In vitro determination of the antibiotic susceptibility of biofilm-forming Pseudomonas aeruginosa and Staphylococcus aureus: possible role of proteolytic activity and membrane lipopolysaccharide

Masadeh, Majed M; Mhaidat, Nizar M.; Alzoubi, Karem H.; Hussein, Emad I.; Al-Trad, Esra’a I.

doi:10.2147/idr.s41501

Cited by 29 publications

(28 citation statements)

References 36 publications

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“…Bacterial biofilms were prepared as described previously (Masadeh et al 2013;Cernohorska and Votava 2008). Briefly, 100 ll of bacterial suspension from each of the bacterial strains tested were cultivated in polypropylene tubes containing 2 ml of Trypticase Soy Broth (TSB) supplemented with 1 % glucose for 48 h at 37°C.…”

Section: Biofilm Formation and Screeningmentioning

confidence: 99%

Cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria

et al. 2014

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Metal oxide nanoparticles have been suggested as good candidates for the development of antibacterial agents. Cerium oxide (CeO 2 ) and iron oxide (Fe 2 O 3 ) nanoparticles have been utilized in a number of biomedical applications. Here, the antibacterial activity of CeO 2 and Fe 2 O 3 nanoparticles were evaluated on a panel of gram positive and gram negative bacteria in both the planktonic and biofilm cultures. Additionally, the effect of combining CeO 2 and Fe 2 O 3 nanoparticles with the broad spectrum antibiotic ciprofloxacin on tested bacteria was investigated. Thus, minimum inhibitory concentrations (MICs) of CeO 2 and Fe 2 O 3 nanoparticles that are required to inhibit bacterial planktonic growth and bacterial biofilm, were evaluated, and were compared to the MICs of the broad spectrum antibiotic ciprofloxacin alone or in the presence of CeO 2 and Fe 2 O 3 nanoparticles. Results of this study show that both CeO 2 and Fe 2 O 3 nanoparticles fail to inhibit bacterial growth and biofilm biomass for all the bacterial strains tested. Moreover, adding CeO 2 or Fe 2 O 3 nanoparticles to the broad spectrum antibiotic ciprofloxacin almost abolished its antibacterial activity. Results of this study suggest that CeO 2 and Fe 2 O 3 nanoparticles are not good candidates as antibacterial agents, and they could interfere with the activity of important antibiotics.

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Section: Biofilm Formation and Screeningmentioning

confidence: 99%

Cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria

et al. 2014

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“…However, the indiscriminate use of antibiotics resulted in the bacteria adaptation through the development of resistance 2 . Thus, pharmaceutical companies and researchers have devoted efforts to find out compounds free of resistance 3, 4 .…”

Section: Introductionmentioning

confidence: 99%

Defeating Bacterial Resistance and Preventing Mammalian Cells Toxicity Through Rational Design of Antibiotic-Functionalized Nanoparticles

Oliveira

Saito

Bido

et al. 2017

Sci Rep

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The rational synthesis of alternative materials is highly demanding due to the outbreak of infectious diseases and resistance to antibiotics. Herein, we report a tailored nanoantibiotic synthesis protocol where the antibiotic binding was optimized on the silver-silica core-shell nanoparticles surface to maximize biological responses. The obtained silver nanoparticles coated with mesoporous silica functionalized with ampicillin presented remarkable antimicrobial effects against susceptible and antibiotic-resistant Escherichia coli. In addition, these structures were not cell-death inducers and different steps of the mitotic cell cycle (prophase, anaphase and metaphase) were clearly identified. The superior biological results were attributed to a proper and tailored synthesis strategy.

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“…The biofilm shows maximum resistance to antibiotics in the mature stage due to several mechanisms [99,100] including:…”

Section: Biofilm and Bacterial Advantagesmentioning

confidence: 99%

Biofilm: An Important Bacterial Feature Still to Deal With

Novais

Abreu

Castro

2016

Journal of Life Sciences Research

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Currently, the bacterial infections are one of the main causes of death worldwide and a public health problem for most governments. In this scenario, virulence factors are of importance, including the capacity of forming biofilm. The ability of producing biofilms is directly involved in bacterial pathogenicity and hugely worse the risk of death due to a bacterial infection. This feature allows the bacteria to colonize different surfaces (e.g. Medical devices), and causes several complications, especially in nosocomial infections. This work reviewed biolfim producing mechanisms, the relation with resistance process and the problems associated to biofilms-affected-medical devices.

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In vitro determination of the antibiotic susceptibility of biofilm-forming Pseudomonas aeruginosa and Staphylococcus aureus: possible role of proteolytic activity and membrane lipopolysaccharide

Cited by 29 publications

References 36 publications

Cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria

Cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria

Defeating Bacterial Resistance and Preventing Mammalian Cells Toxicity Through Rational Design of Antibiotic-Functionalized Nanoparticles

Biofilm: An Important Bacterial Feature Still to Deal With

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