In Vitro Delivery and Controlled Release of Doxorubicin for Targeting Osteosarcoma Bone Cancer

Abstract

Drug delivery systems are designed to achieve drug therapeutic index and enhance the efficacy of controlled drug release targeting with specificity and selectivity by successful delivery of therapeutic agents at the desired sites without affecting the non-diseased neighbouring cells or tissues. In this research, we developed and demonstrated a bio-based calcium carbonate nanocrystals carrier that can be loaded with anticancer drug and selectively deliver it to cancer cells with high specificity by achieving the effective osteosarcoma cancer cell death without inducing specific toxicity. The results showed pH sensitivity of the controlled release characteristics of the drug at normal physiological pH 7.4 with approximately 80% released within 1,200 min but when exposed pH 4.8 the corresponding 80% was released in 50 min. This study showed that the DOX-loaded CaCO 3 nanocrystals have promising applications in delivery of anticancer drugs.

Synthesis and characterization of a multifunctional gold-doxorubicin nanoparticle system for pH triggered intracellular anticancer drug release

“…It has been reported that PEGylated dendrimer nanoparticles are internalized into the cells by an EPR effect and reside within the cell outside the nucleus most likely in the lysosomal compartments of the cell [45]. In the study here free doxorubicin and DOX conjugated nanoparticles showed different cellular uptake mechanisms of the drug by the cells [46]. As can be seen in Fig.…”

Physicochemical characterization and antimicrobial evaluation of gentamicin-loaded CaCO3 nanoparticles prepared via microemulsion method

“…The drug loading and drug entrapment efficiency, important factors for a drug delivery system, are used to evaluate the usability of nano-based carriers [49]. Drug loading and loading efficiency of the drug-loaded CaCO 3 nanoparticles were calculated 25.3± 2.5 and 38.6±1.2 %, respectively.…”

Development of nanoantibiotic delivery system using cockle shell-derived aragonite nanoparticles for treatment of osteomyelitis

“…[39][40][41] Based on the results of cytotoxicity assay, VANP is biocompatible and thus suitable for the development of a nanoantibiotic …”