-vitro dissolution and structural and electrokinetic characteristics of titanium-oxynitride coatings formed via reactive magnetron sputtering

Cited by 23 publications

References 32 publications

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

Recent Advances in Manufacturing Innovative Stents

Stability of a‐C:H:SiOx coating on polypropylene to chemical sterilization

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Product

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Stability of a‐C:H:SiO_x coating on polypropylene to chemical sterilization