In Vitro Cytotoxicity Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

Pinho, Ana Rita; Martins, Filipa; Costa, Maria Elisabete V.; Senos, A.M.R.; Silva, Edgar F. da Cruz e; Pereira, Maria de Lourdes; Rebelo, Sandra

doi:10.3390/cells9051081

Cited by 47 publications

(35 citation statements)

References 81 publications

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“…Several researchers observed increased lipid peroxides in the cells, giving rise to more free radicals and damaged biomolecules such as DNA and other biostructures in the cell. They observed dose- and exposure time-dependent manners, where higher concentrations and prolonged exposure induced cell death with the loss of cell metabolism and membrane integrity [ 70 , 71 , 72 ].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Effect of Chitosan Films on Food Spoilage Bacteria

Wrońska

Katir

Miłowska

et al. 2021

IJMS

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Synthetic materials commonly used in the packaging industry generate a considerable amount of waste each year. Chitosan is a promising feedstock for the production of functional biomaterials. From a biological point of view, chitosan is very attractive for food packaging. The purposes of this study were to evaluate the antibacterial activity of a set of chitosan-metal oxide films and different chitosan-modified graphene (oxide) films against two foodborne pathogens: Campylobacter jejuni ATCC 33560 and Listeria monocytogenes 19115. Moreover, we wanted to check whether the incorporation of antimicrobial constituents such as TiO2, ZnO, Fe2O3, Ag, and graphene oxide (GO) into the polymer matrices can improve the antibacterial properties of these nanocomposite films. Finally, this research helps elucidate the interactions of these materials with eukaryotic cells. All chitosan-metal oxide films and chitosan-modified graphene (oxide) films displayed improved antibacterial (C. jejuni ATCC 33560 and L. monocytogenes 19115) properties compared to native chitosan films. The CS-ZnO films had excellent antibacterial activity towards L. monocytogenes (90% growth inhibition). Moreover, graphene-based chitosan films caused high inhibition of both tested strains. Chitosan films with graphene (GO, GOP, GOP-HMDS, rGO, GO-HMDS, rGOP), titanium dioxide (CS-TiO2 20:1a, CS-TiO2 20:1b, CS-TiO2 2:1, CS-TiO2 1:1a, CS-TiO2 1:1b) and zinc oxide (CS-ZnO 20:1a, CS-ZnO 20:1b) may be considered as a safe, non-cytotoxic packaging materials in the future.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Effect of Chitosan Films on Food Spoilage Bacteria

Wrońska

Katir

Miłowska

et al. 2021

IJMS

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“…Elevated morphological abnormalities and sperm motility are closely correlated related with this effect (53) . A study of Pinho et al (54) showed that exposing the testes of mice to different doses of ZnO NPs (1, 5, 10, and 20 µg) for 6 and 12 h led to changes in the cytoskeleton and nuclear structure in sperm cells, as well as an increase in the levels of reactive oxygen species (ROS) and DNA damage and thus cell death, indicating a toxic effect on sperm in a time and dose dependent.…”

Section: Discussionmentioning

confidence: 99%

Effect of Graphene Nanoparticles on some Organs of Reproductive System in Male Albino Mice

Asawir Saadi AliAL-kazomy,

Genan Adnan Al-Bairuty,

Mohammed Mahdi Jawad

2021

Indian Journal of Forensic Medicine & Toxicology

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Due to their exceptional physical properties, graphene nanomaterials have gained a great deal of interest in recent years for their application to state-of-the-art technology. On the other hand, nanotoxicity of graphene materials has also rapidly become a major concern, especially in the field of occupational health. There is a lack of evidence that such graphene nanomaterials influence the organs of the male reproductive system. Therefore, the goal of this study was to determine the effect of Graphene nanoparticles (GNPs) on mice body weight, reproductive organs weight and sperm quality, as well as certain reproductive organs injuries, after14 days of treatment with oral gavages of 0.1 ml of 10, 20, and 30 mg/kg of GNPs in male albino mice. The results showed a decrease in animal body weight and certain reproductive organs, as well as a decrease in the proportion of live sperm and the concentration of sperm in the tail of the epididymis and an increase in sperm abnormalities in all treatment relative to the control group. Injuries in the testes and epididymis (head and tail) were shown by histopathological analysis. This study concluded that oral GNPs gavages at various concentrations have a negative effect on the male reproductive system and can affect fertility.

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“…GO-AgNPs were sonicated for 20 min before use. Then, the GO-AgNP suspension was dispersed in DMEM/F12 at different concentrations (1,4,8,12, and 16 µg/mL) for 24 h at 37 • C. After culture, 10 µL of CCK-8 was added to each well and incubated for 30 min at 37 • C in the dark. The absorbance at 450 nm was measured using a microplate reader (BioTek Synergy 2, Winooski, VT, USA).…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…Nanotoxicity involves the understanding of the adverse biological effects of nanoparticles using both in vitro and in vivo model systems such as cells, tissues, organs, and organisms [ 1 ]. In vitro assays are the first approach to determining the cytotoxicity of nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide–Silver Nanoparticle Nanocomposites Induce Oxidative Stress and Aberrant Methylation in Caprine Fetal Fibroblast Cells

Yuan

Cai

Wang

et al. 2021

Cells

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Graphene oxide–silver nanoparticle (GO-AgNPs) nanocomposites have drawn much attention for their potential in biomedical uses. However, the potential toxicity of GO-AgNPs in animals and humans remains unknown, particularly in the developing fetus. Here, we reported the GO-AgNP-mediated cytotoxicity and epigenetic alteration status in caprine fetal fibroblast cells (CFFCs). In brief, the proliferation and apoptosis rate of GO-AgNP-treated CFFCs (4 and 8 µg/mL of GO-AgNPs) were measured using the cell-counting kit (CCK-8) assay and the annexin V/propidium iodide (PI) assay, respectively. In addition, the oxidative stress induced by GO-AgNPs and detailed mechanisms were studied by evaluating the generation of reactive oxygen species (ROS), superoxide dismutase (SOD), lactate dehydrogenase (LDH), malondialdehyde (MDA), and caspase-3 and abnormal methylation. The expression of pro- and anti-apoptotic genes and DNA methyltransferases was measured using reverse transcription followed by RT-qPCR. Our data indicated that GO-AgNPs cause cytotoxicity in a dose-dependent manner. GO-AgNPs induced significant cytotoxicity by the loss of cell viability, production of ROS, increasing leakage of LDH and level of MDA, increasing expression of pro-apoptotic genes, and decreasing expression of anti-apoptotic genes. GO-AgNPs incited DNA hypomethylation and the decreased expression of DNMT3A. Taken together, this study showed that GO-AgNPs increase the generation of ROS and cause apoptosis and DNA hypomethylation in CFFCs. Therefore, the potential applications of GO-AgNPs in biomedicine should be re-evaluated.

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In Vitro Cytotoxicity Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

Cited by 47 publications

References 81 publications

Antimicrobial Effect of Chitosan Films on Food Spoilage Bacteria

Antimicrobial Effect of Chitosan Films on Food Spoilage Bacteria

Effect of Graphene Nanoparticles on some Organs of Reproductive System in Male Albino Mice

Graphene Oxide–Silver Nanoparticle Nanocomposites Induce Oxidative Stress and Aberrant Methylation in Caprine Fetal Fibroblast Cells

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