In vitro Cytotoxic Properties of Crude Polar Extracts of Plants Sourced from Australia

Mani, Janice S.; Johnson, Joel B.; Hosking, Holly; Walsh, Kerry B.; Neilsen, Paul M.; Naiker, Mani

doi:10.1016/j.ccmp.2022.100022

Cited by 11 publications

(6 citation statements)

References 29 publications

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“…Several epidemiological studies revealed the beneficial effects of natural bioactive compounds, including alkaloids, polyphenols, terpenoids, or other phytochemicals, on cardiovascular, diabetes, obesity, neurological disorders, and cancers [ 31 , 32 , 33 , 34 ]. Australia has a wide range of native fruits and medicinal plants which have been used to treat different health disorders [ 35 ]. Kakadu plum, Davidson plum, quandong peach, and muntries have been used for several years in traditional medicine.…”

Section: Resultsmentioning

confidence: 99%

Phytochemicals, Antioxidant Activities, and Toxicological Screening of Native Australian Fruits Using Zebrafish Embryonic Model

Ali

Kiloni

Cáceres-Vélez

et al. 2022

Foods

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Phytochemicals play a pivotal role in human health and drug discovery. The safety evaluation of plant extracts is a prerequisite to ensure that all phytochemicals are safe before translational development and human exposure. As phytochemicals are natural, they are generally considered safe, although this is not always true. The objective of this study was to investigate and compare the phytochemical composition, antioxidant potential, and safety evaluation of native Australian Muntries (Kunzea pomifera), Kakadu plum (Terminalia ferdinandiana), Davidson plum (Davidsonia) and Quandong peach (Santalum acuminatum) through the in vivo vertebrate zebrafish embryonic model. The highest total phenolic content (TPC; 793.89 ± 22.27 μg GAE/mg) was quantified in Kakadu plum, while the lowest TPC (614.44 ± 31.80 μg GAE/mg) was quantified in Muntries. Developmental alterations, mortality, and morbidity were assessed for toxicological screening of these selected native Australian fruit extracts. In this study, muntries were quantified as having the least LC50 value (169 mg/L) compared to Davidson plum (376 mg/L), Kakadu plum (>480 mg/L), and Quandong peach (>480 mg/L), which indicates that muntries extract was more toxic than other fruit extracts. Importantly, we found that adverse effects were not correlated to the total phenolic content and antioxidant potential of these native Australian fruits and cannot simply be predicted from the in vitro analysis. Conclusively, these selected native Australian fruit extracts are categorized as safe. This study could explore the use of these native Australian fruits in cosmetics, pharmaceuticals, and drug discovery.

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Section: Resultsmentioning

confidence: 99%

Phytochemicals, Antioxidant Activities, and Toxicological Screening of Native Australian Fruits Using Zebrafish Embryonic Model

Ali

Kiloni

Cáceres-Vélez

et al. 2022

Foods

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“…A previous study has successfully demonstrated that two isolated flavonoid glycosides (2′ -hydroxyl neophellamuretin and 200-O-rhamnosylswertisin) isolated from Desmodium caudatum (Thunb.) DC had a certain growth inhibitory effect on HeLa cells in a dose dependent manner [ 25 ]. The chromatographic separation, isolation and characterization of glycosylated phenolic compound is often challenging due to their complex structural conformity arising through varying degree of plausible glycosylation patterns and high polarity [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Bioassay Guided Fractionation Protocol for Determining Novel Active Compounds in Selected Australian Flora

