In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract

Okur, Mehmet Evren; Karakaş, Nihal; Karadağ, Aye Esra; Yılmaz, Rabia; Demirci, Fatih

doi:10.12991/jrp.2019.180

Cited by 11 publications

(12 citation statements)

References 14 publications

(15 reference statements)

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“…In addition, the methanolic extract of M. vulgare was evaluated for its in vitro cytotoxic activity by measuring the percentage of viable cells’ glioblastoma multiforme cell lines (U87, LN229 and T98G) using a luminescence system. After evaluating the cytotoxicity impact that M. vulgare has on U87 (IC 50 : 270.3 μM), LN229 (IC 50 : 343 μM), and T98G (IC 50 : 336.6 μM) from glioblastoma multiforme cell lines, it was concluded that it is the most efficient in two cell lines, U87 (69.9%) and LN229 (71%) [ 30 ].…”

Section: Applications In Medicine and As Pesticidesmentioning

confidence: 99%

“…Aqueous extract 175.00 mg GAE/100 g dry material 23.86 mg QE/100 g dry material [26] Aqueous infusion na 5.08 mg/100 mg dry material [27] Ethanol-water extract (70:30, v/v) 93.42 mg of GAE 23.25 mg of RUE/g of extract [14] Ethanol-water extract (70:30, v/v) 59.87 mg GAE/g dry extract 14.47 mg QE/g dry extract [28] Methanol extract (dry) 44.89 mg GAE/g dry extract 24.60 mg QE/g dry extract [29] Methanol extract 48. .00 mg GAE/g extract 12.05-93.12 mg QE/100 g extract [25,30,31] Microwave assisted extract 61.44 mg of GAE/g of extract 37.70 mg of RUE/g of extract [14] Subcritical…”

Section: Phenolic Content Flavonoid Content Referencesmentioning

confidence: 99%

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Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

et al. 2020

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Marrubium vulgare is a plant with high bioactive potential. It contains marrubiin, a labdane diterpene that is characteristic for this genus, as well as a complex mixture of phenolic compounds. According to numerous studies, M. vulgare acts as a good antioxidant agent, and due to this, it could potentially be useful in treatments of cancer, diabetes mellitus, and liver diseases. In addition, its anti-inflammatory, wound-healing, antihypertensive, hypolipidemic, and sedative potential are discussed. Apart from that, its antimicrobial activity, especially against Gram+ bacteria, fungi, herpes simplex virus, and parasites such as Toxoplasma gondii, Trichomonas vaginalis, and Plasmodium berghei-berghei was recorded. Additionally, it could be used as a chicken lice repellent, herbicide, and natural insecticide against mosquito larvae and natural molluscicide. In veterinary medicine, M. vulgare can be used as an anthelmintic against the eggs and larvae of bovine strongyles parasites, and as an antibiotic against bovine mastitis caused by resistant bacterial strains. Due to the mentioned benefits, there is a tendency for the cultivation of M. vulgare in order to ensure high-quality raw material, but more firm scientific evidence and well-designed clinical trials are necessary for the well-established use of M. vulgare herb and its preparations.

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Section: Applications In Medicine and As Pesticidesmentioning

confidence: 99%

Section: Phenolic Content Flavonoid Content Referencesmentioning

confidence: 99%

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

et al. 2020

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“…The means of phenolic and flavonoids contents recorded for Moroccan M. vulgare extract were 60.409 mg GAE/g and 33.81 mg CE/g, respectively [ 20 ]. Okur et al [ 21 ] signaled that the German extract showed 48.97 ± 0.82 mg GA/g corresponding to the total phenolic amounts. In the same way, Ouchemoukh et al [ 22 ] showed that the Algerian M. vulgare exhibited an important content of total phenolics and flavonoid, equivalent to 40 mg GAE/g and 10 mg/g, respectively, whereas Amri et al [ 7 ] reported that the M. vulgare extract was richer in flavonoid (45.21 mg/g) than in polyphenols (6 mg GAE/g).…”

