In Vitro Diagnosis of Allergy Radioallergosorbent Test (Rast) Studies With Some Allergens Commonly Encountered in Australia

Summary Thirty‐five asthmatic patients had prick skin tests to the common allergens Candida aibicans, Aspergillus fumigatus, grass pollen, horse dander and Dermatophagoides pteronyssinus performed on a regular basis from Autumn 1973 to Autumn 1975. Specific IgE to the same allergens (except C. albicans) was determined at the time of skin testing for the first five seasons. It was found that the position on the volar aspect of the forearm on which the test was performed did not affect the reaction. There was a significant variation in the percentage of patients with positive skin tests to A. fumigatus, grass pollen and horse dander with the latter showing a significant decrease with time. There was evidence of variation in weal size for all but C. albicans, and for grass pollen, horse dander and D. pteronyssinus there were reductions in weal size with time. Significant differences were found for results of Log specific IgE for grass pollen and D. pteronyssinus over the study, but there was no trend. A good correlation between weal size and Log specific IgE for grass pollens and D. pteronyssinus was observed. For the four allergens, the coefficient of concordance between IgE levels within patients for the five seasons was highly significant.

Section: Discussionmentioning

confidence: 99%

Reproducibility of prick skin tests to five common allergens

Clarke¹,

Mitchell²,

Nunn

et al. 1982

“…Results are shown in Table 1. A positive value in the RAST was taken as a radioactive uptake of greater than 2-5 times the cord/normal serum values [14][15][16]. Strong positive reactions to each ofthe blowfly and screw-worm fly extracts were detected but tests to all other insect extracts were negative.…”

Section: Examinations For Ige Antibodiesmentioning

confidence: 99%

Inhalant allergy following occupational exposure to blowflies

Kaufman

Baldo

Tovey

et al. 1986

Self Cite

An allergic reaction, provoked by exposure to the blowfly Lucilia cuprina and shown to be IgE-mediated, occurred in a subject employed in an entomological research laboratory. The subject's serum, and sera from three other asthmatic patients with IgE antibodies to blowfly extracts, also reacted with extracts from the screw-worm fly {Chrysomya bezziana). Results suggested that antigens from the two species share immunological cross-reactivity. Cross-reactions also exist between the difl"erent developmental stages of both species. Allergic reactions to inhaled insect allergens may not be uncommon in the Australian community.

“…Sera Cl and Sa gave uptakes of'-• I-anti-lgE of 9-1% and 24'3%, respectively. Based on previous experience by ourselves [9,16], and others [17]. where a RAST value of greater than 2-5 times the cord/normal value is considered positive, the uptakes by Cl and Sa clearly demonstrate the presence of trimethoprim-reactive IgE antibodies.…”

Section: Examination For Sulphamethoxazole-reactive Ige Antibodiesmentioning

confidence: 92%

An immunoassay for the detection of IgE antibodies to trimethoprim in the sera of allergic patients

Harle

Baldo

Smal

et al. 1987

Self Cite

An immunoassay was developed to detect IgE antibodies lo the widely used antibacterial drug trimethoprim. Significant levels of trimclhoprim-reactive IgE antibodies were found in the sera of two patients who had experienced life-threatening allergic reactions following administration of a combination of trimethoprim and sulphamethoxazole. No IgE antibodies reactive with sulphamethoxazole were found in the sera of either patient. Inhibition experiments revealed that a high degree of crossreactivity occurs between the drug-reactive IgE antibodies and two structural analogues of trimethoprim. 6-hydroxy-and 6-chloroirimethoprim. These experiments also indicated that the combining sites ofthe trimethoprim-reactivc IgE antibodies in the two sera were probably complementary to different parts o{ the trimethoprim molecule. The assay should supplement skin testing in determining the offending drug in patients with suspected allergie sensitivity to trimethoprim-sulphamethoxazole complex.