In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells

Hong, Seung Hyun; Gang, Eun Ji; Jeong, Ju Ah; Ahn, Chiyoung; Hwang, Soo Han; Yang, In; Park, Hwon Kyum; Han, Hoon; Kim, Hoeon

doi:10.1016/j.bbrc.2005.03.086

Cited by 234 publications

(167 citation statements)

References 48 publications

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“…MSCs, derived from mesoderm and neural crest, are adult progenitor cells with a multidirectional differentiation potential (Slukvin and Vodyanik 2011;Vodyanik et al 2010;Takashima et al 2007). Studies have demonstrated that MSCs can differentiate into hepatocytes because of their mesenchymal differentiation capabilities (Hong et al 2005;Lee et al 2004;Snykers et al 2009). Moreover, MSCs have immunosuppressive properties (Bartholomew et al 2002;Di Nicola et al 2002;Glennie et al 2005;Krampera et al 2003;Le Blanc and Ringden 2005).…”

Section: Introductionmentioning

confidence: 99%

Icariin-treated human umbilical cord mesenchymal stem cells decrease chronic liver injury in mice

et al. 2016

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Human umbilical cord mesenchymal stem cells (hUMSCs) have been shown to have multiple differentiation potentials. However, a key problem is that only a small number of hUMSCs can migrate to damaged tissue after transplantation. According to ''The Theory of Kidney Essence'' in Traditional Chinese Medicine, some traditional Chinese medicines used for tonifying the kidneys can be applied in promoting the differentiation and migration of stem cells in vivo. Our previous study demonstrated that icariin (ICA) could up-regulate the pluripotent genes of hUMSCs in vitro and induce cell migration in mice in an acute kidney injury model in vivo. The aim of this study was to investigate the effects of ICAinduced hUMSCs in chronic liver injury (CLI) caused by carbon tetrachloride (CCl 4 ). CLI was induced by intraperitoneal injection of CCl 4 . ICA-treated hUMSCs were transplanted via intra-venous injection. The animals were followed for survival, biochemistry analysis and pathology. The results show that ICAtreated hUMSCs accelerate the recovery of liver function in mice with CLI. In addition, ICA-treated hUMSCs increase the anti-oxidant activities in liver and prevent the progression to hepatic fibrosis. Moreover, ICA induces the migration of hUMSCs to the injured liver tissue. In conclusion, these data demonstrate that ICA-treated hUMSCs exhibit recovery and protective properties in the mice model of CCl 4 -induced CLI.

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Section: Introductionmentioning

confidence: 99%

Icariin-treated human umbilical cord mesenchymal stem cells decrease chronic liver injury in mice

et al. 2016

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“…As well as HSCs, UCB contains stem cells with the properties of MSCs (Kern et al, 2006;Wagner et al, 2005). It has been reported that, after stimulation with HGF, DEX, and OsM, UCB-MSCs differentiate into hepatocyte-like cells (Hong et al, 2005;. Functional hepatocytes, together with BM-MSCs, were generated by another group .…”

Section: Umbilical Cord Blood Mesenchymal Stem Cells (Ucbmscs)mentioning

confidence: 99%

Stem cell plasticity: Learning from hepatogenic differentiation strategies

Banas¹,

Yamamoto

Teratani³

et al. 2007

Developmental Dynamics

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Many studies on stem cell plasticity are challenging the concept that stem cells contain an intrinsically predefined, unidirectional differentiation program. This means that the developmental fate of a stem cell is dependent on the general potential of the cell (pre-determined stem cell fate) as well as on microenvironmental cues, such as stimuli from growth factors (stem cell niche). Here, we reviewed reports that examined the hepatocyte differentiation ability of stem cells from two different sources: embryonic stem cells and adult stem cells. All of those stem cells revealed the ability to give rise to hepatocyte-like cells using different induction strategies. However, it is still not clear which of those stem cells would be the best source for hepatocyte replacement or which would be the best protocol. We herein present the current knowledge regarding available protocols and factors used in order to obtain functional hepatocytes from stem cells. Developmental Dynamics 236:3228 -3241, 2007.

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“…38 This has been demonstrated by coculture of MSCs, e.g., with foetal liver cells although the adopted hepatocyte phenotype displays limited hepatocytes function, such as secretion of urea and cytochrome P450 activity. 39 Hepatogenic differentiation of MSCs from other sources, such as adipose tissue 40 or umbilical cord blood, 41 was achieved in vitro, too, again without sufficient proof of their phenotype in vivo. Nevertheless, when human BM stem cell fractions were directly xenografted into rat liver damaged with allyl alcohol, only the fraction of MSCs developed into hepatocyte-like cells, positive for mRNA albumin expression.…”

Section: Differentiation Of Bm-derived Mscs Towards Liver Progenitor mentioning

confidence: 99%

Adult stem cells in progression and therapy of hepatocellular carcinoma

Herr

Groth

Schemmer

et al. 2007

Intl Journal of Cancer

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Hepatocellular carcinoma is one of the most aggressive solid tumours associated with poor prognosis. Despite its significance, there is only an elemental understanding of the mechanisms that drive disease pathogenesis, and there are just limited therapy options. The medical community is currently experiencing a wave of enthusiasm for clinical trials, in which adult stem/progenitor cells are used for liver regeneration. This is based on promising results in animal models and encouraging reports from some initial clinical studies. On the other hand, several essential precautions are not being fully addressed. Stem cells may contribute to fibrosis or give rise to hepatic cancer stem cells as a source of hepatocellular carcinoma. This review outlines the current state of knowledge in progression of liver disease and highlights the function of adult stem cells in disease and therapy.

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In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells

Cited by 234 publications

References 48 publications

Icariin-treated human umbilical cord mesenchymal stem cells decrease chronic liver injury in mice

Icariin-treated human umbilical cord mesenchymal stem cells decrease chronic liver injury in mice

Stem cell plasticity: Learning from hepatogenic differentiation strategies

Adult stem cells in progression and therapy of hepatocellular carcinoma

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