In vitro differentiation of endothelial cells (EC) from AC133-positive progenitor cells

Gehling, Ursula M.; Ergün, Süleyman; Schuch, Gunter; Schafhausen, Philippe; Kilic, Nerbil; Schäfer, Beat W.; Hossfeld, Dieter K.; Fiedler, Walter

doi:10.1016/s0959-8049(99)80794-1

Cited by 304 publications

(403 citation statements)

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“…3 Thus far, although the identity of EPCs has not been clear yet, the ex vivo cultivated EPCs, which already commit to differentiation at some levels, have been also proved to contribute to tumor vessel formation. 4,5 Ex vivo cultivated EPCs derived from mononuclear cells of human peripheral blood or cord blood have been successfully shown to incorporate into neovasculature and contribute to functional recovery of ischemic tissue. 6,7 In this study, we examined whether EPCs could promote tumor angiogenesis and found that the tumor growth was enhanced by the administration of EPCs.…”

Section: Discussionmentioning

confidence: 99%

Interferon-β efficiently inhibited endothelial progenitor cell-induced tumor angiogenesis

et al. 2011

Gene Ther

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Neovascularization has a critical role in the growth and metastatic spread of tumors, and involves recruitment of circulating endothelial progenitor cells (EPCs) from bone marrow. In this study, we examined whether EPCs could promote tumor angiogenesis, and found that the tumor growth was enhanced by the administration of EPCs. To test the hypothesis that genetically modified bone marrow-derived EPCs can be effective carriers of therapeutic agents to tumor sites, we conducted human interferon-beta (HuIFN-b) gene transfection of EPCs with a virus vector in vitro. When HuIFN-b was applied in the ex vivo culture of EPCs, HuIFN-b-transduced EPCs achieved efficient killing of the total population of SPC-A1 cells, indicating a bystander effect was elicited by HuIFN-b-transduced EPCs in vitro. When SCP-A1 cancer cells were coimplanted along with ex vivo cultivated EPCs subcutaneous injection in nude mice, the tumor growth was increased. However, the anti-tumor effect of interferon-beta (IFN-b) offset the tumor-progressive character of EPCs and the tumor growth, and the vascular density of tumor tissues increased by coimplanted EPCs were decreased upon IFN-b treatment. In addition, overall expression levels of vascular endothelial growth factor in tumor tissues were decreased upon IFN-b treatment. Therefore, our results suggest that gene-transfected EPCs could be useful as a tumor-specific drug delivery system.

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Section: Discussionmentioning

confidence: 99%

Interferon-β efficiently inhibited endothelial progenitor cell-induced tumor angiogenesis

et al. 2011

Gene Ther

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“…It has been reported that surface biomarker CD133 [19,20] is not present in embryonic stem cells. We tried to confirm this observation using our newly developed assay.…”

Section: Human Embryonic Stem Cell Biomarker Detection From a Single mentioning

confidence: 99%

Polymeric micelle-based bioassay with femtomolar sensitivity

Mouffouk

Chishti

Jin

et al. 2008

Analytical Biochemistry

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Target specific polymeric micelles loaded with fluorescence dye molecules in their hydrophobic cores were made from block co-polymer of poly(carprolactones) 23 -b-poly(ethylene oxide) 45 (PCL23-b-PEO45). It was found that the micelles are stable against pH changes from 2 to 12 and temperature variation up to 65 °C. The dye molecules can be released to the solution upon exposing the micelles to organic solvents or ultrasound. A rapid and highly sensitive immunoassay based on the above micelles was developed, and the assay can detect specific target proteins in femtomolar range from complex biological samples such as serum mimics and cell lysate. For example, less than 0.15 unit/ml of ovarian cancer specific antigen 125 (CA125), equivalent to 7.5 × 10 -15 M, can be reliably detected in solution. We also demonstrated the assay can detect a cell surface biomarker (SAAE-4) from a single human embryonic stem (hES) cell.

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“…[9][10][11][12] EPCs are characterized by CD34, Flk-1, and CD133 antigen-positive cells, 13,14 and these cells are localized predominantly in the bone marrow. 15 In the peripheral circulation of adults, more mature, differentiated EPCs are found that are characterized by a decreased expression of CD133, are positive for VEGFR-2, and still express CD34.…”

Section: Introductionmentioning

confidence: 99%

Involvement of circulating endothelial progenitor cells and vasculogenic factors in the pathogenesis of diabetic retinopathy

Lee

Chae

Kim

2005

Eye

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In vitro differentiation of endothelial cells (EC) from AC133-positive progenitor cells

Cited by 304 publications

References 0 publications

Interferon-β efficiently inhibited endothelial progenitor cell-induced tumor angiogenesis

Interferon-β efficiently inhibited endothelial progenitor cell-induced tumor angiogenesis

Polymeric micelle-based bioassay with femtomolar sensitivity

Involvement of circulating endothelial progenitor cells and vasculogenic factors in the pathogenesis of diabetic retinopathy

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