In Vitro Cytotoxicity Evaluation of a Self-adhesive, Methacrylate Resin–based Root Canal Sealer

Pinna, Lorenzo A.; Brackett, Martha Goël; Lockwood, Petra E.; Huffman, Bradford; Mai, Sui; Cotti, Elisabetta; Dettori, Claudia; Pashley, David H.; Tay, Franklin R.

doi:10.1016/j.joen.2008.06.020

Cited by 49 publications

(74 citation statements)

References 30 publications

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“…Additionally, it is a low-cost procedure that yields results within 48 h (12,13,18,19), and is therefore the most commonly used procedure for determining the cytotoxicity of different types of materials (20). Due to these characteristics and the endorsement by some authors (13,15,(21)(22)(23)(24)(25), this assay was used in this study for assessing the cytotoxicity of all the six endodontic sealers.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. The MTT assay was used for analysis of cell viability. Twelve specimens of each sealer were prepared and randomly assigned to 6 groups according to the commercial brands (n=4/time). A control group was also formed, which was not subjected to the contact with sealers. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO 2 and 100% humidity. MTT assays were performed in quadruplicate after 12, 24 and 72 h of contact of the sealer specimens with monolayers. Statistical analysis was performed by two-way ANOVA with Bonferroni post-hoc test at a significance level of 5%. Analysis of absorbance in the experimental groups showed that GuttaFlow presented the lowest cytotoxicity, with a mean absorbance of 0.048, followed by Pulp Canal Sealer (0.038), Sealer 26 (0.038), Endo Densell (0.036) and Pulp Fill (0.035). The control group had a mean absorbance of 0.098. Based on the results, Endofill and GuttaFlow were the most and the least cytotoxic sealers, respectively.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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“…11,24,25 The assumption of this strategy was that eluted components from the materials would change with time; previous studies with other materials suggest that elution from sealers decreases or stays the same with time. 11,28 The major goals of endodontic therapy are to remove infected soft and hard tissues and to seal the canal space against ingress of bacteria. These goals are in place because of reports that failure of endodontic therapy is often caused by residual or invading bacteria that trigger inflammation and tissue destruction via secreted toxins including lipopolysaccharide (LPS).…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of monocytic cytokine secretion by endodontic sealers

et al. 2011

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Recent studies have reported that sealers may alter the secretion of specific cytokines from THP1 monocytic cells in vitro. In this study, a cytokine array was used to determine if endodontic sealers changed secretion of 42 cytokines. White mineral trioxide aggregate (WMTA), MTA preparation (CS), AH-Plus (AHP), and Pulp Canal Sealer (PCS) were mixed, allowed to set for 72 h, then "aged" in buffered-saline for 12 weeks. Aged specimens were placed in direct contact with THP1 for 72 h and their cytotoxicity (MTT assay) was assessed. Materials that were not severely toxic were then exposed to THP1 with or without lipolysaccharide (LPS), and the culture medium was assayed for cytokine secretion. Secretion of cytokines was quantified using infrared scanning (Odyssey(®)); replicate pairs were averaged. PCS severely suppressed MTT activity and was not assessed for its influence on cytokine secretion. WMTA, CS, and AHP induced a broad-based increase in cytokine secretion (>20% vs. Teflon controls), but AHP induced the greatest increase (>100% in 17 of 42 cytokines). The effects of the sealers on LPS-activated THP1 were biphasic, with some increases and decreases cytokine secretion of >20%, but few larger effects. This work shows endodontic sealers may alter the secretion of a broad cross section of cytokines from monocytic cells.

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“…Numerous studies have been conducted to assess the biocompatibility of AH Plus but have yielded conflicting results. On the one hand, some in vitro studies showed that the cytotoxic effects of AH Plus were milder than those of zinc oxide-eugenol and methacrylate resin-based sealers, and were detectable only during the early period after mixing [15][16][17][18] . In vivo subcutaneous implantation studies also showed that AH Plus caused tissue irritation only during the initial post-implantation period 4,6) .…”

Section: Introductionmentioning

confidence: 99%

“…When compared with AH Plus, MetaSEAL reportedly demonstrated comparable sealing performance 25) . In terms of biocompatibility, in vitro studies showed that the cytotoxicity of MetaSEAL was initially higher than that of AH Plus but decreased over time to a comparable level 17,18) . Our recent in vivo study also showed that MetaSEAL induced a milder neutrophil infiltration in rat subcutaneous tissue than a zinc oxide-eugenol sealer 8) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the responses of MHC class II molecule-expressing cells and macrophages to epoxy resin-based and 4-META-containing, methacrylate resin-based root canal sealers in rat subcutaneous tissue

et al. 2013

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Major histocompatibility complex (MHC) class II molecule-expressing cells and macrophages play a pivotal role in mediating the host tissue response to biomaterials. This study investigated the responses of these cells to epoxy resin-based and 4-META-containing, methacrylate resin-based endodontic sealers (AH Plus and MetaSEAL respectively) in rat connective tissue. Silicone tubes loaded with one of the sealers or solid silicone rods (control) were subcutaneously implanted in male Wistar rats for three time periods of 7, 14, or 28 days. Tissue specimens were immunoperoxidase-stained for MHC class II molecules and CD68 (a general macrophage marker). Results showed that AH Plus-implanted tissue displayed significantly more MHC class II-positive cells than the control at 14 and 28 days, whereas MetaSEAL-implanted tissue showed significantly more CD68-positive cells than both AH Plus-implanted tissue and the control at all time periods. It was concluded that the epoxy resin-based sealer induced the infiltration of MHC class II molecule-expressing cells, whereas 4-META-containing, methacrylate resin-based sealer elicited macrophage infiltration.

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In Vitro Cytotoxicity Evaluation of a Self-adhesive, Methacrylate Resin–based Root Canal Sealer

Cited by 49 publications

References 30 publications

Cytotoxicity of root canal sealers on endothelial cell cultures

Cytotoxicity of root canal sealers on endothelial cell cultures

Dysregulation of monocytic cytokine secretion by endodontic sealers

Evaluation of the responses of MHC class II molecule-expressing cells and macrophages to epoxy resin-based and 4-META-containing, methacrylate resin-based root canal sealers in rat subcutaneous tissue

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