In vitro culture of the seagrass Halophila decipiens

Bird, Kimon T.; Johnson, Judith L.; Jewett-Smith, Jerilyn

doi:10.1016/s0304-3770(97)00093-4

Cited by 32 publications

(26 citation statements)

References 10 publications

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“…However, elevated levels of inorganic nitrogen in the water layer may also be important. These compounds have been shown to cause severe toxicity in seagrasses and may therefore help explain these dramatic events (Burkholder et al 1992;van Katwijk et al 1997;Bird et al 1998;Brun et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Toxicity of reduced nitrogen in eelgrass (Zostera marina) is highly dependent on shoot density and pH

et al. 2008

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In sheltered, eutrophicated estuaries, reduced nitrogen (NH x ), and pH levels in the water layer can be greatly enhanced. In laboratory experiments, we studied the interactive eVects of NH x , pH, and shoot density on the physiology and survival of eelgrass (Zostera marina). We tested long-term tolerance to NH x at pH 8 in a 5-week experiment. Short-term tolerance was tested for two shoot densities at both pH 8 and 9 in a 5-day experiment. At pH 8, eelgrass accumulated nitrogen as free amino acids when exposed to high loads of NH x , but showed no signs of necrosis. Low shoot density treatments became necrotic within days when exposed to NH x at pH 9. Increased NH 3 intrusion and carbon limitation seemed to be the cause of this, as intracellular NH x could no longer be assimilated.Remarkably, experiments with high shoot densities at pH 9 showed hardly any necrosis, as the plants seemed to be able to alleviate the toxic eVects of high NH x loads through joint NH x uptake. Our results suggest that NH x toxicity can be important in worldwide observed seagrass mass mortalities. We argue that the mitigating eVect of high seagrass biomass on NH x toxicity is a positive feedback mechanism, potentially leading to alternative stable states in Weld conditions.

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Section: Introductionmentioning

confidence: 99%

Toxicity of reduced nitrogen in eelgrass (Zostera marina) is highly dependent on shoot density and pH

et al. 2008

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“…There are 2 reasons to re-assess the current view on nutrient cycling in seagrass meadows. Firstly, most studies on nutrient uptake by seagrass plants focus solely on inorganic nutrients and very little is known about uptake of organic nutrients (McRoy & Goering 1974, Bird et al 1998. Consequently, we lack insight into the role of (1) dissolved organic nutrients that are usually more abundant than dissolved inorganic nutrients in oligotrophic environments and (2) nutrient recycling within seagrass meadows via microbial decomposition of POM in the water column and/or in the sediment.…”

Section: Introductionmentioning

confidence: 99%

Nutrient dynamics of seagrass ecosystems: 15N evidence for the importance of particulate organic matter and root systems

Evrard

Kiswara

Bouma

et al. 2005

Mar. Ecol. Prog. Ser.

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“…The effect of plant growth regulators has been previously reported in seagrasses, such as P. oceanica and R. maritima, in which cytokinin induced shoot and rhizome growth (Loquès et al, 1990;Koch and Durako, 1991) whilst auxin increased shoot growth and branch production in rhizome segments obtained from seeds in Halophila decipiens (Bird et al, 1998). Recently, auxin naftal and IBA were reported to increase root emergence and growth in seedlings of P. oceanica (Balestri and Bertini, 2003).…”

Section: Discussionmentioning

confidence: 87%

“…In vitro, it has not been so extensively studied but it should be noted that Bird et al (1998) reported that H. decipiens in stage II of propagation progressed further to the acclimatisation stage, since it spontaneously rooted. Perhaps, it progressed spontaneously precisely because it rooted.…”

Section: Discussionmentioning

confidence: 99%

“…Whilst it is clear that it is possible to apply the essentials of in vitro methodology to establish explant cultures (i.e. stage I), massive shoot induction, rooting control and plant production (stages II and III) aquatic plants, such as the above mentioned species, have not been achieved (Loquès et al, 1990;Koch and Durako, 1991;Terrados-Muñoz, 1995;Bird et al, 1993Bird et al, , 1996Bird et al, , 1998.…”

Section: Introductionmentioning

confidence: 99%

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Anatomical and nutritional requirements for induction and sustained growth in vitro of Cymodocea nodosa (Ucria) Ascherson

García‐Jiménez¹,

Navarro²,

Santana³

et al. 2006

Aquatic Botany

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In vitro methods of plant micro-propagation are being considered as a possible solution for the decline in seagrass communities registered worldwide. To achieve successful plant micro-propagation, culture conditions are commonly adjusted empirically within almost species-specific conditions, to comply to the following three conditions: (i) culture establishment (ii) shoot production and (iii) rooting and hardening for planting in soil. Cultures of Cymodocea nodosa were established from axenic explants of the apical meristem (approx. 0.5 cm) which regenerated new leaf or produced leaf regenerating calli (5% of cultivated explants) in media containing 10 À6 M of the cytokinin analogue TDZ. Longer ramet explants, not fully axenic, containing internode with leaf and roots were also affected by 10 À6 M cytokinins and auxin type of regulators, as they promoted leaf extension (in cm), particularly GA. None of the explants progressed further to massive shoot propagation and new plantlet production. Instead, experiments made with ramet explants which simulated potential produced plantlet revealed that there seems to be a strong interaction within leaf, rhizome and root, since the carbon fixed in the leaf was rapidly translocated to the rest of the tissue (50% in the roots in a FW basis). The explants preferred ammonium and dihydrogen inorganic phosphate as a nutrient source, efficiently assimilating the former regardless of whether such were added to the underground or surface tissue. However, underground tissue was required to maintain P status in the cultivated explants. #

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In vitro culture of the seagrass Halophila decipiens

Cited by 32 publications

References 10 publications

Toxicity of reduced nitrogen in eelgrass (Zostera marina) is highly dependent on shoot density and pH

Toxicity of reduced nitrogen in eelgrass (Zostera marina) is highly dependent on shoot density and pH

Nutrient dynamics of seagrass ecosystems: 15N evidence for the importance of particulate organic matter and root systems

Anatomical and nutritional requirements for induction and sustained growth in vitro of Cymodocea nodosa (Ucria) Ascherson

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