Section: Resultsmentioning

confidence: 99%

“…For example, the IC 50 of Tunisian M. vulgare extract in the presence of DPPH was 35 µg/mL and 38 µg/mL reported by Aouadhi et al [ 8 ] and Amri et al [ 7 ], respectively. In addition, Bouterfas et al [ 25 ] showed wide ranges of IC 50 (DPPH) varying from 33.7 to 124 µg/mL of Algerian M. vulgare methanolic extract collected from three sites, while the lowest antioxidant power was observed in Moroccan and German M. vulgare methanoIic extracts where the IC 50 was 2.4 mg/L and 0.8 mg/L and 2.08 and 1.33 mg/mL in the presence of DPPH and ABTS, respectively [ 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi Marrubium vulgare against Foodborne Diseases Bacteria

2021

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The present study aims to evaluate the chemical composition, metabolites secondary and pharmacology activities of methanolic extract of Marrubium vulgare collected from King Saudi Arabia. Moreover, the primary mode of action of the tested extract was studied here for the first time against E. coli and L. monocytogenes. HPLC analysis shows that the major components in the tested extract are luteolin-7-O-d-glucoside, ferulic acid and premarrubiin. Obtained data demonstrated that the investigated extract was richer in phenol (26.8 ± 0.01 mg/GAE g) than in flavonoids (0.61 ± 0.05 mg EC/mL). In addition, the methanolic extract showed an important antioxidant capacity against the DPPH (IC50 = 35 ± 0.01 µg/mL) and ABTS (IC50 = 25 ± 0.2 µg/mL) radical scavenging and a strong inhibition of acetylcholinesterase enzyme with an IC50 value corresponding to 0.4 mg/mL. The antibacterial activity demonstrated that the evaluated extract had significant activity against both Gram-positive and Gram-negative bacteria. The effect of time on cell integrity on E. coli and L. monocytogenes determined by time–kill and bacteriolysis tests showed that the M. vulgare extract reduced the viability of both strains after 8 and 10 h and had a bacteriolytic effect against two different categories of bacteria, Gram-positive and negative, which are not of the same potency. Based on obtained data, it can be concluded that Saudi M. vulgare has a high pharmacological importance and can be used in preparation of food or drugs.

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“…The evaluation of the anti-cancer activity of plant extracts is essential for safe treatment. Various anti-cancer activity studies with cell lines used in this study are available on plant extracts (Okur et al 2019). OFI fruits are also in the safe range in terms of toxicity because they are consumed as food among the public.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract

Okur¹,

Karakaş²,

Karadağ³

et al. 2019

Istanbul J Pharm

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Opuntia ficus-indica (L.) Miller (Prickly pear cactus) is located in the Mediterranean and Aegean Region and belongs to the Cactaceae family (Kabas et al. 2006). This species is known as "kaynana dili, firenk inciri, hint inciri" in Turkey (Güner et al. 2012) and as "prickly pear" elsewhere in the world. The nutritional properties of this species have long been known. In Mediterranean countries, cladodes are not typically consumed as nutrients, but the fruits are largely consumed due to natural antioxidants which are may be protective against oxidative damage (Lee et al. 2002). The fruits are used also in traditional medicine for their hypolipidemic and hypoglycemic actions (Butera et al. 2002). The phytochemical compounds of prickly pear fruits and cladodes are vitamins, carotenoids, betalains, and polyphenolic compounds which have proven biological activities and health benefits (anti-cancer, anti-diabetic, anti-inflammatory, neuroprotective effect) (Ammar et al. 2018; Mena et al. 2018). OFI is especially rich in flavonoids and phenolic compounds. Due to the high amount of these substances the prickly pear fruit has a potent antioxidant activity (Butera et al. 2002; Lee et al. 2002; Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.

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In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract

Cited by 11 publications

References 14 publications

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi Marrubium vulgare against Foodborne Diseases Bacteria

Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract

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