In Vitro Cytotoxicity and Antibacterial Evaluation of Aqueous, Methanolic and Ethanolic Extracts of Anastatica hierochuntica against Pathogenic Bacteria

Abstract

A b s t r a c t A r t i c l e I n f oThe cytotoxic effect of Anastatica hierochuntica was evaluated in vitro using Vero cell lines, characterized by identification of cellular alterations microscopically and cellular viability colorimetrically. The mean CC 50 value of methanol, ethanol, and aqueous extract were lower than 1000 µg/ml. The antibacterial activity of ethanol, methanol and aqueous extracts of A. hierochuntica was evaluated using agar well diffusion method against 10 bacterial pathogens. All the extracts showed substantial antibacterial activity against the selected pathogens. The methanolic extract showed highest inhibition zone followed by the aqueous and ethanolic extract. Among the bacterial pathogens, L. monocytogenes was more resistant towards and ethanol extract. Although, other tested bacteria were susceptible towards aqueous ethanol and methanol extract. However, methanol extract was effective against the tested pathogens, when compared with the other extracts. The minimum inhibitory concentrations (MIC) ranged from 15-50 mg/ml to inhibit the growth of the tested bacteria. The results show that the plant contains bioactive components which can inhibit the bacterial pathogens which can be further purified and used against the multi-drug resistant bacteria.

Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells

“…Similarly, methanolic and ethyl acetate soluble fraction inhibited hepatotoxicity by 68.7% and 80.9% respectively, in a D-galactosamine-induced model ( Yoshikawa et al, 2003a ). AlSobeai (2016) demonstrated that the A. hierochuntica methanolic, ethanolic and aqueous extracts, tested at more than ≥1000 µg/ml, exerted no cytotoxic effects on a Vero cell line, providing evidence that it is only toxic to cancer cells. It was reasonable to study the antiproliferative activities of the aqueous and organic extracts of A. hierochuntica, considering the wide range of effective concentrations depending on the type of cancer cells and the duration of treatment.…”

Biological properties and identification of some active ingredients in Anastatica hierochuntica and Lepidium sativum, grown in Egypt

“…It is also important to note that the polar and non-polar phenolic compounds present in A. hierochuntica have been recently attributed to the nephroprotective, antioxidant and free radical scavenging activities [10]. The aqueous, ethanol and methanol extracts of A. hierochuntica have been shown to exhibit antibacterial activity against number of pathogenic bacteria which can lead to serious and life threatening complications such as sepsis, kidney and liver failure, toxic shock, and even death if it untreated [11].…”