In vitro decidualization of rat endometrial stromal cells

Matsumoto, Kenji; Yamauchi, Nobuhiko; Watanabe, Ryoji; Oozono, Shinji; Kubota, Kaiyu; Nishimura, Kyohei; Wood, Chris; Soh, Tomoki; Kizaki, Kei Ichirou; Hattori, Masa Aki

doi:10.1007/s00441-008-0734-1

Cited by 22 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preparation and Culture of the UESCs UESCs were isolated from Per2-dLuc transgenic rats on day 4.50 and day 6.50 of gestation as reported previously (He et al, 2007b;Matsumoto et al, 2009;Oozono et al, 2008). The uterine lumens were filled with phosphatebuffered saline (PBS) containing 0.1% collagenase and incubated at 37.8°C for 1 h in a shaking water bath.…”

Section: Animalsmentioning

confidence: 99%

Down-Regulation of Circadian Clock GenePeriod 2in Uterine Endometrial Stromal Cells of Pregnant Rats During Decidualization

Uchikawa

Kawamura

Yamauchi

et al. 2010

Chronobiology International

Self Cite

View full text Add to dashboard Cite

Circadian rhythms are modulated in a variety of peripheral tissues, including in the uterus where endometrial stromal cells (UESCs) undergo proliferation and differentiation (decidualization) during gestation. Here the authors focused on circadian rhythms in UESCs during implantation and decidualization in rodents. As revealed by analyses of cultured UESCs from pregnant Per2 promoter-dLuc transgenic rats, Per2 oscillation of ∼24 h was observed in response to dexamethasone. Per2 oscillation was enhanced in UESCs during implantation, whereas they were attenuated during decidualization. In vivo studies showed that PER2 protein in the uteri displayed a peak at zeitberger time 4 (ZT 4) (day 4.50 of gestation) and a trough at ZT 12 (day 4.83), indicating its circadian rhythmicity. Conversely, no significant circadian rhythm of the PER2 protein was observed during decidualization. Fluorescent immunohistochemical studies also supported circadian rhythmicity of the PER2 protein in its intracellular distribution. In accordance with Per2 mRNA expression, a circadian rhythm of vascular endothelial growth factor (Vegf) gene expression, having several E-box or E-box-like sites at the upstream of the transcription start site, was observed during implantation, showing a peak at ZT 0 and a trough at ZT 12. In contrast, Vegf mRNA expression displayed no circadian rhythm during decidualization. Collectively, the present results prove that Per2 oscillation is down-regulated in UESCs during decidualization. It is strongly suggested that cellular differentiation in UESCs interferes with circadian clockwork.

show abstract

Section: Animalsmentioning

confidence: 99%

Down-Regulation of Circadian Clock GenePeriod 2in Uterine Endometrial Stromal Cells of Pregnant Rats During Decidualization

Uchikawa

Kawamura

Yamauchi

et al. 2010

Chronobiology International

Self Cite

View full text Add to dashboard Cite

show abstract

“…As progesterone does not cause an immediate decidualization reaction, in vivo or in vitro, there must be intermediate or other signals involves. It is known, e.g., that the second messenger cAMP is essential for inducing and maintaining differentiation of human and rodent ESCs in vitro [36,37]. cAMP, which is generated upon ligand binding to a G-protein coupled receptor (GPCR), is elevated in human endometrial tissue from the secretory relative to proliferative phase of the menstrual cycle [38].…”

Section: How Did Spontaneous Decidualization Evovle?mentioning

confidence: 99%

The evolution of menstruation: A new model for genetic assimilation

Emera¹,

Romero²,

Wagner³

2011

BioEssays

146

View full text Add to dashboard Cite

Why do humans menstruate while most mammals do not? Here, we present our answer to this long-debated question, arguing that (i) menstruation occurs as a mechanistic consequence of hormone-induced differentiation of the endometrium (referred to as spontaneous decidualization, or SD); (ii) SD evolved because of maternal-fetal conflict; and (iii) SD evolved by genetic assimilation of the decidualization reaction, which is induced by the fetus in non-menstruating species. The idea that menstruation occurs as a consequence of SD has been proposed in the past, but here we present a novel hypothesis on how SD evolved. We argue that decidualization became genetically stabilized in menstruating lineages, allowing females to prepare for pregnancy without any signal from the fetus. We present three models for the evolution of SD by genetic assimilation, based on recent advances in our understanding of the mechanisms of endometrial differentiation and implantation. Testing these models will ultimately shed light on the evolutionary significance of menstruation, as well as on the etiology of human reproductive disorders like endometriosis and recurrent pregnancy loss.

show abstract

“…While the marker was expressed in decidua from 8dpc, we did not detect dPrl in UIII cells after 4 days in culture with 10% DCC (data not shown). The expression of dPrl has been reported in response to medroxyprogesterone acetate (MPA), but not in cells decidualized under influence of progesterone and estradiol (Matsumoto, 2009). There is evidence that dPrl appeared at later time points, in presence of FBS (Prigent-Tessier et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

Vallejo

Maschi

Mestre-Citrinovitz

et al. 2009

Journal Cellular Physiology

View full text Add to dashboard Cite

During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3 fold, FDR > 0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.

show abstract

In vitro decidualization of rat endometrial stromal cells

Cited by 22 publications

References 47 publications

Down-Regulation of Circadian Clock GenePeriod 2in Uterine Endometrial Stromal Cells of Pregnant Rats During Decidualization

Down-Regulation of Circadian Clock GenePeriod 2in Uterine Endometrial Stromal Cells of Pregnant Rats During Decidualization

The evolution of menstruation: A new model for genetic assimilation

Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

Contact Info

Product

Resources

About