In vitro cultivation of Eimeria acervulina (Coccidia)

Strout, Richard G.; Solis, Juan; Smith, Samuel C.; Dunlop, W.

doi:10.1016/0014-4894(65)90064-0

Cited by 33 publications

(14 citation statements)

References 14 publications

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“…The refractile bodies (eosinophilic globules) are characteristically present in the sporozoites and are also frequently found in first-generation schizonts of Eimeria species. They were seen in trophowites and/or schizonts grown in cell cultures in E. tenella(l3), E. acervulina ( 15) and E. meleagrimitis (2). The refractile bodies are protein in nature (5,12).…”

Section: Discussionmentioning

confidence: 99%

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Development of First‐Generation Schizonts of Eimeria bovis in Cultured Bovine Cells

Fayer

Hammond

1967

The Journal of Protozoology

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SYNOPSIS. Monolayer primary and secondary cultures of embryonic bovine kidney, spleen, intestinal and testicle cells, and secondary cultures of embryonic bovine thymus, maintained in lactalbumin hydrolysate, Earle's balanced salt solution and ovine serum were observed for a maximum of 21 days after inoculation of E. bovis sporozoites. The sporozoites entered the cells in all of these cultures but underwent development only in primary cultures of kidney and intestinal cells and in secondary cultures of kidney, spleen, thymus, intestinal, and testicle cells. In acellular media, the sporozoites retained motility no longer than 21 hr. In the cell cultures, free motile sporozoites were seen for as long as 18 days after inoculation. Sporozoites entered cells anterior end first; the process of penetration required a few seconds to about a minute. Sporozoites were also observed leaving host cells. Intracellular sporozoites were first seen 3 min after inoculation; they were observed at various intervals up to 18 days after inoculation. In transformation of sporozoites into trophozoites a marked change in size and appearance of the nucleus took place before the change in shape of the body occurred. Trophozoites were first found 7 days after inoculation, multinucleate schizonts after 8 days, and schizonts with merozoites after 14 days. Schizonts containing merozoites were seen only in kidney, spleen, and thymus cells. The mature schizonts were smaller and represented a much lower proportion of the total number than in comparable stages of infections in calves. Schizonts with many nuclei occurred in intestinal cells; the most advanced stage seen in testicle cells was the binucleate schizont. Nuclear and cytoplasmic changes were observed in the infected cells.

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Section: Discussionmentioning

confidence: 99%

“…Strout et a1. (15) obtained cell infection with E. acervulinu in cultures of chick embryo kidney, chick embryo fibroblasts, mouse fibroblasts, human amnion and HeLa cells. Trophozoites were observed in all cell types and stages of segmentation of trophozoites were observed in a few instances.…”

Section: Development Of First-generation Schizontsmentioning

confidence: 99%

Development of First‐Generation Schizonts of Eimeria bovis in Cultured Bovine Cells

Fayer

Hammond

1967

The Journal of Protozoology

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“…In most currently tested cell lines, E. tenella develops only asexually, halting at the first generation of schizont in cultures of mammalian fibroblasts, mammalian epithelial cells, and avian fibroblasts [ 8 , 26 ]. In vitro invasion of chick embryo kidney, chick embryo fibroblast, mouse fibroblast, human amnion, and HeLa cell cultures by E. acervulina sporozoites do not lead to further parasite development [ 27 ]. However, studies using, non-poultry species of Eimeria (e.g.…”

Section: Discussionmentioning

confidence: 99%

Establishment of an in vitro chicken epithelial cell line model to investigate Eimeria tenella gamete development

et al. 2018

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BackgroundEimeria tenella infection leads to acute intestinal disorders responsible for important economic losses in poultry farming worldwide. The life-cycle of E. tenella is monoxenous with the chicken as the exclusive host; infection occurs in caecal epithelial cells. However, in vitro, the complete life-cycle of the parasite has only been propagated successfully in primary chicken kidney cells, which comprise undefined mixed cell populations; no cell line model has been able to consistently support the development of the sexual stages of the parasite. We therefore sought to develop a new model to study E. tenella gametogony in vitro using a recently characterised chicken cell line (CLEC-213) exhibiting an epithelial cell phenotype.MethodsCLEC-213 were infected with sporozoites from a precocious strain or with second generation merozoites (merozoites II) from wild type strains. Sexual stages of the parasite were determined both at the gene and protein levels.ResultsTo our knowledge, we show for the first time in CLEC-213, that sporozoites from a precocious strain of E. tenella were able to develop to gametes, as verified by measuring gene expression and by using antibodies to a microgamete-specific protein (EtFOA1: flagellar outer arm protein 1) and a macrogamete-specific protein (EtGAM-56), but oocysts were not observed. However, both gametes and oocysts were observed when cells were infected with merozoites II from wild type strains, demonstrating that completion of the final steps of the parasite cycle is possible in CLEC-213 cells.ConclusionThe epithelial cell line CLEC-213 constitutes a useful avian tool for studying Eimeria epithelial cell interactions and the effect of drugs on E. tenella invasion, merogony and gametogony.

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“…O Centro Técnico de Reprodução de Eimerias, do Laboratório BIOVET, Brasil, executou a purifi cação e ajuste do isolado (STROUT et al, 1965 Amostras de fezes e cama contidas em cada grupo foram coletadas diariamente, sendo obtidas em seis locais dos boxes experimentais (quatro amostras laterais e duas centrais), para determinação do período pré patente e curva de eliminação de oocistos. Realizou-se a técnica de SHEATER (1923) modifi cada, com as amostras de fezes e os oocistos recuperados foram quantifi cados em câmara de McMaster.…”

Section: Methodsunclassified

Desinfecção com amônia quaternária associada à fermentação não potencializa o controle de coccidiose em cama de frango

2013

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In vitro cultivation of Eimeria acervulina (Coccidia)

Cited by 33 publications

References 14 publications

Development of First‐Generation Schizonts of Eimeria bovis in Cultured Bovine Cells

Development of First‐Generation Schizonts of Eimeria bovis in Cultured Bovine Cells

Establishment of an in vitro chicken epithelial cell line model to investigate Eimeria tenella gamete development

Desinfecção com amônia quaternária associada à fermentação não potencializa o controle de coccidiose em cama de frango

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