In vitro culture of tomato ovaries

Kano, Kunio

doi:10.2503/jjshs.31.198

Cited by 4 publications

(2 citation statements)

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“…Therefore, in this study, we established a rice spikelet culture system as a tool for investigating rice fruit development. The ovary culture technique has been applied in a wide variety of plant species, such as Cucumis anguria, Solanum lycopersicum, wheat, Iberis amara, Althaea rosea, Zephyranthes sp., Dendrobium nobile and maize (Nitsch 1951;Rédei and Rédei 1955;Maheshwari and Lal 1961;Chopra 1962;Kano 1962;Sacher and Guha 1962;Ito 1966;Higgins and Petolino 1988;Mathias and Boyd 1988). In our culture system, when pollinated aF-spikelets were cultured on hormone-free medium, the ovary, in which an embryo and endosperm were formed, developed into a fruit.…”

Section: Discussionmentioning

confidence: 99%

Rice fruit development is associated with an increased IAA content in pollinated ovaries

Uchiumi

Okamoto

2010

Planta

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To analyze fruit set and early fruit (caryopsis) development in rice, we established an in vitro spikelet culture system. The ovary of cultured pollinated spikelets grew rapidly and developed into fruits with an embryo and endosperm. When unpollinated spikelets were cultured on a medium containing 2,4-dichlorophenoxyacetic acid, parthenocarpic fruits lacking an embryo and endosperm developed. The number and size of the cells in the pericarp of parthenocarpic fruits were almost identical to those of fruits induced by pollination, and degeneration of nucellus tissue was observed in both pollinated and parthenocarpic fruits. These results suggested that ovary growth was induced through increased auxin content in the spikelets. Quantitative measurement of indole-3-acetic acid (IAA) content in the spikelets indicated that the IAA level increased after pollination. Further analysis of IAA contents in the ovary and rachilla-pedicel of cultured spikelets suggested that fruit development is associated with IAA synthesis in the ovary following pollination/fertilization and subsequent transport of IAA from the ovary to the rachilla-pedicel. Partial or complete removal of the rachilla and/or pedicel prior to spikelet culture greatly inhibited fruit development. These results indicated that the rachilla and pedicel are essential for rice fruit development. AUX/IAA and ARF genes that might be involved in rice fruit development were identified through transcriptome analysis.

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Section: Discussionmentioning

confidence: 99%

Rice fruit development is associated with an increased IAA content in pollinated ovaries

Uchiumi

Okamoto

2010

Planta

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“…In vitro culture is one of available means in reducing and eliminating of these influences on the ovary development. Although in vitro culture of excised ovary has succeeded in some species (Kano, 1962;Nitsch, 1951;Wittenbach and Bukovac, 1980) IAA had a slight stimulating effect (Figs. 2 and 3).…”

Section: Phytohormonesmentioning

confidence: 99%

Synergism of Gibberellin and Auxin in 'Delaware' Grape Ovary Development in Vitro

Shiozaki

Ueda

Ogata

et al. 1995

Engei Gakkai zasshi

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SummaryThe correlative effect of gibberellin A3 (GA3) and indole-3-acetic acid (IAA) on the development of 'Delaware' grape ovary was examined in vitro.GA3 induced a significant increase of the ovary fresh weight, proportionate to its concentration; IAA and tryptophan (Trp), a precursor of IAA, had little effect on increasing in fresh weight of the ovary. GA3 + IAA increased the ovary fresh weight more than GA3 or IAA alone. The fresh weights of the GA3 or IAA treated ovaries increased 1.08-to 1.56-fold over the control, whereas those treated with the combination of GA3 and IAA increased 1.85-to 2-fold over control after a 9-10 week-culture. The effect of GA3+ Trp on increasing in fresh weight of the ovary was about the same as that of GA3+IAA. These results indicate that GA3 promotes the ovary development more than IAA and that IAA and Trp enlarge the promotive effect of GA3.The ovary development in response to GA3 and GA3 + Trp was partly inhibited by anti-auxins such as N-(1-naphthyl) phthalamic acid (NPA), 2,3,5-triiodobenzoic acid (TIBA). Besides inducing an increase of the ovary fresh weight, GA3 increased the content of IAA-like substances in the ovary. From these results, it appears that the effect of GA3 on the ovary development is ascribed not only to its original action, but also to the synergistic action with IAA synthesized de novo.

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Culture of Ovaries

Rangan¹

1984

Laboratory Procedures and Their Applications

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In vitro culture of tomato ovaries

Cited by 4 publications

References 30 publications

Rice fruit development is associated with an increased IAA content in pollinated ovaries

Rice fruit development is associated with an increased IAA content in pollinated ovaries

Synergism of Gibberellin and Auxin in 'Delaware' Grape Ovary Development in Vitro

Culture of Ovaries

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