In Vitro Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Hair Cells
            Using Growth Factors

Mahmoudian-Sani, Mohammad-Reza; Hashemzadeh‐Chaleshtori, Morteza; Jami, Mohammad‐Saeid; Saidijam, Massoud

doi:10.5935/0946-5448.20170030

Cited by 14 publications

(6 citation statements)

References 27 publications

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“…The ability to use bone marrow mesenchymal stem cells (BMSCs) in the inner ear has already been examined [Mahmoudian-Sani et al, 2017a;Naito et al, 2004]. Transplanted BMSCs are able to migrate to different parts in the cochlea [Sharif et al, 2007].…”

Section: Bone Marrow Mesenchymal Stem Cells In Treating Hearing Lossmentioning

confidence: 99%

The Effect of the MicroRNA-183 Family on Hair Cell-Specific Markers of Human Bone Marrow-Derived Mesenchymal Stem Cells

et al. 2018

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Hearing loss is considered the most common sensory disorder across the world. Nowadays, a cochlear implant can be an effective treatment for patients. Moreover, it is often believed that sensorineural hearing loss in humans is caused by loss or disruption of the function of hair cells in the cochlea. In this respect, mesenchymal cells can be a good candidate for cell-based therapeutic approaches. To this end, the potential of human bone marrow-derived mesenchymal stem cells to differentiate into hair cells with the help of transfection of microRNA in vitro was investigated. MicroRNA mimics (miRNA-96, 182, and 183) were transfected to human bone marrow-derived mesenchymal stem cells using Lipofectamine as a common transfection reagent following the manufacturer’s instructions at 50 nM for microRNA mimics and 50 nM for the scramble. The changes in cell morphology were also observed under an inverted microscope. Then, the relative expression levels of SOX2, POU4F3, MYO7A, and calretinin were assayed using real-time polymerase chain reaction according to the ΔΔC_t method. The ATOH1 level was similarly measured via real-time polymerase chain reaction and Western blotting. The results showed that increased expression of miRNA-182, but neither miRNA-96 nor miRNA-183, could lead to higher expression levels in some hair cell markers. The morphology of the cells also did not change in this respect, but the evaluation of gene expression at the levels of mRNA could promote the expression of the ATOH1, SOX2, and POU4F3 markers. Furthermore, miRNA-182 could enhance the expression of ATOH1 at the protein level. According to the results of this study, it was concluded that miRNA-182 could serve as a crucial function in hair cell differentiation by the upregulation of SOX2, POU4F3, and ATOH1 to promote a hair cell’s fate.

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Section: Bone Marrow Mesenchymal Stem Cells In Treating Hearing Lossmentioning

confidence: 99%

The Effect of the MicroRNA-183 Family on Hair Cell-Specific Markers of Human Bone Marrow-Derived Mesenchymal Stem Cells

et al. 2018

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“…In the cochlea, regeneration of auditory neurons and hair cells through the application of MSCs is not a simple process. It requires certain neurotrophic factors especially glial cell-derived neurotrophic factor, brain-derived neurotrophic factor and neurotrophin-3 (GDNF, BDNF, NT-3) and growth factors for the differentiation of MSCs into auditory hair-like and neuronal cells (22)(23)(24). Evidence showed that mesenchymal stem cells could be able to differentiate auditory hair-like cells that were confirmed by the expression of hair cell markers Atoh1, Sox2, Jagged2, p27kip, Brn3c, Myosin VIIA and, Espin which are essential for inner ear development and hearing function (20).…”

Section: Mesenchymal Stem Cells In Inner Hair Cell Regenerationmentioning

confidence: 99%

Therapeutic Application of Mesenchymal Stem Cells for Cochlear Regeneration

Maharajan

Cho

Jang

2021

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“…Western blotting A total of 25 µg of proteins was loaded on a 12% NuPAGE (Novex, CA) and transferred onto PVDF membranes (Novex, CA) as described (Mahmoudian-Sani et al 2017;Rafiee et al 2019). Membranes were blocked with 5% skim milk in Tris-buffered saline and 0.01% tween 20 (TBST buffer) for 30 min at room temperature and then incubated overnight at 4 °C with either rabbit polyclonal anti-Cyp1A1 (Abcam, CA) or mouse monoclonal anti-GAPDH (Abcam, CA), diluted in TBST buffer containing 1% skim milk.…”

Section: Pathway Analysismentioning

confidence: 99%

Diesel exhaust exposure alters the expression of networks implicated in neurodegeneration in zebrafish brains

et al. 2021

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Neurodegenerative diseases are a major cause of disability in the world, but their etiologies largely remain elusive. Genetic factors can only account for a minority of risk for most of these disorders, suggesting environmental factors play a significant role in the development of these diseases. Prolonged exposure to air pollution has recently been identified to increase the risk of Alzheimer’s and Parkinson’s diseases, but the molecular mechanisms by which it acts are not well understood. Zebrafish embryos exposed to diesel exhaust particle extract (DEPe) lead to dysfunctional autophagy and neuronal loss. Here, we exposed zebrafish embryos to DEPe and performed high throughput proteomic and transcriptomic expression analyses from their brains to identify pathogenic pathways induced by air pollution. DEPe treatment altered several biological processes and signaling pathways relevant to neurodegenerative processes, including xenobiotic metabolism, phagosome maturation, and amyloid processing. The biggest induction of gene expression in brains was in Cyp1A (over 30-fold). The relevance of this expression change was confirmed by blocking induction using CRISPR/Cas9, which resulted in a dramatic increase in sensitivity to DEPe toxicity, confirming that Cyp1A induction was a compensatory protective mechanism. These studies identified disrupted molecular pathways that may contribute to the pathogenesis of neurodegenerative disorders. Ultimately, determining the molecular basis of how air pollution increases the risk of neurodegeneration will help in the development of disease-modifying therapies. Graphical abstract

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In Vitro Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Hair Cells Using Growth Factors

Cited by 14 publications

References 27 publications

The Effect of the MicroRNA-183 Family on Hair Cell-Specific Markers of Human Bone Marrow-Derived Mesenchymal Stem Cells

The Effect of the MicroRNA-183 Family on Hair Cell-Specific Markers of Human Bone Marrow-Derived Mesenchymal Stem Cells

Therapeutic Application of Mesenchymal Stem Cells for Cochlear Regeneration

Diesel exhaust exposure alters the expression of networks implicated in neurodegeneration in zebrafish brains

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