In vitro cytotoxic activity of nine plants used in Mayan traditional medicine

Mena-Rejón, Gonzalo J.; Caamal-Fuentes, Edgar; Cantillo-Ciau, Zulema; Cedillo‐Rivera, Roberto; Flores-Guido, José Salvador; Moo-Puc, Rosa

doi:10.1016/j.jep.2008.11.012

Cited by 48 publications

(28 citation statements)

References 12 publications

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“…In this assay, mitochondrial dehydrogenase enzyme in metabolically active cells converts yellow tetrazolium salt to dark blue formazan and the intensity of blue colour predicts cell viability (Mena-Rejon et al 2009). The three extracts showed significant cytotoxicity against the investigated (KBM5, SCC4 and U266) cell lines, as represented in Figure 1 and Table 1.…”

Section: In Vitro Anticancer Activitymentioning

confidence: 99%

Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects ofPsidium guajavaleaf extracts

Ashraf

Sarfraz

Rashid

et al. 2016

Pharmaceutical Biology

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Context Psidium guajava L. (Myrtaceae) leaves are used in traditional medicines for the treatment of cancer, inflammation and other ailments. Objective The current study explores scientific validation for this traditional medication. Materials and methods We used ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl hydrazil (DPPH) assays to estimate antioxidant activity of P. guajava leaf extracts (methanol, hexane and chloroform). Antitumour and in vivo cytotoxic activities were determined using potato disc assay (PDA) and brine shrimp lethality assay, respectively. Three human carcinoma cell lines (KBM5, SCC4 and U266) were incubated with different doses (10-100 mg/mL) of extracts and the anticancer activity was estimated by MTT assay. NF-kB suppressing activity was determined using electrophoretic mobility shift assay (EMSA). Chemical composition of the three extracts was identified by GC-MS. Total phenolic and flavonoid contents were measured by colorimetric assays. Results and discussions The order of antioxidant activity of three extracts was methanol4chloro-form4hexane. The IC 50 values ranged from 22.73 to 51.65 mg/mL for KBM5; 22.82 to 70.25 mg/mL for SCC4 and 20.97 to 89.55 mg/mL for U266 cells. The hexane extract exhibited potent antitumour (IC 50 value ¼ 65.02 mg/mL) and cytotoxic (LC 50 value ¼ 32.18 mg/mL) activities. This extract also completely inhibited the TNF-a induced NF-kB activation in KBM5 cells. GC-MS results showed that pyrogallol, palmitic acid and vitamin E were the major components of methanol, chloroform and hexane extracts. We observed significant (p50.05) difference in total phenolic and flavonoid contents of different solvent extracts. Conclusion The present study demonstrates that P. guajava leaf extracts play a substantial role against cancer and down-modulate inflammatory nuclear factor kB. ARTICLE HISTORY

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Section: In Vitro Anticancer Activitymentioning

confidence: 99%

Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects ofPsidium guajavaleaf extracts

Ashraf

Sarfraz

Rashid

et al. 2016

Pharmaceutical Biology

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“…The results were statistically analyzed with GraphPad Prism 4 software. The selectivity index (SI) of the extract was defined as the ratio of cytotoxicity on Vero cells to cancerous cells (Mena-Rejon et al, 2008).…”

Section: Cytotoxicity Of Extracts Using Xtt Kitmentioning

confidence: 99%

In vitro chemo-preventative activity of Crotalaria agatiflora subspecies agatiflora Schweinf

Roux

Hussein

Lall

2011

Journal of Ethnopharmacology

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Ethnopharmacological relevance: Crotalaria species have been widely used in Chinese traditional medicine to treat several types of internal cancers. Crotalaria agatiflora is used as a medicinal plant in several African countries for the treatment of bacterial and viral infections as well as for cancer. Materials and methods:Water and ethanol extracts of the leaves of C. agatiflora were evaluated for cytotoxcity on four cancerous and one noncancerous cell lines, using XTT (Sodium 3' -[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro) benzene sulfonic acid hydrate) colorimetric assay. Antioxidant activity was determined using DPPH (1,1-Diphenyl-2-picryl hydrazyl). Light microscopy (eosin and hematozylin staining) and flow cytometry (Annexin-V and propidium iodide) were used to evaluate the mechanism of action of the ethanol extract and one of the isolated compounds. Results Conclusions:The results indicated that C. agatiflora possesses potential chemopreventative and therapeutic properties. The exact mechanism of action should still be determined in future studies.It is hypothesised that the ethanolic extract as well as madurensine induces autophagy, which in prolonged circumstances may lead to autophagic cell death.

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“…In addition, studies of the toxicological effects of plant extracts and their isolated compounds on normal cells should be included. Canine kidney (MDCK), mouse fibroblasts (L929), Rhesus monkey kidney from Macaca mullata cells (LLC-MK2) and human peripheral blood mononuclear cells (Sanchez et al, 2001;FrancoMolina et al, 2003;Mena-Rejon et al, 2009) have been used as normal cells in cytotoxic studies.…”

Section: Introductionmentioning

confidence: 99%

Mexican medicinal plants used for cancer treatment: Pharmacological, phytochemical and ethnobotanical studies

Alonso-Castro

Villarreal

Salazar‐Olivo

et al. 2011

Journal of Ethnopharmacology

254

127

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In vitro cytotoxic activity of nine plants used in Mayan traditional medicine

Cited by 48 publications

References 12 publications

Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects ofPsidium guajavaleaf extracts

Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects ofPsidium guajavaleaf extracts

In vitro chemo-preventative activity of Crotalaria agatiflora subspecies agatiflora Schweinf

Mexican medicinal plants used for cancer treatment: Pharmacological, phytochemical and ethnobotanical studies

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