In vitro detection of cross-resistant and non-cross-resistant agents with fluorouracil for patients with colorectal cancer

Yamashita, Kazuki; Urakami, Atsushi; Kubozoe, Tadahiko; Ikeda, Masaharu; Hirabayashi, Yoko; Yamamura, Masatoshi; Iki, Katsumichi; Akiyoshi, Takashi; Matsumoto, Hideo; Hirai, Toshihiro; Sadahira, Yoshito; Tsunoda, Tsukasa

doi:10.1007/s10147-005-0509-6

Cited by 3 publications

(2 citation statements)

References 25 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Attempts of testing chemosensitivity of tumor cells ex vivo were already made in the eighties, but have not developed into a standard chemosensitivity test so far (Selby and Raghaven 1981 ). Recently, research groups have established a link between clinical responses to chemotherapeutics and ex vivo chemosensitivity analysis (Nakada et al 2005 ; Yamashita et al 2005 ; Trojan et al 2005 ). Moreover DNA microarrays (Gianni et al 2005 ) and multiparametric microsensor chips (Otto et al 2003 ) have been developed for chemosensitivity testing.…”

Section: Introductionmentioning

confidence: 99%

Viability analysis and apoptosis induction of breast cancer cells in a microfluidic device: effect of cytostatic drugs

Komen

Wolbers

Franke

et al. 2008

Biomed Microdevices

View full text Add to dashboard Cite

Breast cancer is the leading cause of cancer deaths among non-smoking women worldwide. At the moment the treatment regime is such that patients receive different chemotherapeutic and/or hormonal treatments dependent on the hormone receptor status, the menopausal status and age. However, in vitro sensitivity testing of tumor biopsies could rationalize and improve the choice of chemo-and hormone therapy. Lab-on-a-Chip devices, using microfluidic techniques, make detailed cellular analysis possible using fewer cells, enabling working with a patients' own cells and performing chemo-and hormone sensitivity testing in an ex vivo setting. This article describes the development of two microfluidic devices made in poly(dimethylsiloxane) (PDMS) to validate the cell culture properties and analyze the chemosensitivity of MCF-7 cells (estrogen receptor positive human breast cancer cells) in response to the drug staurosporine (SSP). In both cases, cell viability was assessed using the life-stain Calcein-AM (CAAM) and the death dye propidium iodide (PI). MCF-7 cells could be statically cultured for up to 7 days in the microfluidic chip. A 30 min flow with SSP and a subsequent 24 h static incubation in the incubator induced apoptosis in MCF-7 cells, as shown by a disappearance of the aggregate-like morphology, a decrease in CAAM staining and an increase in PI staining. This work provides valuable leads to develop a microfluidic chip to test the chemosensitivity of tumor cells in response to therapeutics and in this way improve cancer treatment towards personalized medicine.

show abstract

Section: Introductionmentioning

confidence: 99%

Viability analysis and apoptosis induction of breast cancer cells in a microfluidic device: effect of cytostatic drugs

Komen

Wolbers

Franke

et al. 2008

Biomed Microdevices

View full text Add to dashboard Cite

show abstract

“…The combined regimens were FX with bevacizumab or cetuximab, and FR with bevacizumab or cetuximab. The doses initially used were based on the usual clinical dose as determined from pharmacokinetic and pharmacodynamic parameters obtained through phase I studies and empirical assays (8,9). Median inhibitory concentrations (IC 50 ) of tested drugs were determined as those causing a 50% dose-dependent reduction in cell viability in complete growth medium after 48 h of continuous exposure to the drug.…”

mentioning

confidence: 99%

Development and Applicability of Integrative Tumor Response Assays for Metastatic Colorectal Cancer

Yoon

Kim

Roh

et al. 2017

View full text Add to dashboard Cite

show abstract

Evaluation of novel histone deacetylase inhibitors as therapeutic agents for colorectal adenocarcinomas compared to established regimens with the histoculture drug response assay

Kim

Lee

et al. 2008

Int J Colorectal Dis

View full text Add to dashboard Cite

show abstract

In vitro detection of cross-resistant and non-cross-resistant agents with fluorouracil for patients with colorectal cancer

Abstract: Only irinotecan exhibited a spectrum independent of that of 5-FU, thus indicating that it could be an appropriate agent for second-line chemotherapy after fluorouracil failure.

Cited by 3 publications

References 25 publications

Viability analysis and apoptosis induction of breast cancer cells in a microfluidic device: effect of cytostatic drugs

Viability analysis and apoptosis induction of breast cancer cells in a microfluidic device: effect of cytostatic drugs

Development and Applicability of Integrative Tumor Response Assays for Metastatic Colorectal Cancer

Evaluation of novel histone deacetylase inhibitors as therapeutic agents for colorectal adenocarcinomas compared to established regimens with the histoculture drug response assay

Contact Info

Product

Resources

About