In Vitro Culture of Chestnut (Castanea Sativa Mill.) Using Temporary Immersion Bioreactors

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

“…1g). High levels of hyperhydricity were also observed by Troch et al (2010), which considered this disorder as a major obstacle to culture European chestnut shoots in liquid medium. Hyperhydric shoots have not been observed during the culture of other tree species in TIS, such as Crescentia cujete (Murch et al 2004) and willow (Vidal et al 2015).…”

“…The use of 0.22 lM BA produced shoots with some non-hyperhydric leaves, but completely normal shoots were not obtained in any treatment. Likewise, the manipulation of the frequency and duration of immersions did not prevent hyperhydricity in European chestnut shoots cultured in liquid medium by Troch et al (2010). Different methods for maintaining the explants in an upright position were tested with clones 111, 7521, 1483, and 90025.…”

“…(pistachio) (Murch et al 2004;McAlister et al 2005;Chakrabarty et al 2003;Zhu et al 2005;Debnath 2009;Quiala et al 2012;Moreno et al 2012;Akdemir et al 2014). Troch et al (2010) performed several experiments to micropropagate axillary shoots of European chestnut in liquid medium using bioreactors of the twin flask system (Escalona et al 1999). However, no multiplication could be achieved by these authors, as new shoots were not produced in the bioreactors (Troch et al 2010).…”

“…Troch et al (2010) performed several experiments to micropropagate axillary shoots of European chestnut in liquid medium using bioreactors of the twin flask system (Escalona et al 1999). However, no multiplication could be achieved by these authors, as new shoots were not produced in the bioreactors (Troch et al 2010). In a former study of our research group, we explored the propagation of Salix viminalis (willow) and chestnut in liquid medium, either with temporary or continuous immersion systems.…”

Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>® </sup> Bioreactors and <i>ex Vitro</i> Rooting

“…This systems have been used to culture somatic embryos of different woody species [18]; some of them from temperate zones such us Kalopanax septemlobus [19], Quercus robur [20], Q. suber [21]; others correspond to woody tropical as Hevea brasiliensis [22] [23], Coffea arabica [24], Coffea canephora [25], Theobroma cacao [26], Elaeis guineensis [27], and Bactris gasipaes [28]. Although there are some reports on the micropropagation of woody plants using the culture of nodal explants in temporary immersion, including Eucalyptus [29] [30] [31], Crescentia cujete [32], Malus [33], Castanea sativa [18] [34], Gmelina arborea [35], Psidium guajava [36] and Pistacia [37]; only two of them correspond to Tectona grandis [14] [38].…”