In vitro cytotoxicity of galvanically coupled magnesium‐titanium particles on human osteosarcoma SAOS2 cells: A potential cancer therapy

Abstract

Osteosarcoma is a malignant bone cancer that occurs mostly in children and young adults. This study investigated the cytotoxicity of Mg and Mg-Ti microparticles to human osteosarcoma cells. Osteosarcoma cells were killed in a dosage-dependent manner when cells, with a cell seeding density of 30,000 cells/cm , were cultured with 0 to 2500 µg/mL of Mg or Mg-Ti in cell culture media for 24-72 h. Mg-Ti killed cells more effectively, where 1250 µg/mL of Mg-Ti killed cells completely by 24 h, while 2500 µg/mL of Mg killed nearly all cells, but not all. Killing due to particle corrosion occurred mostly during the first 24 h, and so the percent cell viability between 24 and 72 h showed not much variability. However, the measurement of live and dead cell numbers, over the timeframe of 24-72 h, showed more insight, such as cell recovery. If particle concentrations were low, the number of live cells increased after 24 h, indicating cell proliferation. If particle concentrations were high, the number of live cells either remained steady or decreased, indicating cell quiescence or continued killing, respectively. Increase in the number of dead cells also indicated killing, while plateau meant discontinued killing. In addition, repeated killing of recovered cells exhibited the same dose-dependent killing profile as the initial experiment, implying little development of cell resistance to treatment. These results, together, show that osteosarcoma cells are susceptible to killing by way of exposure to corroding particles, showing highly effective killing using the galvanic couple of Mg-Ti. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

Nontribological corrosion modes dominate wrought CoCrMo acetabular taper corrosion: A retrieval study

“…Bijukumar et al in a recent study also indicate the presence of similar features with a similar size distribution within head-neck tapers on retrieved devices. 41 In future work, retrieved devices with well-preserved biological material can be analyzed through sputter-coated SEM analysis 42 or Fourier transform infra-red spectroscopy (FTIR). 43 Scharf et al published findings from periprosthetic retrieved tissue histology that show corrosion debris phagocytosed by a macrophage cell.…”

The Effect of Equal-Channel Angular Pressing on the Microstructure, the Mechanical and Corrosion Properties and the Anti-Tumor Activity of Magnesium Alloyed with Silver

“…Most of these works aim at the use of magnesium alloys in orthopedics and cardiovascular surgery. However, studies are becoming increasingly popular in which the ability of magnesium alloys to suppress the growth of tumor cells is scrutinized [7,8,9,10]. This opens up new possibilities for creating orthopedic implants or products with anti-tumor activity for insertion directly into the tumor area [11].…”

Two Modes of Toxicity of Lipid Nanoparticles Containing a pH-Sensitive Cationic Lipid on Human A375 and A375-SM Melanoma Cell Lines

“…2 indicates that the dose-dependent toxicity caused by YSK05-LNPS, as both PEG-DMG YSK05-LNPs and PEG-DSG YSK05-LNPs caused similar toxicities at higher concentrations (80 µM and higher) while at lower concentrations, no significant toxicity was observed. This typical toxicity pattern 15,16,18,22,25,[27][28][29] was similar for both A375 and A375-SM cell lines. However, a longer exposure time (up to 72 h) allowed lower concentrations (<80 µM) to overcome this dividing point and to trigger toxicity as well (Figs.…”

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