In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates

Zweygarth, E.; Cabezas-Cruz, Alejandro; Josemans, Antoinette I.; Oosthuizen, Marinda C.; Matjila, Paul Tshepo; Lis, Katarzyna; Broniszewska, Marzena; Schöl, Heidrun; Ferrolho, Joana; Grubhoffer, Libor; Passos, Lygia M. F.

doi:10.1016/j.ttbdis.2014.01.011

Cited by 26 publications

(37 citation statements)

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“…Doyle et al (2005) characterized the TRP36 gene of American (US), Brazilian (Br) and Cameroonian E. canis strains and reported a Tandem Repeat (TR) sequence of nine amino acids (TEDSVSAPA) (US genotype; Table 1). This TR amino acid sequence was completely preserved in numerous E. canis strains around the world (Zhang et al, 2008;Hsieh et al, 2010;Kamani et al, 2013), including the new isolates from South Africa reported by Zweygarth et al (2014). Only one divergent E. canis TR sequence has been identified in an Israeli strain (Ranana strain) "TEDPVSATA".…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 92%

“…In addition, the number of TR varied from 4 to 18 copies. According to Zweygarth et al (2014), strains from Taiwan and South Africa presented 142 amino acids in the N-Terminus region (pre-TR region), unlike the other strains, which presented 143 amino acids in the N-Termini (Table 2). Subsequently, phylogenetic analysis of South-African and Taiwan strains of E. canis classified them within the same clade.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…Subsequently, phylogenetic analysis of South-African and Taiwan strains of E. canis classified them within the same clade. However, Zweygarth et al (2014) did not consider our paper about E. canis diversity in Brazil . Our results clearly showed a new E. canis genogroup that had a TR sequence of eight amino acids "ASVVPEAE" varying from 6 to15 copies in isolates obtained of naturally infected dogs from various regions in Brazil.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

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Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Aguiar

Melo

2015

Ticks and Tick-borne Diseases

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Section: Contents Lists Available At Sciencedirectmentioning

confidence: 92%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

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Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Aguiar

Melo

2015

Ticks and Tick-borne Diseases

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“…UFMG-EV was isolated from R. microplus hemolymph [2]. Secondly, while E. canis is mainly pathogenic for dog [10], Ehrlichia sp. UFMT-BV was found to be pathogenic for cattle [5].…”

Section: Model Of Emergence Of Ehrlichia Sp Ufmg-ev and Ehrlichia Spmentioning

confidence: 99%

“…The gene trp36 has been widely used to study the genetic diversity of E. canis strains [7][8][9][10]. We performed maximum likelihood and neighbor joining phylogenetic analyses with trp36 nucleotide sequences available in GenBank (Additional file 1) to study the evolution of Ehrlichia sp.…”

mentioning

confidence: 99%

The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection

Cabezas‐Cruz

Valdés

Fuente

2014

Parasites & Vectors

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Background: A new species of Ehrlichia, phylogenetically distant from E. ruminantium, was found in 2010 infecting cattle in Canada. In 2012 and 2013, we reported the in vitro propagation, molecular and ultrastructural characterization of Ehrlichia sp. UFMG-EV (E. mineirensis), a new species of Ehrlichia isolated from the haemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks. A new organism, named Ehrlichia sp. UFMT-BV, closely related to Ehrlichia sp. UFMG-EV, was recently described in Brazil and after experimental infection it was shown to be pathogenic for cattle. This new emerging clade of cattle Ehrlichia pathogens is closely related to E. canis. The major immunogenic Tandem Repeat Protein (TRP36; also known as gp36) is extensively used to characterize the genetic diversity of E. canis. Homologs of TRP36 were found in both Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV. Findings: Herein, we characterized the evolution of this new Ehrlichia clade using TRP36 sequences. Our working hypothesis is that Ehrlichia sp. UFMG-EV and related microorganisms evolved from a highly variable E. canis clade. In support of our hypothesis we found that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV TRP36 evolved from a highly divergent and variable clade within E. canis and this clade evolved under episodic diversifying selection with a high proportion of sites under positive selection. Conclusion: Our results suggest that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV evolved from a variable clade within E. canis.

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Determination of Serum Oxidative Stress, Antioxidant Capacity and Protein Profiles in Dogs Naturally Infected with Ehrlichia canis

et al. 2021

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In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates

Cited by 26 publications

References 43 publications

Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection

Determination of Serum Oxidative Stress, Antioxidant Capacity and Protein Profiles in Dogs Naturally Infected with Ehrlichia canis

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