In Vitro Culture of ‘Myrobalan’ (Prunus cerasifera Ehrh.) Embryos

Arbeloa, A.; Daorden, M. E.; Garcia, E.; Andreu, P.; Marín, Juan Antonio Antolinos

doi:10.21273/hortsci.44.6.1672

Cited by 17 publications

(7 citation statements)

References 23 publications

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“…Sources indicate that there are likely germination inhibitors present in the cotyledon and testa in Prunus and simply removing testa increased in vitro seed germination of peach by 10% (S xan and Yildirim, 2009). Arbeloa et al (2009) reported 60% higher germination of Prunus cerasifera 'Myrobalan' embryos 6.5-10 mm long compared with embryos that were 0.5-2 mm, which indicates that regardless of our seed treatment the germination likely would have remained modest in the 6 and 9 WAP collections. Although shoot emergence at 12 WAP with cold stratification was higher than the groups in Expt.…”

Section: Resultsmentioning

confidence: 90%

In Vitro Germination of Immature Prunus lusitanica Seed

Schulze

Lattier

Contreras

2017

horts

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A tissue culture protocol was developed to germinate immature Prunus lusitanica seeds in vitro. The study was conducted by first identifying the best media for germination, followed by investigating effects of seed conditioning. In Expt. I, seeds were collected 12 weeks after pollination (WAP) ± 1 week and placed on media after removing the pericarp. Eight different MS media (Murashige and Skoog, 1962) were tested (M1–M8) containing two concentrations each of 6-benzylaminopurine (BA), gibberellic acid (GA3), and sucrose. The longest shoots resulted from M4 (1.45 µm GA3, 6 µm BA, and 30 g·L−1 sucrose), followed by M1 (0 µm GA3, 3 µm BA, and 30 g·L−1 sucrose). Radicle and shoot emergence was greater than or equal to 90% for M1, M3, and M4 after a stratification treatment. In Expt. II, M1 was used to test root and shoot emergence at 6, 9, and 12 WAP, with and without cold stratification. Little success was seen 6 and 9 WAP, with only callus development in 6 WAP, nonstratified seed. Cold stratification increased shoot emergence in the 12 WAP group from 4% to 28%, appearing to be critical for shoot emergence. If the cotyledons are retained on the seed, future efforts to expedite breeding of P. lusitanica using in vitro germination should not be collected before 12 WAP and will benefit from cold stratification before germinating on M1 or M4. Chemical names: 6-benzylaminopurine (BA), gibberellic acid (GA3).

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Section: Resultsmentioning

confidence: 90%

In Vitro Germination of Immature Prunus lusitanica Seed

Schulze

Lattier

Contreras

2017

horts

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“…However, seeds of temperate fruit species require a long time to germinate under traditional stratification or natural conditions. Therefore, fast and uniform germination techniques are desirable for fruit-growing and breeding studies [14]. Various methods have been tried to overcome dormancy of stone fruits.…”

Section: Introductionmentioning

confidence: 99%

The Control of Apricot Seed Dormancy and Germination by Low Temperature Treatments

El-Yazal

Ei-Yazal

2021

Innovare J Ag Sci

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Objective: Freshly harvested seeds of “Local” apricot variety were found to be dormant and did not germinate at all. A specific low-temperature stratification treatment was required to overcome seed dormancy. The most effective temperature for breaking seed dormancy, germination, and seedling growth was 5°C cold stratification (CS). Increased seed germination percentage was recorded when the period of stratification prolonged. Seedling developed from stratified seeds had better growth than those developed from non-stratified seeds. Methods: For stratification treatments, the seeds with removed endocarp were mixed with moistened sand. Afterward, they were subjected to a period of stratification at 5°C. Seeds were stratified in pots of 30 cm×40 cm. Stratified seeds were regularly irrigated once per week. To prevent the water loss during stratification upper surface of pots was covered by a sack. The following stratification was applied for apricot variety: CS for 0, 3, 6, 9, 12, and15 days in 1998 and 1999 years for “Local” variety. Results: Apricot seeds required a CS of about 15 days for “Local” variety to reach maximum germination and normal seedling growth. Moreover, when stratification period was prolonged, some of the chemical constituents of apricot seeds were increased and other was decreased. Therefore, it can be suggested that breaking of dormancy is coincided with several changes in different chemical constituents of seeds. Some of these materials increased (total, reducing and non-reducing sugars, total free amino acids, total indoles, and total and conjugated phenols) and other materials such as free phenols which decreased at seed germinations. Conclusion: The most effective temperature for breaking seed dormancy, germination, and seedling growth was 5°C CS. Increased seed germination percentage was recorded when the period of stratification prolonged. Seedling developed from stratified seeds had better growth than those developed from non-stratified seeds.

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“…The diverse concentrations of plant growth regulators (PGRs) and mineral elements affect the in vitro propagation of clonal rootstock. There are limited in vitro studies on the micropropagation of Myrobalan 29C rootstocks (Arbeloa et al, 2009;Akşehirli, 2010;Movsiuw, 2011;Plopa et al, 2012;Özbek et al, 2014;Shabani et al, 2015;Nasri et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro micropropagation protocol for Myrobalan 29C rootstock

Güney¹

2019

Turk J Agric For

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Stone fruits are well known for their high nutritional value. Therefore, in horticulture, the micropropagation of suitable rootstocks is vital for their cultivation. The aim of the present study was to improve the micropropagation protocol of Myrobalan 29C (Prunus cerasifera Ehrh.) rootstock using shoot-tip culture. Murashige and Skoog (MS) basal medium containing 2 mg L-1 6-benzylaminopurine (BAP) and 0.05 mg L-1 gibberellic acid (GA 3) resulted in the highest number (14.3) of multiple shoots. However, a greater shoot length (2.0 cm) was attained when GA 3 was excluded from the MS medium and the concentration of BAP was reduced to 1 mg L-1. Root induction was best in ½MS medium containing 0.5 mg L-1 indole-3-butyric acid (IBA), 0.5 mg L-1 α-naphthaleneacetic acid, and 10 mL L-1 (≈13 mg L-1 Fe) ethylenediamine di-2-hydroxyphenyl acetate ferric with 7.0 roots per explant. On the other hand, the longest root (12.5 cm) was obtained from increased concentration to 1 mg L-1 of IBA. The establishment of a well-defined micropropagation protocol will lead to further biotechnological improvement of this crop.

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In Vitro Culture of ‘Myrobalan’ (Prunus cerasifera Ehrh.) Embryos

Cited by 17 publications

References 23 publications

In Vitro Germination of Immature Prunus lusitanica Seed

In Vitro Germination of Immature Prunus lusitanica Seed

The Control of Apricot Seed Dormancy and Germination by Low Temperature Treatments

Development of an in vitro micropropagation protocol for Myrobalan 29C rootstock

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