In-vitro development of zygotes from superovulated prepubertal and mature gilts

Pinkert, Carl A.; Kooyman, David L.; Baumgartner, Adi; Keisler, D.H.

doi:10.1530/jrf.0.0870063

Cited by 57 publications

(26 citation statements)

References 15 publications

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“…In our experiment, the highest number and percentage of hatched embryos were obtained from gilts in the control group. Similarly, in the study of Pinkert et al [12], zygotes obtained from prepubertal gilts after induction of superovulation did not possess the same in vitro developmental potential as zygotes from pubertal gilts. Moreover, the authors observed that the development of embryos from prepubertal and mature gilts was similar to the morula but not the blastocyst stage.…”

Section: Discussionmentioning

confidence: 74%

Influence of Estrus Synchronization of Prepubertal Gilts on Embryo Quality

Ziȩcik

Biallowicz

Kaczmarek

et al. 2005

Journal of Reproduction and Development

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Abstract. Synchronization and superovulation are commonly used to obtain large numbers of embryos for experimental and practical purposes. This study compared the number, quality, and in vitro development of embryos recovered from gilts following single or double estrus synchronization and superovulation. Prepubertal gilts from the single synchronization group were injected with 1500 I.U. PMSG and 1000 I.U. hCG 72 h later. The double synchronized group of gilts was treated with 750 I.U. PMSG and 500 I.U. hCG 72 h later. After 17 days, 1500 I.U. PMSG followed by 1000 I.U. hCG was administered. Five days after insemination embryos were recovered and cultured for 6 days. Both single and double hormonal stimulation schedules resulted in recovery of elevated numbers of embryos (28.4 and 23.4 vs. 11.3; p<0.01and p≤0.05, respectively) with a higher percentage of embryos classified as degenerated (39.2% and 43.1%, respectively) compared to the non-stimulated, control group (5.1%). The number of embryos destined for culture did not differ between the single and double synchronized groups. The highest percentage of hatched embryos was observed in the control group. In conclusion, the single synchronization and superovulation schedule is sufficient to obtain high numbers of embryos, however, both synchronization methods resulted in the recovery of considerable numbers of degenerated embryos. A higher number and percentage of hatched embryos after culture was found among embryos from the control group compared to gonadotropinstimulated gilts.

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Section: Discussionmentioning

confidence: 74%

Influence of Estrus Synchronization of Prepubertal Gilts on Embryo Quality

Ziȩcik

Biallowicz

Kaczmarek

et al. 2005

Journal of Reproduction and Development

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“…Wiesak et al (1990) reported a dissimilar pattern of follicular development in PMSG-and hCG-treated, immature gilts compared with naturally cycling pigs. Also, porcine zygote development in vitro was greater when zygotes were collected from mature gilts than when they were collected from prepubertal gilts (Pinkert et al, 1989).…”

Section: Discussionmentioning

confidence: 91%

In vitro development of zygotes from prepubertal gilts after microinjection of DNA

Williams¹,

Canseco²,

Knight³

et al. 1992

Journal of Animal Science

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ABSTRACT:The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and hCG. Zygotes received either pronuclear microinjection of buffer alone, buffer containing a DNA construct, or no microinjection. Zygotes were cultured in vitro in either modified Krebs-Ringer bicarbonate medium (KRBI for 144 h or in mouse oviduct (MO) explant culture with KRB for 48, 72, 96, .14, respectively). Microinjection of DNA, not merely the mechanical procedure, was detrimental to early zygote development and may be the cause of low pregnancy rates.

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“…However, it has been found that immature oocytes from cows with reduced reproductive performance or slaughtered at the end of their use are heterogeneous in quality [9]. The developmental capacity of oocytes from pre-pubertal animals was lower than that of oocytes from adult animals (cattle: [14][15][16][17]; sheep: [18,19]; pig: [20]; goat: [21,22]). Follicular waves and advanced atresia of the ovary also affected the developmental competence of oocytes [11,23].…”

Section: Introductionmentioning

confidence: 99%