In-vitro cytocompatibility of dental resin monomers on osteoblast-like cells

Kraus, Dominik; Wolfgarten, Markus; Enkling, Norbert; Helfgen, Ernst-Heinrich; Frentzen, Matthias; Probstmeier, Rainer; Winter, Jochen; Stark, Helmut

doi:10.1016/j.jdent.2017.07.008

Cited by 39 publications

(41 citation statements)

References 36 publications

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“…40 On the other hand, PA causes less apoptotic induction than other SARCs. Kraus et al 41 detected that cytotoxic effects of the monomers were ranked as Bis-GMA, UDMA, TEGDMA, and HEMA from high to low after 24-and 72 h exposure periods. 42 Alkurt et al 20 examined the cytotoxic effects of PA on pulp and gingival cells for 24-and 48 h exposures and found greater cytotoxicity after 48 h compared to 24 h. They also showed that the toxicity of PA depends on the extract concentration.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

et al. 2020

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PURPOSE. The effects of four different self-adhesive resin cement materials on cell viability and apoptosis after direct and indirect exposure were evaluated using different cell culture techniques. MATERIALS AND METHODS. Self-adhesive cements were applied to NIH/3T3 mouse fibroblasts by the extract test method, cell culture inserts, and dentin barrier test method. After exposure periods of 24 h and 72 h, the cytotoxicity of these self-adhesive materials was evaluated using the MTT assay (viability) and the Annexin-V-FITC/PI staining (apoptosis). RESULTS. The lowest cell viability was found in cells exposed to BeautiCem SA for 24 h in the extract test method. Cell viability was reduced to 70.6% compared to negative controls. After the 72 h exposure period, viability rate of cell cultures exposed to BeautiCem SA decreased more than 2-fold (29.5%) while cells exposed to RelyX U200 showed the highest viability rate of 71.4%. In the dentin barrier test method, BeautiCem SA induced the highest number of cells in apoptosis after a 24 h exposure (4.1%). Panavia SA Cement Plus was the material that caused the lowest number of cells in apoptosis (1.5%). CONCLUSION. The used self-adhesive cements have showed different cytotoxic effects based on the evaluation method. As exposure time increased, the materials showed more cytotoxic and apoptotic effects. BeautiCem SA caused significantly more severe cytotoxic and apoptotic effects than other cements tested. Moreover, cements other than BeautiCem SA have caused necrotic cell death rather than apoptotic cell death.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

et al. 2020

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“…It has been well documented that resin cement, which is usually used for luting, may be toxic to the tissues. Kraus et al have showed that monomers from resin cement can be detrimental to osteoblast-like cells (Kraus et al, 2017). Several clinical studies, like Novoa et al, detected statistically significant more bone loss around 1-mm-height multiunit abutment, compared to 2.5 mm (Nóvoa et al, 2017) or 3.0 mm heights (Blanco et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Influence of titanium base, lithium disilicate restoration and vertical soft tissue thickness on bone stability around triangular‐shaped implants: A prospective clinical trial

Linkevičius

Alkimavičius

et al. 2018

Clinical Oral Implants Res

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“…The exposure time was 45 min, following the manufacturer's instructions for clinical application. Administration vehicles were used as controls, sterile water in G1 and G4 and dimethylsulfoxide (DMSO) in G2 and G3 groups were used in the same volume for HP and Zoom ® administration [18].…”

Section: Cell Treatmentmentioning

confidence: 99%

“…Cell viability was evaluated through the annexin-V/propidium iodide (AV/PI) incorporation assay, which allows a distinction between live, early apoptotic, late apoptotic/necrotic, and necrotic cells [18]. Briefly, 10 6 cells were incubated for 15 min in 100 µL of binding buffer, 1 µL of AV-FICT, and 5 µL of IP (KIT Immunotech, Marseille, France), during 15 min at room temperature and in the dark, according to the kit manufacturer's instructions, followed by flow cytometry analysis [19].…”

Section: Cell Viabilitymentioning

confidence: 99%

“…Production of intracellular peroxides was performed with non-fluorescent probe 2,7-dichlorodihydrofluorescein diacetate (DCFH2-DA). This liposoluble compound enters cells and accumulates at cytosol and is cleaved by intracellular esterases in 2,7-dichlorodihydrofluorescein (DCFH2), which in the presence of peroxides is oxidated to dichlorodihydrofluorescein (DCF), a green fluorescent compound easily visualized with excitation and emission wavelengths of 504 nm and 529 nm, respectively [18]. Emitted fluorescence and intracellular peroxide concentrations are proportional [20].…”

Section: Detection Of Intracellular Peroxidesmentioning

confidence: 99%

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Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

et al. 2020

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Tooth whitening procedures are increasing; however, side effects can occur, such as damage to pulp cells, by the whitening products. This study aims to assess the cellular effects promoted by a whitening product, namely, the oxidative stress fostered by the active agent hydrogen peroxide, with and without photoactivation. Additionally, if cellular recovery occurred, we intended to determine the time point where cells recover from the tooth whitening induced damage. Human fibroblasts were exposed to hydrogen peroxide, Zoom®, Zoom® + irradiation, and irradiation alone. The following analysis was performed: metabolic activity evaluation by the MTT assay; cell viability, mitochondrial membrane potential, peroxides production, superoxide radical production, and reduced glutathione expression by flow cytometry. We determined the IC50 value for all groups, and a dose-dependent cytotoxic effect was verified. At the times analyzed, hydrogen peroxide groups showed no metabolic activity recovery while a cell recovery was observed after 24 h (Zoom®) and 48 h (Zoom® + irradiation). Cell death was seen in hydrogen peroxide and Zoom® + irradiation groups, mainly by apoptosis, and the irradiation had a cytotoxic effect per se. This in vitro study supports that whitening products with moderate hydrogen peroxide (HP) concentration have a temporary effect on cells, allowing a cellular recovery.

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In-vitro cytocompatibility of dental resin monomers on osteoblast-like cells

Cited by 39 publications

References 36 publications

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

Influence of titanium base, lithium disilicate restoration and vertical soft tissue thickness on bone stability around triangular‐shaped implants: A prospective clinical trial

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

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