In vitro  cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Eljezi, Teuta; Pinta, P.; Richard, Damien; Pinguet, Jérémy; Chezal, Jean‐Michel; Chagnon, Marie‐Christine; Sautou, V.; Grimandi, G.; Moreau, Emmanuel

doi:10.1016/j.chemosphere.2017.01.026

Cited by 73 publications

(55 citation statements)

References 33 publications

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“…These cell cultures were maintained in 75 cm 2 flasks (Costar, Corning Inc., Corning, NY, USA) in standard culture conditions of 37 °C and 5% CO 2 , changing the media every 2 days. The cells were harvested by trypsinization using 0.05% Trypsin-EDTA solution (Gibco®) at 80–90% culture confluence and further sub cultivated into culture flasks 8 , 9 .…”

Section: Methodsmentioning

confidence: 99%

Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation

Srivastava

Alonso-Alonso

Fernandez-Bueno

et al. 2018

Sci Rep

124

112

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A series of recent acute blindness cases following non–complicated retinal detachment surgery caused the release of several health alerts in Spain. The blindness was attributed to certain lots of perfluoro-octane (PFO; a volatile and transient medical device). Similar cases have been reported in other countries. This has raised questions regarding the validity of cytotoxicity test methods currently used to certify the safety of PFO lots. The tests were performed according to the International Organization for Standardization (ISO) norms, using the extract dilution method or the indirect contact method as applied to L929 cells, a line derived from mouse fibroblasts. The limitations of those methods have been resolved in this study by proposing a new cytotoxicity test method for volatile substances. The new method requires direct contact of the tested substance with cells that are similar to those exposed to the substance in the clinical setting. This approach includes a few new technical steps that are crucial for detecting cytotoxicity. Our new method detected toxic PFO lots that corresponded to the lots producing clinical blindness, which previous methods failed to detect. The study suggests applying this new method to avoid occurrence of such cases of blindness.

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Section: Methodsmentioning

confidence: 99%

Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation

Srivastava

Alonso-Alonso

Fernandez-Bueno

et al. 2018

Sci Rep

124

112

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“…Additionally, in a study of DEHP and DEHP replacements utilizing a murine cell line, DEHP was more cytotoxic that DINCH, but the decrease in cell viability was ≤50% for both compounds. Interestingly, mono-(2-ethylhexyl) phthalate (MEHP, a DEHP metabolite) and several DINCH and DEHTP metabolites presented more cytotoxic dose-response effects than their parent compounds at the same concentrations, with MEHP having the smallest effect of all metabolites [41]. These findings highlight the need for characterizing and identifying sources of exposure to both phthalates and phthalate replacement chemicals, particularly among pregnant women and children.…”

Section: Introductionmentioning

confidence: 97%

Determinants and characterization of exposure to phthalates, DEHTP and DINCH among pregnant women in the PROTECT birth cohort in Puerto Rico

Rodríguez‐Carmona

Ashrap

Calafat

et al. 2019

J Expo Sci Environ Epidemiol

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Background As a result of evidence suggesting phthalate toxicity, their use has decreased in recent years. However, new phthalates and non-phthalate replacements have emerged in their place, with unknown potential impacts on health. Methods We measured 15 phthalate, two di(2-ethylhexyl)terephthalate (DEHTP), and two di(isononyl)cyclohexane-1,2dicarboxylate (DINCH) urinary metabolites, collected up to three times during pregnancy from 994 women in Northern Puerto Rico (2011-2017). We used tests of linear trend to assess changes in concentrations over time and linear mixed models to identify predictors of exposure (sociodemographic characteristics, drinking water sources, diet, product use). Results Several phthalate metabolites decreased over the study period indicating decreased exposure, while the geometric mean of DEHTP metabolites (molecular sum) increased threefold between 2014 and 2017. Intraclass correlations revealed low to moderate reproducibility of these biomarkers across pregnancy. Several metabolites were associated with maternal age, income, education, pre-pregnancy BMI, drinking public water, use of cleaning and personal care products, and ice cream consumption. DINCH metabolite concentrations remained low throughout the study period. Conclusion Although exposure to some phthalates may be decreasing, exposure to replacements, such as DEHTP, is increasing. Additional studies are needed to further characterize sources of phthalate replacement chemicals and potential exposure-related health effects among vulnerable populations.

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“…Due to the ongoing substitution process and considering new regulatory requirements, plasticizer substitutes have subsequently been added to the list of substances of interest. Several studies have been conducted to evaluate the migration behavior in poly(vinyl chloride) (PVC) medical devices that include these plasticizers of new generation [9] and to analyze their presence in different environments and in humans [10][11][12][13][14] in order to replace the use of DEHP. The ATBC incorporation has been also examined in other polymeric systems, like poly(lactic acid) and poly(hydroxybutyrate), among others [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Evaluation of the effect of DBP or DEHP on PP has been previously described in literature [7]. Nevertheless, unlike other polymers [10][11][12][13][14][15][16][17], there is, to our knowledge, no exhaustive study of the addition into PP of these safer plasticizers to simultaneously substitute the phthalate plasticizers and the PVC in this contact application with food or in that medical-clinical one. Therefore, the aim of this research is to proceed to the incorporation of a small amount of different less toxic plasticizers into an isotactic metallocene polypropylene (iPP) to evaluate its loss as a function of time by the action of thermo-oxidative treatment at 95 °C, and to comprehensively understand how degradation and plasticizer consumption affect the chemical details in the iPP, its crystalline characteristics, the features on surface of the film and the mechanical response.…”

Section: Introductionmentioning

confidence: 99%

Effect of thermo-oxidation on loss of plasticizers, on crystalline features and on properties in a metallocene isotactic polypropylene

Blázquez‐Blázquez

Barranco‐García

Cerrada

et al. 2019

Polymer

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Incorporation of a low content of several plasticizers into a metallocene isotactic polypropylene (iPP) has been performed in order to learn about their stability and performance by the action of a thermo-oxidative degradation treatment at 95 °C for different times. Comparison with the behavior exhibited by the material with di(2-ethylhexyl) phthalate (DEHP) is established for a feasible replacement of its use for contributing to environmental and human health preservation. Dependence of these aggressive degrading conditions on plasticizer loss is analyzed as well as elucidation of degradation influence on the iPP crystalline characteristics. The results show that chemical changes associated with this degradation process hinder formation of the orthorhombic γ crystalline lattice and postpone the overall crystallization capability. Accordingly, the final features in the film surface and the mechanical response are significantly affected by degradation time. The TOTM plasticizer can be considered an excellent candidate for DEHP substitution.

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In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Cited by 73 publications

References 33 publications

Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation

Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation

Determinants and characterization of exposure to phthalates, DEHTP and DINCH among pregnant women in the PROTECT birth cohort in Puerto Rico

Effect of thermo-oxidation on loss of plasticizers, on crystalline features and on properties in a metallocene isotactic polypropylene

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