“…1999). Either a scalpel blade or a mechanical tissue chopper adjusted to 500 μm was used to slice the ovarian cortex (Saha et al. 1999).…”
Section: Cattlementioning
confidence: 99%
“…Various additives like FSH (Hulshof et al. 1995), ITS (Saha et al. 1999), growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF) (Wandji et al.…”
Section: Cattlementioning
confidence: 99%
“…In cattle, the mechanical method has been the common method of isolation of PFs (Ralph et al 1996;Saha et al 1999;Itoh and Hoshi 2000). The most common procedure has been the microdissection of the cortical slices of ovaries (Ralph et al 1996;Katska and Rynska 1998), which is a relatively more time-consuming procedure (Telfer et al 1999).…”
Section: Cattlementioning
confidence: 99%
“…The most common procedure has been the microdissection of the cortical slices of ovaries (Ralph et al 1996;Katska and Rynska 1998), which is a relatively more time-consuming procedure (Telfer et al 1999). Either a scalpel blade or a mechanical tissue chopper adjusted to 500 lm was used to slice the ovarian cortex (Saha et al 1999). Enzymes such as collagenase and DNase have been used in the past to isolate PFs from cattle ovaries (Figueiredo et al 1993;Wandji et al 1996).…”
Section: Cattlementioning
confidence: 99%
“…Large PFs were shown to have more growth than the small PFs in culture (Katska and Rynska 1998). Various additives like FSH (Hulshof et al 1995), ITS (Saha et al 1999), growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF) (Wandji et al 1996), and vasoactive intestinal polypeptide (VIP) (Van den Hurk et al 1997) were supplemented to develop suitable culture media for cattle PFs in bovines. The development of bovine PFs up to antral stage was achieved by culturing PFs (166 lm) up to 28 days (Gutierrez et al 2000).…”
The development of efficient ovarian preantral follicle (PF) isolation and culture systems provide a large number of oocytes for the manipulation and embryo production. It also helps for understanding the mechanisms of follicle and oocyte development. Isolation and culture protocols for PFs were developed for many domestic species like cattle, buffalo, sheep, goat, pig, horse, camel, dog and cats; however, embryo production from oocytes derived from in vitro grown PFs was reported only in pigs, buffalo, sheep and goat. The rate of oocyte maturation from PFs grown in vitro is low and requires considerable research. This paper presents an overview of isolation and culture systems of PFs that have been developed for domestic species (cattle, buffalo, sheep, goat, pigs, horse, camel, dog and cat) along with the current status of progress achieved in the direction of producing embryos using PFs as the source of oocyte in these species.
“…1999). Either a scalpel blade or a mechanical tissue chopper adjusted to 500 μm was used to slice the ovarian cortex (Saha et al. 1999).…”
Section: Cattlementioning
confidence: 99%
“…Various additives like FSH (Hulshof et al. 1995), ITS (Saha et al. 1999), growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF) (Wandji et al.…”
Section: Cattlementioning
confidence: 99%
“…In cattle, the mechanical method has been the common method of isolation of PFs (Ralph et al 1996;Saha et al 1999;Itoh and Hoshi 2000). The most common procedure has been the microdissection of the cortical slices of ovaries (Ralph et al 1996;Katska and Rynska 1998), which is a relatively more time-consuming procedure (Telfer et al 1999).…”
Section: Cattlementioning
confidence: 99%
“…The most common procedure has been the microdissection of the cortical slices of ovaries (Ralph et al 1996;Katska and Rynska 1998), which is a relatively more time-consuming procedure (Telfer et al 1999). Either a scalpel blade or a mechanical tissue chopper adjusted to 500 lm was used to slice the ovarian cortex (Saha et al 1999). Enzymes such as collagenase and DNase have been used in the past to isolate PFs from cattle ovaries (Figueiredo et al 1993;Wandji et al 1996).…”
Section: Cattlementioning
confidence: 99%
“…Large PFs were shown to have more growth than the small PFs in culture (Katska and Rynska 1998). Various additives like FSH (Hulshof et al 1995), ITS (Saha et al 1999), growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF) (Wandji et al 1996), and vasoactive intestinal polypeptide (VIP) (Van den Hurk et al 1997) were supplemented to develop suitable culture media for cattle PFs in bovines. The development of bovine PFs up to antral stage was achieved by culturing PFs (166 lm) up to 28 days (Gutierrez et al 2000).…”
