In vitro degradation of calcium phosphates: Effect of multiscale porosity, textural properties and composition

Díez-Escudero, Anna; Español, Montserrat; Beats, S.; Ginebra, Maria‐Pau

doi:10.1016/j.actbio.2017.07.033

Cited by 65 publications

(58 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…7). The high degradation rate of CDHA, compared particularly to the more soluble β-TCP, agrees with previous studies 65 and can be explained by its specific features, i.e., the nanosized crystals and consequently high SSA, as well as its low crystallinity and lack of stoichiometry. The degradation of the BCP-Foam, which presented a high amount of ectopic bone formation at 12 weeks, was significantly smaller.…”

Section: Degradation Patterns and Osteoinductionsupporting

confidence: 90%

Osteoinduction by Foamed and 3D-Printed Calcium Phosphate Scaffolds: Effect of Nanostructure and Pore Architecture

Barba

Díez-Escudero

Maazouz

et al. 2017

ACS Appl. Mater. Interfaces

155

167

View full text Add to dashboard Cite

Some biomaterials are osteoinductive, that is, they are able to trigger the osteogenic process by inducing the differentiation of mesenchymal stem cells to the osteogenic lineage. Although the underlying mechanism is still unclear, microporosity and specific surface area (SSA) have been identified as critical factors in material-associated osteoinduction. However, only sintered ceramics, which have a limited range of porosities and SSA, have been analyzed so far. In this work, we were able to extend these ranges to the nanoscale, through the foaming and 3D-printing of biomimetic calcium phosphates, thereby obtaining scaffolds with controlled micro- and nanoporosity and with tailored macropore architectures. Calcium-deficient hydroxyapatite (CDHA) scaffolds were evaluated after 6 and 12 weeks in an ectopic-implantation canine model and compared with two sintered ceramics, biphasic calcium phosphate and β-tricalcium phosphate. Only foams with spherical, concave macropores and not 3D-printed scaffolds with convex, prismatic macropores induced significant ectopic bone formation. Among them, biomimetic nanostructured CDHA produced the highest incidence of ectopic bone and accelerated bone formation when compared with conventional microstructured sintered calcium phosphates with the same macropore architecture. Moreover, they exhibited different bone formation patterns; in CDHA foams, the new ectopic bone progressively replaced the scaffold, whereas in sintered biphasic calcium phosphate scaffolds, bone was deposited on the surface of the material, progressively filling the pore space. In conclusion, this study demonstrates that the high reactivity of nanostructured biomimetic CDHA combined with a spherical, concave macroporosity allows the pushing of the osteoinduction potential beyond the limits of microstructured calcium phosphate ceramics.

show abstract

Section: Degradation Patterns and Osteoinductionsupporting

confidence: 90%

Osteoinduction by Foamed and 3D-Printed Calcium Phosphate Scaffolds: Effect of Nanostructure and Pore Architecture

Barba

Díez-Escudero

Maazouz

et al. 2017

ACS Appl. Mater. Interfaces

155

167

View full text Add to dashboard Cite

show abstract

“…The SSA and porosity of materials used in current study was described in previous reports. [71] Cell culture study: The protocol of isolation of rat mesenchymal stem cells (rMSCs) and rat endothelial progenitor cells (rEPCs) was described elsewhere. [72] Briefly, cells were obtained from the tibias and femurs of Lewis rats at the Institute for Bioengineering of Catalonia (IBEC).…”

Section: Methodsmentioning

confidence: 99%

“…The vertical lines represent the patterns of HA (JCPDS 82-1943), α-TCP (JCPDS 09-0348) and β-TCP (JCPDS 70-2065) from the Joint Committee on powder Diffraction Standards. D) Valuesof roughness, specific surface area (SSA) and total porosity [71].…”

mentioning

confidence: 99%

The Influence of Physicochemical Properties of Biomimetic Hydroxyapatite on the In Vitro Behavior of Endothelial Progenitor Cells and Their Interaction with Mesenchymal Stem Cells

Sadowska

Guillem-Marti

Ginebra

2018

Adv Healthcare Materials

View full text Add to dashboard Cite

Calcium phosphate (CaP) substrates are successfully used as a bone grafts due to their osteogenic properties. However, the influence of the physicochemical features of CaPs in angiogenesis is frequently neglected despite it is a crucial process for bone regeneration. The present work focuses on analyzing the effect of textural parameters of biomimetic calcium deficient hydroxyapatite (CDHA) and sintered beta-tricalcium phosphate (β-TCP) such as specific surface area (SSA), surface roughness and microstructure on the behavior of rat endothelial progenitor cells (rEPCs) and their crosstalk with rat mesenchymal stem cells (rMSCs). The higher reactivity of CDHA resulted in low proliferation rates in mono-and cocultured systems. This effect was especially pronounced for rMSCs alone, and for CDHA Revised Manuscript with fine microstructure. In terms of angiogenic and osteogenic gene expression, the upregulation of particular genes was especially enhanced for needle-like CDHA compared to plate-like CDHA and β-TCP suggesting the importance not only of chemistry of substrate but also its textural features. Moreover, the coculture of rEPCs and rMSCs on needle-like CDHA resulted in early upregulation of osteogenic modulator i.e. protein deglycase 1 (DJ 1) what might be a possible cause of overexpression of osteogenic-related genes on the same substrate.

show abstract

“…The expression of osteoblastic specific markers by rMSCs cultured on the CDHA and β-TCP small discs was performed using RT-qPCR ( The extent of this ionic exchange depends on the intrinsic biomaterial properties. For instance, in the case of biomimetic calcium deficient hydroxyapatite, the deficiency in calcium, low crystallinity and SSA lead to a highly reactive material, which can be even more soluble than sintered β-TCP [32] and displays a high bioactivity and osteoinductive properties [17]. These features, which are highly beneficial in the in vivo scenario, may however introduce a high level of complexity when looking at the in vitro cell response to these materials.…”

Section: Gene Expressionmentioning

confidence: 99%

In vitro response of mesenchymal stem cells to biomimetic hydroxyapatite substrates: A new strategy to assess the effect of ion exchange

et al. 2018

View full text Add to dashboard Cite

Fluid mediated interactions play a significant role in the bioactivity of calcium phosphates. Ionic exchange is amplified in the case of biomimetic hydroxyapatite, which makes the in vitro characterisation of cell-material interactions especially challenging. The present work proposes a novel and simple strategy to explore the mechanisms of interaction of biomimetic and sintered calcium phosphates with mesenchymal stem cells. The effects of topography and ion exchange are analysed separately by modifying the volume ratio between cell culture medium and biomaterial. High ionic fluctuations interfered in the maturation of focal adhesions, hampering cell adhesion and leading to increased apoptosis and reduced proliferation rate.

show abstract

In vitro degradation of calcium phosphates: Effect of multiscale porosity, textural properties and composition

Cited by 65 publications

References 58 publications

Osteoinduction by Foamed and 3D-Printed Calcium Phosphate Scaffolds: Effect of Nanostructure and Pore Architecture

Osteoinduction by Foamed and 3D-Printed Calcium Phosphate Scaffolds: Effect of Nanostructure and Pore Architecture

The Influence of Physicochemical Properties of Biomimetic Hydroxyapatite on the In Vitro Behavior of Endothelial Progenitor Cells and Their Interaction with Mesenchymal Stem Cells

In vitro response of mesenchymal stem cells to biomimetic hydroxyapatite substrates: A new strategy to assess the effect of ion exchange

Contact Info

Product

Resources

About