In vitro culture and cloning of Toxoplasma gondii in a newly established cell line derived from TG180

Couatarmanach, Antoine; André, Pascal; Minous, D. Le; Martin, L. A.; Robert, Raymond; Deunff, J.

doi:10.1016/0020-7519(91)90133-r

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…A host cell-parasite ratio of 3 : 1 was used, as this ratio has been shown to yield a large number of intracellular parasites (Couatarmanach et al, 1991). The cell suspensions were collected from the flasks daily, by centrifugation at 1,500 rpm for 5 min from day 1 to day 6 post-infection (PI).…”

Section: Infection Of Sarcoma 180 Cells With the Ki-1mentioning

confidence: 99%

Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea

et al. 2003

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Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.

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Section: Infection Of Sarcoma 180 Cells With the Ki-1mentioning

confidence: 99%

Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea

et al. 2003

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“…The TG180 murine sarcoma cells are commonly utilized to produce T. gondii by co-inoculation in the peritoneal cavity of mice. Couatarmanach et al (1991) demonstrated that the culture of TG180 in vitro could produce large amounts of T. gondii, up to 259 parasites/cell. Therefore, this method of culture can be very useful to evaluate the effect of new drugs against T. gondii.…”

Section: Discussionmentioning

confidence: 99%

“…TG180 murine sarcoma cell cultures -The TG180 cell line was derived from the ATCC (CCRFS-180 II) sarcoma 180 in 1958 (Sartorelli (Couatarmanach et al 1991) was used in our experiments. For the in vitro experiments the cells were grown in MEM supplemented with 10% fetal calf serum, 40 µg/ml gentamycin and 25 µg/ml amphotericin B.…”

Section: Methodsmentioning

confidence: 99%

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Ultrastructural study of the TG180 murine sarcoma cell invasion by Toxoplasma gondii: comparison between in vivo and in vitro cell cultures

Barbosa

Silva

Ferro

et al. 2000

Mem. Inst. Oswaldo Cruz

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“…Chlamydia trachomatis (Ct) isolated from a clinical specimen was maintained in the murine L cell line. Toxoplasma gondii (Tg) was cultured in a murine cell line as described previously (8). PCR All PCR were performed in standard conditions: 500 ng of genomic DNA per 100 tdl reaction, 1 yM of each primer, 200 ,tM of each dNTP, 50 mM KCI, 2 mM MgCl2, 0.01% gelatine, in a Tris 10 mM pH 8.4 buffer, with TAQ polymerase (2U/100 Electrophoresis of PCR products obtained from the DNA of HSB2 cells (human lineage) by primers possessing at their 3' end rare and frequent human octamers.…”

Section: Dnasmentioning

confidence: 99%

K-tuple frequency in the human genome and polymerase chain reaction

Griffais

André

Thibon³

1991

Nucl Acids Res

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The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers to amplify DNA target sequences, either of human genes or of human infectious agents. The software also gave nested primer sequences which were used to synthesize non radioactive probes by PCR. We applied these two methods, primer selection and non radioactive probes, to easily and quickly set up very efficient PCR sets to work in the human genome context.

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In vitro culture and cloning of Toxoplasma gondii in a newly established cell line derived from TG180

Cited by 8 publications

References 11 publications

Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea

Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea

Ultrastructural study of the TG180 murine sarcoma cell invasion by Toxoplasma gondii: comparison between in vivo and in vitro cell cultures

K-tuple frequency in the human genome and polymerase chain reaction

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