Mani

Johnson

Hosking

et al. 2022

Plants

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A large variety of unique and distinct flora of Australia have developed exceptional survival methods and phytochemicals and hence may provide a significant avenue for new drug discovery. This study proposes a bioassay guided fractionation protocol that maybe robust and efficient in screening plants with potential bioactive properties and isolating lead novel compounds. Hence, five native Australian plants were selected for this screening process, namely Pittosporum angustifolium (Gumbi gumbi), Terminalia ferdinandiana (Kakadu plum, seeds (KPS), and flesh (KPF)), Cupaniopsis anacardioides (Tuckeroo, seeds (TKS) and flesh (TKF)), Podocarpus elatus (Illawarra plum, seeds (IPS) and flesh (IPF)) and Pleiogynium timoriense (Burdekin plum, seeds (BPS) and flesh (BPF)). The methanolic extracts of the plants samples were analysed for Total phenolic content (TPC) and antioxidant capacity measure by FRAP. The highest values were found in the KPF which were 12,442 ± 1355 mg GAE/ 100 g TPC and 16,670 ± 2275 mg TXE/100 g antioxidant capacity. Extracts of GGL was deemed to be most potent with complete cell inhibition in HeLa and HT29, and about 95% inhibition in HuH7 cells. Comparative activity was also seen for KPS extract, where more than 80% cell inhibition occurred in all tested cell lines. Dose-dependent studies showed higher SI values (0.72–1.02) in KPS extracts than GGL (0.5–0.73). Microbial assays of the crude extracts were also performed against five bacterial strains commonly associated with causing food poisoning diseases were selected (Gram positive—Staphylococcus aureus and Gram negative—Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa bacteria). KPF extracts were effective in suppressing microbial growth of all tested bacterial strains except for P. aeruginosa, while TKS and TKF were only slightly effective against S. aureus. Due to the potential of the GGL crude extract to completely inhibit the cells compared to KPS, it was further fractionated and tested against the cell lines. HPLC phenolic profiling of the crude extracts were performed, and numerous peak overlaps were evident in the fruit extracts. The KPF extracts demonstrated the strongest peaks which was coherent with the fact that it had the highest TPC and antioxidant capacity values. A high occurrence of t-ferulic acid in the GGL extracts was found which may explain the cytotoxic activity of GGL extracts. Peaks in KPS and KPF extracts were tentatively identified as gallic acid, protocatechuic acid, 4-hydroxybenzoic acid and syringic acid and possibly ellagic acid. HPLC time-based fractionation of the GGL extract (F1–F5) was performed and Dose dependent cytotoxic effects were determined. It was construed that F1, having the highest SI value for HeLa, HT29 and HuH7 (1.60, 1.41 and 1.67, respectively) would be promising for further fractionation and isolation process.

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“…88 Some of these compounds are flavonoids, such as quercetin, kaempferol, and hesperidin, and polyphenols, such as coumaric acid and caffeic acid. 89…”

Section: Discussionmentioning

confidence: 99%

Anticancer Properties of Mexican Medicinal Plants: An Updated Review

Cuevas-Cianca,

González-Campos,

Mejía Méndez

et al. 2023

Natural Product Communications

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Mexico is considered a mega-diverse country due to its terrestrial, marine, and biological richness. Throughout history, Mexican medicinal plants have been used to elaborate decoctions, pastes, and powders to treat neoplastic, gastrointestinal, metabolic, neurodegenerative, skin, and infectious disorders. Cancer constitutes a group of diseases that result from the uncontrolled growth and proliferation of cells. Current treatment regimens against it encompass the administration of chemotherapy, radiotherapy, targeted therapy, and immunotherapy. Despite their possible efficacy, their use is often related to the possibility of relapse, the development of serious adverse events, toxic effects, and many drug-resistance mechanisms. As an alternative, Mexican medicinal plants have been extensively studied using their capacity to elicit strong anticancer activities and possess novel bioactive and safe compounds. This review concentrates on the knowledge gained in recent years (2011–2022) about the anticancer properties of extracts and isolated compounds from Mexican medicinal plants. Generalities, antioxidant activities, features of cancer cells, and drug-resistance mechanisms are reviewed in this work. In addition, the possible anticancer mechanisms of isolated compounds and the status of FDA-approved cancer drugs derived from plants are covered. Finally, our perspective on the future of traditional medicine and Mexican medicinal plants in cancer treatment is presented.

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In vitro Cytotoxic Properties of Crude Polar Extracts of Plants Sourced from Australia

Cited by 11 publications

References 29 publications

Phytochemicals, Antioxidant Activities, and Toxicological Screening of Native Australian Fruits Using Zebrafish Embryonic Model

Phytochemicals, Antioxidant Activities, and Toxicological Screening of Native Australian Fruits Using Zebrafish Embryonic Model

Bioassay Guided Fractionation Protocol for Determining Novel Active Compounds in Selected Australian Flora

Anticancer Properties of Mexican Medicinal Plants: An Updated Review

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