In Vitro Culture of Floral Buds of Aquilegia

Tepfer, Sanford S.; Greyson, R. I.; Craig, William R.; Hindman, Joseph L.

doi:10.1002/j.1537-2197.1963.tb06587.x

Cited by 36 publications

(4 citation statements)

References 12 publications

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“…Many investigators have cultured post-anthesis flowers for the study of fruit physiology (Nitsch, 1965). It is only in recent years that immature flower buds have been grown to gain an insight into the factors controlling floral morphogenesis (Galun, Jung, andLang, 1962, 1963;Tepfer, Greyson, Craig, and Hindman, 1963;Tepfer, Karpoff, and Greyson, 1966;Blake, 1966;Porath and Galun, 1967;Brulfert and Fontaine, 1967). There are only two reports of the occurrence of microsporogenesis in cultured flower buds (Blake, 1966;Porath and Galun, 1967).…”

Section: Introductionmentioning

confidence: 99%

Morphogenic Potentialities of Flower Buds of Kalanchoe pinnata Pers. grown in vitro

Ram

Wadhi

1968

Annals of Botany

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Excised flower buds of Kalanchoe pitmata Pers. representing two early stages of development (designated sets I and II), were cultured on modified White's medium (WB). They failed to attain full development on WB or on WB containing any of the following supplements: indole-3yl-acetic acid (IAA), 2,4dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), kinetin, or coconut milk (CM). A slight stimulation of the growth of corolla was caused by kinetin (10 ppm). IAA (1 ppm) and NAA (1 ppm) induced rooting from the cut end of the pedicel and from the proliferated torus tissue situated between the sepals and petals. 2,4-D (1 ppm) either singly or in concert with CM (10 per cent) stimulated the formation of shoot buds and root growth. Addition of kinetin (1 and 10 ppm) to WB favoured shoot formation, but suppressed rooting. Flower buds of set II developed shoot buds more readily than those of set I. Thus, the primordia of floral organs present in the immature buds lose their ability for normal morphogenesis under culture conditions. Buds destined to form flowers can be made to revert to vegetative growth.

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Section: Introductionmentioning

confidence: 99%

Morphogenic Potentialities of Flower Buds of Kalanchoe pinnata Pers. grown in vitro

Ram

Wadhi

1968

Annals of Botany

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“…In C. ajacis , the delayed growth of the six reduced petals is more evident than that of the confluent petal, while the reduced petals disappear at anthesis. The delayed development of the petals and even the stamens on the outer whorl (i.e., the second series) has been widely documented in Ranunculaceae [4,8,13,34,46,53,54], the core eudicots [55], and even in other angiosperms [56]. Furthermore, the temporal and spatial correlations in delayed development, as seen in this study, within the second series have also been reported previously [5].…”

Section: Discussionmentioning

confidence: 99%

Floral Organogenesis in Three Members of the Tribe Delphinieae (Ranunculaceae)

Chang

Downie

Peng

et al. 2019

Plants

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Three species (Aconitum taipeicum, Delphinium giraldii, and Consolida ajacis) of the tribe Delphinieae (Ranunculaceae) were examined using scanning electron microscopy and histological methods. The results showed that members of Delphinieae differ from their polysymmetrical relatives by four unique features: (1) a spiral phyllotaxis of their perianth and stamens, and a series of carpels, which initiated superficially in a whorl-liked arrangement; (2) sepal 2 being the largest one among the five sepals and becoming helmet-shaped or having a spur; (3) petals 2 and 5 initiated adaxially of sepal 2 and also becoming spurred; and (4) the monosymmetry of the first flower becoming established when sepal 2 becomes the largest. Major differences among the species include the timing of development of the second series; the fusion of two petals into a single one in C. ajacis; and, during early developmental stages, the two young spurred petals giving rise to a stalk and two bulges in A. taipeicum, a single bulge in D. giraldii, or an arch blade in C. ajacis. The unequal growth of the perianth, together with the reduction and the rearrangement of the carpels, are critical in inducing the symmetrical transformation of the flowers.

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“…During early oral meristem development in Aquilegia, anthers appear before laments [10,42]. Thus, the genetic pathways affecting A. jonesii'sretention of anthers in the staminode whorls could be at least partially epistatic to processes affecting lament morphology in the LE and FU subtraits.…”

Section: Fading Boundary Between Stamens and Staminodes In A Jonesiimentioning

confidence: 99%

Loss of staminodes in Aquilegia jonesii reveals a fading stamen-staminode boundary

Johns,

Min,

Ballerini

et al. 2024

Preprint

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The modification of fertile stamens into sterile staminodes has occurred independently many times in the flowering plant lineage. In the genus Aquilegia (columbine) and its closest relatives, the two stamen whorls closest to the carpels have been converted to staminodes. In Aquilegia, the only genetic analyses of staminode development have been reverse genetic approaches revealing that B-class floral identity genes are involved. A. jonesii, the only species of columbine where staminodes have reverted to fertile stamens, allows us to explore the genetic architecture of staminode development using a forward genetic approach. We performed QTL analysis using an outcrossed F2 population between A. jonesii and a horticultural variety that makes fully developed staminodes, A. coerulea ‘Origami’. Our results reveal a polygenic basis for staminode loss where the two staminode whorls are under some level of independent control. We also discovered that staminode loss in A. jonesii is not complete, in which staminode-like traits sometimes occur in the inner fertile stamens, potentially representing a fading boundary of gene expression. The QTLs identified in this study provide a map to guide future reverse genetic and functional studies examining the genetic basis and evolutionary significance of this trait.

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In Vitro Culture of Floral Buds of Aquilegia

Cited by 36 publications

References 12 publications

Morphogenic Potentialities of Flower Buds of Kalanchoe pinnata Pers. grown in vitro

Morphogenic Potentialities of Flower Buds of Kalanchoe pinnata Pers. grown in vitro

Floral Organogenesis in Three Members of the Tribe Delphinieae (Ranunculaceae)

Loss of staminodes in Aquilegia jonesii reveals a fading stamen-staminode boundary

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