In vitro cultivation of Schistosoma mansoni

Clegg, J. A.

doi:10.1016/0014-4894(65)90037-8

Cited by 214 publications

(140 citation statements)

References 12 publications

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“…It should be realized, however, that cercariae in isotonic medium at 37 "C could be kept in good condition only for a few hours (=lo h), and at 48 h they become motionless. In contrast, schistosomula were kept for 45 days under identical culture condition and they developed into nearly mature adult worms (Clegg, 1965). It seems, therefore, that tail removal associated with hsp70 gene transcription is necessary for late stages of transformation and for parasite maintenance thereafter.…”

Section: Discussionmentioning

confidence: 99%

Regulation of HSP70 gene expression during the life cycle of the parasitic helminth Schistosoma mansoni

Neumann

Ziv

Lantner

et al. 1993

European Journal of Biochemistry

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Analyses of RNA from different developmental stages of Schistosoma mansoni showed stagespecific expression of heat-shock protein 70 (hsp70), which is regulated by a developmental program and by stress. The developmental program, common to hsp70 and other genes (e. g. paramyosin), refers to constitutive expression in miracidia sporocyst and adult worm but not in cercariae, and to the termination of hsp70 gene transcription during sporocystkercaria transformation. Stress induction, specific to hsp70, refers to transient accumulation of high levels of hsp70 mRNA during cercariae/schistosomula transformation and in adult worms after heat shock (42 "C). Cercariae/ schistosomula transformation can be visualized as a physiological stress involving shifts in temperature (23-37 "C) and in salt concentration (from water to isotonic medium), as well as removal of tails from cercariae to yield isolated bodies that transform into schistosomula. It was found that temperature is an important factor, but not sufficient for strong induction of the hsp70 genes of schistosomula. Tail removal is an obligatory step for full induction of the hsp70 genes of schistosomula, in response to a temperature shift from 23 -37 "C. The hsp70 genes in cercariae and isolated tails do not respond to stimuli (salt and temperature increases) that strongly activate the genes in isolated bodies (i. e., schistosomula). We speculate that the hsp70 genes in intact cercariae are not inducible because the tails can produce inhibitory signals that diffuse to the bodies and suppress their hsp70 genes. This hypothesis is useful to explain the termination of hsp70 gene transcription during sporocystkercaria transformation by the inhibitory effect of the growing tail.

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Section: Discussionmentioning

confidence: 99%

Regulation of HSP70 gene expression during the life cycle of the parasitic helminth Schistosoma mansoni

Neumann

Ziv

Lantner

et al. 1993

European Journal of Biochemistry

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“…The underlying rationale for selecting these two time points, i.e., urine collection 49 and 56 days after infection, was our interest in the metabolic profiling of an adult S. mansoni infection. Previous work had established that the development of schistosomes to reach sexual maturity and, hence, commence with egg production, takes Ն34-35 days, whereas in some worms it may take an additional 20 days (23). Finally, urine samples were also obtained from uninfected control mice at the same time points and adhering to the same procedures.…”

Section: Methodsmentioning

confidence: 99%

Metabonomic investigations in mice infected with Schistosoma mansoni : An approach for biomarker identification

Wang

Holmes

Nicholson

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

278

289

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asis. Using a Schistosoma mansoni-mouse model, we present a characterization of a parasitic infection by metabolic profiling, employing 1 H NMR spectroscopy and multivariate pattern recognition techniques. We infected 10 mice with 80 S. mansoni cercariae each and collected urine samples 49 and 56 days postinfection. Urine samples were also obtained from 10 uninfected control mice at the same time. The metabolic signature of an S. mansoni infection consists of reduced levels of the tricarboxylic acid cycle intermediates, including citrate, succinate, and 2-oxoglutarate, and increased levels of pyruvate, suggesting stimulated glycolysis. A disturbance of amino acid metabolism was also associated with an S. mansoni infection, as indicated by depletion of taurine, 2-oxoisocaproate, and 2-oxoisovalerate and elevation of tryptophan in the urine. A range of microbial-related metabolites, i.e., trimethylamine, phenylacetylglycine, acetate, p-cresol glucuronide, butyrate, propionate, and hippurate, were also coupled with an S. mansoni infection, indicating disturbances in the gut microbiota. Our work highlights the potential of metabolic profiling to enhance our understanding of biological responses to parasitic infections. It also holds promise as a basis for novel diagnostic tests with high sensitivity and specificity and for improved disease surveillance and control.

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“…In parallel to the development of schistosomula culture systems, culture methods for adult worms have been established (Clegg, 1965 ;Ramirez et al 2007). Today, in vitro chemotherapeutic studies mainly rely on S. mansoni adults (Table 1, Fig.…”

Section: Adult S Mansoni In Vitro Assaymentioning

confidence: 99%

In vitroandin vivotrematode models for chemotherapeutic studies

2009

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Schistosomiasis and food-borne trematodiases are chronic parasitic diseases affecting millions of people mostly in the developing world. Additional drugs should be developed as only few drugs are available for treatment and drug resistance might emerge. In vitro and in vivo whole parasite screens represent essential components of the trematodicidal drug discovery cascade. This review describes the current state-of-the-art of in vitro and in vivo screening systems of the blood fluke Schistosoma mansoni, the liver fluke Fasciola hepatica and the intestinal fluke Echinostoma caproni. Examples of in vitro and in vivo evaluation of compounds for activity are presented. To boost the discovery pipeline for these diseases there is a need to develop validated, robust high-throughput in vitro systems with simple readouts.

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In vitro cultivation of Schistosoma mansoni

Cited by 214 publications

References 12 publications

Regulation of HSP70 gene expression during the life cycle of the parasitic helminth Schistosoma mansoni

Regulation of HSP70 gene expression during the life cycle of the parasitic helminth Schistosoma mansoni

Metabonomic investigations in mice infected with Schistosoma mansoni : An approach for biomarker identification

In vitroandin vivotrematode models for chemotherapeutic studies

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