In vitro culture technique for Cryptocaryon irritans, a parasitic ciliate of marine teleosts

Elicited cross-protection and specific antibodies in Mozambique tilapia (Oreochromis mossambicus) against two different immobilization serotypes of Cryptocaryon irritans isolated in Hawaii

“…One hundred protomonts were collected and washed four times and placed in sterile 120 ml specimen collection containers (Starplex Scientific, Ontario, Canada) with filtered (0.2 mm) seawater and supplemented with 100 I.U. ml À1 Penicillin G potassium and 100 mg/ml streptomycin sulphate [24]. After confirmation of encystment of the tomont, cultures were incubated for 2e3 weeks at 22e23 C. Newly hatched theronts from the culture were used for DNA extraction, immobilization assay, and future propagation, and the remaining theronts were stored in sterile phosphate-buffered saline (PBS) at À80 C until processing.…”

Immobilization antigen vaccine adjuvanted by parasitic heat shock protein 70C confers high protection in fish against cryptocaryonosis

“…Apart from B cells and T H cells, cytotoxic T cell responses to C. irritans infections can also be important in vaccination-induced resistance [40,10]. The amount of mucus iAg-specific IgM antibody detected by ELISA correlated positively with the immobilization activity, but the level of protection was higher in iAg:Hsp70C group (Fig.…”

Immune protection of Mozambique tilapia (Oreochromis mossambicus) exposed to different infectious doses of ectoparasite (Cryptocaryon irritans)

“…One hundred protomonts were collected and washed four times and placed in a well of 24-well culture plate with filtered (0.2 μm pore-size filter) seawater and supplemented with 100 I.U. per ml penicillin G potassium and 100 μg per ml streptomycin sulfate (Yoshinaga et al 2007). After confirmation of encystment of the tomonts, cultures were incubated at room temperature.…”