The development of efficient ovarian preantral follicle (PF) isolation and culture systems provide a large number of oocytes for the manipulation and embryo production. It also helps for understanding the mechanisms of follicle and oocyte development. Isolation and culture protocols for PFs were developed for many domestic species like cattle, buffalo, sheep, goat, pig, horse, camel, dog and cats; however, embryo production from oocytes derived from in vitro grown PFs was reported only in pigs, buffalo, sheep and goat. The rate of oocyte maturation from PFs grown in vitro is low and requires considerable research. This paper presents an overview of isolation and culture systems of PFs that have been developed for domestic species (cattle, buffalo, sheep, goat, pigs, horse, camel, dog and cat) along with the current status of progress achieved in the direction of producing embryos using PFs as the source of oocyte in these species.
Congestive heart failure due to mitral regurgitation in an Indian hill mynah bird.ONLY a small number of oocytes within an ovary are present in antral follicles. Many more oocytes, however, are present in preantral follicles, most of which are left unutilised over the reproductive life of an animal. Harvesting oocytes only from antral follicles therefore limits the number of offspring per animal and thus the optimal exploitation of female genetic material (van den Hurk and others 1997). Hence, recent efforts have been made to develop the techniques of isolation of preantral follicles in animals. The ability to harvest oocytes from preantral follicles will facilitate basic research into the control of early development, provide a source of high quality oocytes for nuclear transfer and transgenic technologies and, in the case of buffalo, develop the potential of commercial embryo transfer.The reproductive capacity of buffalo is relatively poor in comparison with cattle. Buffalo have fewer primordial follicles (Danell 1987) and a higher rate of follicular atresia (Bharadwaz and Roy 1999). The buffalo ovary contains thousands of oocytes enclosed primarily in preantral follicles but only a few mature and reach the ovulatory stage. To tap the unutilised preantral follicles for oocyte production, techniques need to be developed for the isolation of preantral folMechanical Enzymatic Hyaluronidase Trypsin Fresh ovaries Stored ovaries Fresh ovaries Stored ovaries Fresh ovaries Number of ovaries 70 55 10 6 4 Mean (se) number of PF recovered per ovary 3*90 (0-40)a 2-58 (0-46)a 20-25 (4-42)b 16-53 (5-06)b 21-50 (O)b Values in a row with different superscripts differ significantly (P<0-001) FIG 1: Preantral follicles isolated from buffalo ovaries (x 200) licles from buffalo ovaries as they have been for domestic animals such as cattle (Hulshof and others 1995, Wandji and others 1996), sheep (Cecconi and others 1999), goats (Chelikani and others 1998) and pigs (Morbeck and others 1993).However, such studies on buffalo are lacking. In this study, preantral follicles were isolated from abattoir derived buffalo ovaries using both mechanical and enzymatic methods. The efficiency of both methods was compared. A total of 145 buffalo ovaries were collected from the local abattoir on 27 different days between May and December 1998. The ovaries were collected in normal saline containing gentamicin (50 .g/ml) and were brought to the laboratory within one hour of slaughter. Extraovarian tissue was removed and the ovaries were washed thoroughly with Dulbecco's phosphate buffered saline. Some ovaries (84) were used immediately for isolation of preantral follicles and the rest were frozen overnight at 0°C for isolation on the following day. Preantral follicles were isolated by two methods, a mechanical method and an enzymatic method.In the mechanical method, the ovary was first halved with a surgical blade and the medulla was scraped off. The cortex was then sliced into pieces of approximately 1 cm3. These pieces of ovary were washed with a ...
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