In-Vitro Culture Morphology of Spermatogonial Stem Cells (SSCs) in Mice

Ram, Kaavya; Kannan, T.; Basha, Sabiha Hayath; GeethaRamesh,; William, B. Justin

doi:10.5455/ijlr.20170130065836

Cited by 1 publication

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“…Although no consistent bull SSCs markers have been determined, UCHL1 and PLZF have been specifically detected in bull SSCs, as reported in several studies [ 13 , 40 , 41 , 42 ]. UCHL1 is related to the events of self-renewal and colocalization of SSCs and is specifically expressed in vivo in SSCs located in the basement membrane of seminiferous tubules, but not in differentiated GCs [ 43 , 44 ]. PLZF is a conserved marker of undifferentiated GCs in animal model including cattle, sheep, and goats [ 45 , 46 ].…”

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confidence: 99%

Bovine Peripheral Blood-Derived Mesenchymal Stem Cells (PB-MSCs) and Spermatogonial Stem Cells (SSCs) Display Contrasting Expression Patterns of Pluripotency and Germ Cell Markers under the Effect of Sertoli Cell Conditioned Medium

Segunda,

Díaz,

Torres

et al. 2024

Animals

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In vitro gamete derivation has been proposed as an interesting strategy for treatment of infertility, improvement of genetic traits, and conservation of endangered animals. Spermatogonial stem cells (SSCs) are primary candidates for in vitro gamete derivation; however, recently, mesenchymal stem cells (MSCs) have also been proposed as candidates for germ cell (GCs) differentiation mainly due to their transdifferentiating capacity. The objective of the present study was to compare the potential for GC differentiation of bovine peripheral blood-derived MSCs (PB-MSCs) and SSCs under the effect of conditioned medium (CM) derived from Sertoli cells (SCs/CM). Samples were collected every 7 days for 21 days and analyzed for pluripotent, GC, and MSC marker expression. The absence of OCT4 and the increased (p < 0.05) expression of NANOG seems to play a role in SSC differentiation, whereas the absence of NANOG and the increased expression (p < 0.05) of OCT4 may be required for PB-MSC differentiation into GCs. SSCs cultured with SCs/CM increased (p < 0.05) the expression of PIWIL2 and DAZL, while PB-MSCs cultured under the same condition only increased (p < 0.05) the expression of DAZL. Overall, the patterns of markers expression suggest that PB-MSCs and SSCs activate different signaling pathways after exposure to SCs/CM and during differentiation into GCs.

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